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| - | [[Image:2ds3.gif|left|200px]] | + | #REDIRECT [[3d3i]] This PDB entry is obsolete and replaced by 3d3i |
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| - | {{Structure
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| - | |PDB= 2ds3 |SIZE=350|CAPTION= <scene name='initialview01'>2ds3</scene>, resolution 1.78Å
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| - | |SITE=
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| - | |LIGAND= <scene name='pdbligand=CA:CALCIUM+ION'>CA</scene>, <scene name='pdbligand=GOL:GLYCEROL'>GOL</scene>, <scene name='pdbligand=MSE:SELENOMETHIONINE'>MSE</scene>
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| - | |ACTIVITY=
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| - | |GENE= ygjK ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=562 Escherichia coli])
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| - | |DOMAIN=
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| - | |RELATEDENTRY=
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| - | |RESOURCES=<span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=2ds3 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=2ds3 OCA], [http://www.ebi.ac.uk/pdbsum/2ds3 PDBsum], [http://www.rcsb.org/pdb/explore.do?structureId=2ds3 RCSB]</span>
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| - | }}
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| - | '''Crystal structure of E. coli YgjK, a member of glycosyl hydrolase family 63'''
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| - | ==Overview==
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| - | Processing alpha-glucosidase I, which is classified into glycosyl hydrolase (GH) family 63, hydrolyzes an oligosaccharide precursor of eukaryotic N-linked glycoproteins. Recently, many bacteria have been reported to possess genes for proteins that are homologous to the GH family 63 glucosidases. In this paper, Escherichia coli K12 YgjK protein, a member of GH family 63, was overexpressed, purified and crystallized using the vapour-diffusion method. Diffraction data were collected to 1.8 A resolution and the crystal was found to belong to the monoclinic space group P2(1), with unit-cell parameters a = 88.5, b = 137.1, c = 60.9 A, beta = 98.1 degrees. The V(M) value was determined to be 2.1 A(3) Da(-1), which corresponds to the presence of two protein molecules in the asymmetric unit.
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| - | ==About this Structure==
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| - | 2DS3 is a [[Single protein]] structure of sequence from [http://en.wikipedia.org/wiki/Escherichia_coli Escherichia coli]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2DS3 OCA].
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| - | ==Reference==
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| - | Crystallization and preliminary X-ray analysis of Escherichia coli K12 YgjK protein, a member of glycosyl hydrolase family 63., Tonozuka T, Uechi A, Mizuno M, Ichikawa K, Nishikawa A, Sakano Y, Acta Crystallogr D Biol Crystallogr. 2004 Jul;60(Pt 7):1284-5. Epub 2004, Jun 22. PMID:[http://www.ncbi.nlm.nih.gov/pubmed/15213393 15213393]
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| - | [[Category: Escherichia coli]]
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| - | [[Category: Single protein]]
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| - | [[Category: Kurakata, Y.]]
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| - | [[Category: Tonozuka, T.]]
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| - | [[Category: escherichia coli]]
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| - | [[Category: glycosyl hydrolase family 63]]
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| - | [[Category: processing alpha-glucosidase]]
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| - | ''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Mon Mar 31 02:38:42 2008''
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