5z67

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'''Unreleased structure'''
 
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The entry 5z67 is ON HOLD
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==Structure of the recombination mediator protein RecF in RecFOR pathway==
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<StructureSection load='5z67' size='340' side='right'caption='[[5z67]], [[Resolution|resolution]] 2.20&Aring;' scene=''>
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== Structural highlights ==
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<table><tr><td colspan='2'>[[5z67]] is a 1 chain structure with sequence from [https://en.wikipedia.org/wiki/Caldanaerobacter_subterraneus_subsp._tengcongensis_MB4 Caldanaerobacter subterraneus subsp. tengcongensis MB4]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=5Z67 OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=5Z67 FirstGlance]. <br>
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</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution|Resolution]] 2.2&#8491;</td></tr>
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<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=5z67 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=5z67 OCA], [https://pdbe.org/5z67 PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=5z67 RCSB], [https://www.ebi.ac.uk/pdbsum/5z67 PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=5z67 ProSAT]</span></td></tr>
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</table>
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== Function ==
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[https://www.uniprot.org/uniprot/RECF_CALS4 RECF_CALS4] The RecF protein is involved in DNA metabolism; it is required for DNA replication and normal SOS inducibility. RecF binds preferentially to single-stranded, linear DNA. It also seems to bind ATP.
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<div style="background-color:#fffaf0;">
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== Publication Abstract from PubMed ==
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RecF is a principal member of the RecF pathway. It interacts with RecO and RecR to initiate homologous recombination by loading RecA recombinases on single-stranded DNA and displacing single-stranded DNA-binding proteins. As an ATP-binding cassette ATPase, RecF exhibits ATP-dependent dimerization and structural homology with Rad50 and SMC proteins. However, the mechanism and action pattern of RecF ATP-dependent dimerization remains unclear. Here, We determined three crystal structures of TTERecF, TTERecF-ATP and TTERecF-ATPS from Thermoanaerobacter tengcongensis that reveal a novel ATP-driven RecF dimerization. RecF contains a positively charged tunnel on its dimer interface that is essential to ATP binding. Our structural and biochemical data indicate that the Walker A motif serves as a switch and plays a key role in ATP binding and RecF dimerization. Furthermore, Biolayer interferometry assay results showed that the TTERecF interacted with ATP and formed a dimer, displaying a higher affinity for DNA than that of the TTERecF monomer. Overall, our results provide a solid structural basis for understanding the process of RecF binding with ATP and the functional mechanism of ATP-dependent RecF dimerization.
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Authors: Tang, Q., Liu, Y.-P., Yan, X.-X.
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ATP-dependent conformational change in ABC-ATPase RecF serves as a switch in DNA repair.,Tang Q, Liu YP, Shan HH, Tian LF, Zhang JZ, Yan XX Sci Rep. 2018 Feb 1;8(1):2127. doi: 10.1038/s41598-018-20557-0. PMID:29391496<ref>PMID:29391496</ref>
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Description: Structure of the recombination mediator protein RecF in RecFOR pathway
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From MEDLINE&reg;/PubMed&reg;, a database of the U.S. National Library of Medicine.<br>
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[[Category: Unreleased Structures]]
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</div>
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[[Category: Tang, Q]]
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<div class="pdbe-citations 5z67" style="background-color:#fffaf0;"></div>
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[[Category: Yan, X.-X]]
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== References ==
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[[Category: Liu, Y.-P]]
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<references/>
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__TOC__
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</StructureSection>
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[[Category: Caldanaerobacter subterraneus subsp. tengcongensis MB4]]
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[[Category: Large Structures]]
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[[Category: Liu Y-P]]
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[[Category: Tang Q]]
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[[Category: Yan X-X]]

Current revision

Structure of the recombination mediator protein RecF in RecFOR pathway

PDB ID 5z67

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