2e1n

From Proteopedia

(Difference between revisions)
Jump to: navigation, search
Current revision (13:48, 13 March 2024) (edit) (undo)
 
(10 intermediate revisions not shown.)
Line 1: Line 1:
-
[[Image:2e1n.gif|left|200px]]
 
-
{{Structure
+
==Crystal structure of the Cyanobacterium circadian clock modifier Pex==
-
|PDB= 2e1n |SIZE=350|CAPTION= <scene name='initialview01'>2e1n</scene>, resolution 1.80&Aring;
+
<StructureSection load='2e1n' size='340' side='right'caption='[[2e1n]], [[Resolution|resolution]] 1.80&Aring;' scene=''>
-
|SITE=
+
== Structural highlights ==
-
|LIGAND= <scene name='pdbligand=SO4:SULFATE+ION'>SO4</scene>
+
<table><tr><td colspan='2'>[[2e1n]] is a 2 chain structure with sequence from [https://en.wikipedia.org/wiki/Synechococcus_elongatus_PCC_7942_=_FACHB-805 Synechococcus elongatus PCC 7942 = FACHB-805]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2E1N OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=2E1N FirstGlance]. <br>
-
|ACTIVITY=
+
</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution|Resolution]] 1.8&#8491;</td></tr>
-
|GENE= pex ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=1131 Synechococcus sp.])
+
<tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=SO4:SULFATE+ION'>SO4</scene></td></tr>
-
|DOMAIN=
+
<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=2e1n FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=2e1n OCA], [https://pdbe.org/2e1n PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=2e1n RCSB], [https://www.ebi.ac.uk/pdbsum/2e1n PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=2e1n ProSAT]</span></td></tr>
-
|RELATEDENTRY=
+
</table>
-
|RESOURCES=<span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=2e1n FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=2e1n OCA], [http://www.ebi.ac.uk/pdbsum/2e1n PDBsum], [http://www.rcsb.org/pdb/explore.do?structureId=2e1n RCSB]</span>
+
== Function ==
-
}}
+
[https://www.uniprot.org/uniprot/Q31QG0_SYNE7 Q31QG0_SYNE7]
-
 
+
== Evolutionary Conservation ==
-
'''Crystal structure of the Cyanobacterium circadian clock modifier Pex'''
+
[[Image:Consurf_key_small.gif|200px|right]]
-
 
+
Check<jmol>
-
 
+
<jmolCheckbox>
-
==Overview==
+
<scriptWhenChecked>; select protein; define ~consurf_to_do selected; consurf_initial_scene = true; script "/wiki/ConSurf/e1/2e1n_consurf.spt"</scriptWhenChecked>
-
Circadian clocks are self-sustained biochemical oscillators. The oscillator of cyanobacteria comprises the products of three kai genes (kaiA, kaiB, and kaiC). The autophosphorylation cycle of KaiC oscillates robustly in the cell with a 24-h period and is essential for the basic timing of the cyanobacterial circadian clock. Recently, period extender (pex), mutants of which show a short period phenotype, was classified as a resetting-related gene. In fact, pex mRNA and the pex protein (Pex) increase during the dark period, and a pex mutant subjected to diurnal light-dark cycles shows a 3-h advance in rhythm phase. Here, we report the x-ray crystallographic analysis and biochemical characterization of Pex from cyanobacterium Synechococcus elongatus PCC 7942. The molecule has an (alpha+beta) structure with a winged-helix motif and is indicated to function as a dimer. The subunit arrangement in the dimer is unique and has not been seen in other winged-helix proteins. Electrophoresis mobility shift assay using a 25-base pair complementary oligonucleotide incorporating the kaiA upstream sequence demonstrates that Pex has an affinity for the double-stranded DNA. Furthermore, mutation analysis shows that Pex uses the wing region to recognize the DNA. The in vivo rhythm assay of Pex shows that the constitutive expression of the pex gene harboring the mutation that fails to bind to DNA lacks the period-prolongation activity in the pex-deficient Synechococcus, suggesting that Pex is a DNA-binding transcription factor.
+
<scriptWhenUnchecked>script /wiki/extensions/Proteopedia/spt/initialview01.spt</scriptWhenUnchecked>
-
 
+
<text>to colour the structure by Evolutionary Conservation</text>
-
==About this Structure==
+
</jmolCheckbox>
-
2E1N is a [[Single protein]] structure of sequence from [http://en.wikipedia.org/wiki/Synechococcus_sp. Synechococcus sp.]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2E1N OCA].
+
</jmol>, as determined by [http://consurfdb.tau.ac.il/ ConSurfDB]. You may read the [[Conservation%2C_Evolutionary|explanation]] of the method and the full data available from [http://bental.tau.ac.il/new_ConSurfDB/main_output.php?pdb_ID=2e1n ConSurf].
-
 
+
<div style="clear:both"></div>
-
==Reference==
+
__TOC__
-
Structural and biochemical characterization of a cyanobacterium circadian clock-modifier protein., Arita K, Hashimoto H, Igari K, Akaboshi M, Kutsuna S, Sato M, Shimizu T, J Biol Chem. 2007 Jan 12;282(2):1128-35. Epub 2006 Nov 10. PMID:[http://www.ncbi.nlm.nih.gov/pubmed/17098741 17098741]
+
</StructureSection>
-
[[Category: Single protein]]
+
[[Category: Large Structures]]
-
[[Category: Synechococcus sp.]]
+
[[Category: Synechococcus elongatus PCC 7942 = FACHB-805]]
-
[[Category: Arita, K.]]
+
[[Category: Arita K]]
-
[[Category: Shimizu, T.]]
+
[[Category: Shimizu T]]
-
[[Category: circadian clock]]
+
-
[[Category: dna binding protein]]
+
-
 
+
-
''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Mon Mar 31 02:42:18 2008''
+

Current revision

Crystal structure of the Cyanobacterium circadian clock modifier Pex

PDB ID 2e1n

Drag the structure with the mouse to rotate

Proteopedia Page Contributors and Editors (what is this?)

OCA

Retrieved from "http://52.214.119.220/wiki/index.php/2e1n"
Personal tools