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| | ==NMR Structure of the DNA-binding Domain of Cell Cycle Protein, Mbp1(2-124) from Saccharomyces cerevisiae== | | ==NMR Structure of the DNA-binding Domain of Cell Cycle Protein, Mbp1(2-124) from Saccharomyces cerevisiae== |
| - | <StructureSection load='1l3g' size='340' side='right' caption='[[1l3g]], [[NMR_Ensembles_of_Models | 19 NMR models]]' scene=''> | + | <StructureSection load='1l3g' size='340' side='right'caption='[[1l3g]]' scene=''> |
| | == Structural highlights == | | == Structural highlights == |
| - | <table><tr><td colspan='2'>[[1l3g]] is a 1 chain structure with sequence from [http://en.wikipedia.org/wiki/Atcc_18824 Atcc 18824]. Full experimental information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1L3G OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=1L3G FirstGlance]. <br> | + | <table><tr><td colspan='2'>[[1l3g]] is a 1 chain structure with sequence from [https://en.wikipedia.org/wiki/Saccharomyces_cerevisiae Saccharomyces cerevisiae]. Full experimental information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1L3G OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=1L3G FirstGlance]. <br> |
| - | </td></tr><tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=1l3g FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1l3g OCA], [http://pdbe.org/1l3g PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=1l3g RCSB], [http://www.ebi.ac.uk/pdbsum/1l3g PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=1l3g ProSAT]</span></td></tr> | + | </td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">Solution NMR</td></tr> |
| | + | <tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=1l3g FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1l3g OCA], [https://pdbe.org/1l3g PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=1l3g RCSB], [https://www.ebi.ac.uk/pdbsum/1l3g PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=1l3g ProSAT]</span></td></tr> |
| | </table> | | </table> |
| | == Function == | | == Function == |
| - | [[http://www.uniprot.org/uniprot/MBP1_YEAST MBP1_YEAST]] Binds to MCB elements (Mlu I cell cycle box) found in the promoter of most DNA synthesis genes. Transcriptional activation by MBF has an important role in the transition from G1 to S phase. It may have a dual role in that it behaves as an activator of transcription at the G1-S boundary and as a repressor during other stages of the cell cycle. | + | [https://www.uniprot.org/uniprot/MBP1_YEAST MBP1_YEAST] Binds to MCB elements (Mlu I cell cycle box) found in the promoter of most DNA synthesis genes. Transcriptional activation by MBF has an important role in the transition from G1 to S phase. It may have a dual role in that it behaves as an activator of transcription at the G1-S boundary and as a repressor during other stages of the cell cycle. |
| | == Evolutionary Conservation == | | == Evolutionary Conservation == |
| | [[Image:Consurf_key_small.gif|200px|right]] | | [[Image:Consurf_key_small.gif|200px|right]] |
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| | __TOC__ | | __TOC__ |
| | </StructureSection> | | </StructureSection> |
| - | [[Category: Atcc 18824]] | + | [[Category: Large Structures]] |
| - | [[Category: Frenkiel, T A]] | + | [[Category: Saccharomyces cerevisiae]] |
| - | [[Category: Kelly, G]] | + | [[Category: Frenkiel TA]] |
| - | [[Category: Lane, A N]] | + | [[Category: Kelly G]] |
| - | [[Category: McIntosh, P B]] | + | [[Category: Lane AN]] |
| - | [[Category: Nair, M]] | + | [[Category: McIntosh PB]] |
| - | [[Category: Smerdon, S J]] | + | [[Category: Nair M]] |
| - | [[Category: Taylor, I A]] | + | [[Category: Smerdon SJ]] |
| - | [[Category: Cell cycle]]
| + | [[Category: Taylor IA]] |
| - | [[Category: Mlu 1 cell cycle box binding protein]]
| + | |
| - | [[Category: Winged helix-turn-helix protein]]
| + | |
| Structural highlights
Function
MBP1_YEAST Binds to MCB elements (Mlu I cell cycle box) found in the promoter of most DNA synthesis genes. Transcriptional activation by MBF has an important role in the transition from G1 to S phase. It may have a dual role in that it behaves as an activator of transcription at the G1-S boundary and as a repressor during other stages of the cell cycle.
Evolutionary Conservation
Check, as determined by ConSurfDB. You may read the explanation of the method and the full data available from ConSurf.
Publication Abstract from PubMed
The three-dimensional solution structure of the DNA-binding domain of Mlu-1 box binding protein (Mbp1) has been determined by multidimensional NMR spectroscopy. Mbp1 is a cell cycle transcription factor from Saccharomyces cerevisiae and consists of an N-terminal DNA-binding domain, a series of ankyrin repeats, and a heterodimerization domain at the C-terminus. A set of conformers comprising 19 refined structures was calculated via a molecular dynamics simulated annealing protocol using distance, dihedral angle, and residual dipolar coupling restraints derived from either double or triple resonance NMR experiments. The solution structure consists of a six-stranded beta-sheet segment folded against two pairs of alpha-helices in the topology of the winged helix-turn-helix family of proteins and is in agreement with the X-ray structures. In addition, the solution structure shows that the C-terminal tail region of this domain folds back and makes specific interactions with the N-terminal beta-strand of the protein. This C-terminal region is essential for full DNA-binding activity but appears in the X-ray structure to be disordered. The fold-back structure extends the region of positive electrostatic potential, and this may enhance the nonspecific contribution to binding by favorable electrostatic interactions with the DNA backbone.
NMR structure of the DNA-binding domain of the cell cycle protein Mbp1 from Saccharomyces cerevisiae.,Nair M, McIntosh PB, Frenkiel TA, Kelly G, Taylor IA, Smerdon SJ, Lane AN Biochemistry. 2003 Feb 11;42(5):1266-73. PMID:12564929[1]
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.
References
- ↑ Nair M, McIntosh PB, Frenkiel TA, Kelly G, Taylor IA, Smerdon SJ, Lane AN. NMR structure of the DNA-binding domain of the cell cycle protein Mbp1 from Saccharomyces cerevisiae. Biochemistry. 2003 Feb 11;42(5):1266-73. PMID:12564929 doi:10.1021/bi0205247
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