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5mnd

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SFX structure of Cydia pomonella granulovirus using a double flow-focusing nozzle

Structural highlights

5mnd is a 1 chain structure with sequence from Cydia pomonella granulosis virus (isolate Mexican). Full crystallographic information is available from OCA. For a guided tour on the structure components use FirstGlance.
Method:	X-ray diffraction, Resolution 2.56Å
Resources:	FirstGlance, OCA, PDBe, RCSB, PDBsum, ProSAT

Function

GRAN_GVCPM Component of the virus occlusion bodies, which are large proteinaceous structures, that protect the virus from the outside environment for extended periods until they are ingested by insect larvae.

Publication Abstract from PubMed

Serial femtosecond crystallography requires reliable and efficient delivery of fresh crystals across the beam of an X-ray free-electron laser over the course of an experiment. We introduce a double-flow focusing nozzle to meet this challenge, with significantly reduced sample consumption, while improving jet stability over previous generations of nozzles. We demonstrate its use to determine the first room-temperature structure of RNA polymerase II at high resolution, revealing new structural details. Moreover, the double flow-focusing nozzles were successfully tested with three other protein samples and the first room temperature structure of an extradiol ring-cleaving dioxygenase was solved by utilizing the improved operation and characteristics of these devices.

Double-flow focused liquid injector for efficient serial femtosecond crystallography.,Oberthuer D, Knoska J, Wiedorn MO, Beyerlein KR, Bushnell DA, Kovaleva EG, Heymann M, Gumprecht L, Kirian RA, Barty A, Mariani V, Tolstikova A, Adriano L, Awel S, Barthelmess M, Dorner K, Xavier PL, Yefanov O, James DR, Nelson G, Wang D, Calvey G, Chen Y, Schmidt A, Szczepek M, Frielingsdorf S, Lenz O, Snell E, Robinson PJ, Sarler B, Belsak G, Macek M, Wilde F, Aquila A, Boutet S, Liang M, Hunter MS, Scheerer P, Lipscomb JD, Weierstall U, Kornberg RD, Spence JC, Pollack L, Chapman HN, Bajt S Sci Rep. 2017 Mar 16;7:44628. doi: 10.1038/srep44628. PMID:28300169^[1]

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.

References

↑ Oberthuer D, Knoska J, Wiedorn MO, Beyerlein KR, Bushnell DA, Kovaleva EG, Heymann M, Gumprecht L, Kirian RA, Barty A, Mariani V, Tolstikova A, Adriano L, Awel S, Barthelmess M, Dorner K, Xavier PL, Yefanov O, James DR, Nelson G, Wang D, Calvey G, Chen Y, Schmidt A, Szczepek M, Frielingsdorf S, Lenz O, Snell E, Robinson PJ, Sarler B, Belsak G, Macek M, Wilde F, Aquila A, Boutet S, Liang M, Hunter MS, Scheerer P, Lipscomb JD, Weierstall U, Kornberg RD, Spence JC, Pollack L, Chapman HN, Bajt S. Double-flow focused liquid injector for efficient serial femtosecond crystallography. Sci Rep. 2017 Mar 16;7:44628. doi: 10.1038/srep44628. PMID:28300169 doi:http://dx.doi.org/10.1038/srep44628

1 Structural highlights
2 Function
3 Publication Abstract from PubMed
4 References

Proteopedia Page Contributors and Editors (what is this?)

OCA

Retrieved from "http://52.214.119.220/wiki/index.php/5mnd"

Categories: Large Structures | Chapman H | Doerner K | Oberthuer D | Xavier PL

@@ Line 1: / Line 1: @@
 ==SFX structure of Cydia pomonella granulovirus using a double flow-focusing nozzle==
-<StructureSection load='5mnd' size='340' side='right' caption='[[5mnd]], [[Resolution|resolution]] 2.56&Aring;' scene=''>
+<StructureSection load='5mnd' size='340' side='right'caption='[[5mnd]], [[Resolution|resolution]] 2.56&Aring;' scene=''>
 == Structural highlights ==
-<table><tr><td colspan='2'>[[5mnd]] is a 1 chain structure with sequence from [http://en.wikipedia.org/wiki/Cpgv Cpgv]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=5MND OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=5MND FirstGlance]. <br>
+<table><tr><td colspan='2'>[[5mnd]] is a 1 chain structure with sequence from [https://en.wikipedia.org/wiki/Cydia_pomonella_granulosis_virus_(isolate_Mexican) Cydia pomonella granulosis virus (isolate Mexican)]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=5MND OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=5MND FirstGlance]. <br>
-</td></tr><tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=5mnd FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=5mnd OCA], [http://pdbe.org/5mnd PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=5mnd RCSB], [http://www.ebi.ac.uk/pdbsum/5mnd PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=5mnd ProSAT]</span></td></tr>
+</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution|Resolution]] 2.56&#8491;</td></tr>
+<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=5mnd FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=5mnd OCA], [https://pdbe.org/5mnd PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=5mnd RCSB], [https://www.ebi.ac.uk/pdbsum/5mnd PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=5mnd ProSAT]</span></td></tr>
 </table>
 == Function ==
-[[http://www.uniprot.org/uniprot/GRAN_GVCPM GRAN_GVCPM]] Component of the virus occlusion bodies, which are large proteinaceous structures, that protect the virus from the outside environment for extended periods until they are ingested by insect larvae.
+[https://www.uniprot.org/uniprot/GRAN_GVCPM GRAN_GVCPM] Component of the virus occlusion bodies, which are large proteinaceous structures, that protect the virus from the outside environment for extended periods until they are ingested by insect larvae.
 <div style="background-color:#fffaf0;">
 == Publication Abstract from PubMed ==
@@ Line 21: / Line 22: @@
 __TOC__
 </StructureSection>
-[[Category: Cpgv]]
+[[Category: Large Structures]]
-[[Category: Chapman, H]]
+[[Category: Chapman H]]
-[[Category: Doerner, K]]
+[[Category: Doerner K]]
-[[Category: Oberthuer, D]]
+[[Category: Oberthuer D]]
-[[Category: Xavier, P L]]
+[[Category: Xavier PL]]
-[[Category: Granulovirus]]
-[[Category: In vivo crystal]]
-[[Category: Nanocrystal]]
-[[Category: Sfx]]
-[[Category: Viral protein]]

5mnd

From Proteopedia

Current revision

SFX structure of Cydia pomonella granulovirus using a double flow-focusing nozzle

Structural highlights

Function

Publication Abstract from PubMed

References

Contents

Proteopedia Page Contributors and Editors (what is this?)

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