|
|
| (2 intermediate revisions not shown.) |
| Line 1: |
Line 1: |
| | | | |
| | ==Holliday Junction Resolving Enzyme== | | ==Holliday Junction Resolving Enzyme== |
| - | <StructureSection load='1ob9' size='340' side='right' caption='[[1ob9]], [[Resolution|resolution]] 2.00Å' scene=''> | + | <StructureSection load='1ob9' size='340' side='right'caption='[[1ob9]], [[Resolution|resolution]] 2.00Å' scene=''> |
| | == Structural highlights == | | == Structural highlights == |
| - | <table><tr><td colspan='2'>[[1ob9]] is a 1 chain structure with sequence from [http://en.wikipedia.org/wiki/Atcc_35091 Atcc 35091]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1OB9 OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=1OB9 FirstGlance]. <br> | + | <table><tr><td colspan='2'>[[1ob9]] is a 1 chain structure with sequence from [https://en.wikipedia.org/wiki/Saccharolobus_solfataricus Saccharolobus solfataricus]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1OB9 OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=1OB9 FirstGlance]. <br> |
| - | </td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=EDO:1,2-ETHANEDIOL'>EDO</scene>, <scene name='pdbligand=FMT:FORMIC+ACID'>FMT</scene></td></tr> | + | </td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution|Resolution]] 2Å</td></tr> |
| - | <tr id='related'><td class="sblockLbl"><b>[[Related_structure|Related:]]</b></td><td class="sblockDat">[[1ob8|1ob8]]</td></tr> | + | <tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=EDO:1,2-ETHANEDIOL'>EDO</scene>, <scene name='pdbligand=FMT:FORMIC+ACID'>FMT</scene></td></tr> |
| - | <tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=1ob9 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1ob9 OCA], [http://pdbe.org/1ob9 PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=1ob9 RCSB], [http://www.ebi.ac.uk/pdbsum/1ob9 PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=1ob9 ProSAT]</span></td></tr> | + | <tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=1ob9 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1ob9 OCA], [https://pdbe.org/1ob9 PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=1ob9 RCSB], [https://www.ebi.ac.uk/pdbsum/1ob9 PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=1ob9 ProSAT]</span></td></tr> |
| | </table> | | </table> |
| | == Function == | | == Function == |
| - | [[http://www.uniprot.org/uniprot/HJE_SULSO HJE_SULSO]] A structure-specific endonuclease that resolves Holliday junction (HJ) intermediates during genetic recombination. Acts only on 4-way DNA junctions in a sequence non-specific manner; introduces paired nicks in opposing strands 2 bases 3' of the point of strand exchange only on continuous strands of 4-way junction DNA. Cleaves both mobile and immobile junctions.<ref>PMID:10623519</ref> <ref>PMID:10736227</ref> <ref>PMID:15921693</ref> | + | [https://www.uniprot.org/uniprot/HJE_SACS2 HJE_SACS2] A structure-specific endonuclease that resolves Holliday junction (HJ) intermediates during genetic recombination. Acts only on 4-way DNA junctions in a sequence non-specific manner; introduces paired nicks in opposing strands 2 bases 3' of the point of strand exchange only on continuous strands of 4-way junction DNA. Cleaves both mobile and immobile junctions.<ref>PMID:10623519</ref> <ref>PMID:10736227</ref> <ref>PMID:15921693</ref> |
| | == Evolutionary Conservation == | | == Evolutionary Conservation == |
| | [[Image:Consurf_key_small.gif|200px|right]] | | [[Image:Consurf_key_small.gif|200px|right]] |
| Line 29: |
Line 29: |
| | </div> | | </div> |
| | <div class="pdbe-citations 1ob9" style="background-color:#fffaf0;"></div> | | <div class="pdbe-citations 1ob9" style="background-color:#fffaf0;"></div> |
| | + | |
| | + | ==See Also== |
| | + | *[[Resolvase 3D structures|Resolvase 3D structures]] |
| | == References == | | == References == |
| | <references/> | | <references/> |
| | __TOC__ | | __TOC__ |
| | </StructureSection> | | </StructureSection> |
| - | [[Category: Atcc 35091]] | + | [[Category: Large Structures]] |
| - | [[Category: Bond, C S]] | + | [[Category: Saccharolobus solfataricus]] |
| - | [[Category: Middleton, C L]] | + | [[Category: Bond CS]] |
| - | [[Category: Parker, J L]] | + | [[Category: Middleton CL]] |
| - | [[Category: Richard, D J]] | + | [[Category: Parker JL]] |
| - | [[Category: White, M F]] | + | [[Category: Richard DJ]] |
| - | [[Category: Archaea]] | + | [[Category: White MF]] |
| - | [[Category: Enzyme]]
| + | |
| - | [[Category: Holliday junction resolving enzyme]]
| + | |
| - | [[Category: Homologous recombination]]
| + | |
| - | [[Category: Hydrolase]]
| + | |
| - | [[Category: Nuclease]]
| + | |
| - | [[Category: Thermophile]]
| + | |
| Structural highlights
Function
HJE_SACS2 A structure-specific endonuclease that resolves Holliday junction (HJ) intermediates during genetic recombination. Acts only on 4-way DNA junctions in a sequence non-specific manner; introduces paired nicks in opposing strands 2 bases 3' of the point of strand exchange only on continuous strands of 4-way junction DNA. Cleaves both mobile and immobile junctions.[1] [2] [3]
Evolutionary Conservation
Check, as determined by ConSurfDB. You may read the explanation of the method and the full data available from ConSurf.
Publication Abstract from PubMed
Two archaeal Holliday junction resolving enzymes, Holliday junction cleavage (Hjc) and Holliday junction endonuclease (Hje), have been characterized. Both are members of a nuclease superfamily that includes the type II restriction enzymes, although their DNA cleaving activity is highly specific for four-way junction structure and not nucleic acid sequence. Despite 28% sequence identity, Hje and Hjc cleave junctions with distinct cutting patterns--they cut different strands of a four-way junction, at different distances from the junction centre. We report the high-resolution crystal structure of Hje from Sulfolobus solfataricus. The structure provides a basis to explain the differences in substrate specificity of Hje and Hjc, which result from changes in dimer organization, and suggests a viral origin for the Hje gene. Structural and biochemical data support the modelling of an Hje:DNA junction complex, highlighting a flexible loop that interacts intimately with the junction centre. A highly conserved serine residue on this loop is shown to be essential for the enzyme's activity, suggesting a novel variation of the nuclease active site. The loop may act as a conformational switch, ensuring that the active site is completed only on binding a four-way junction, thus explaining the exquisite specificity of these enzymes.
Substrate recognition and catalysis by the Holliday junction resolving enzyme Hje.,Middleton CL, Parker JL, Richard DJ, White MF, Bond CS Nucleic Acids Res. 2004 Oct 12;32(18):5442-51. Print 2004. PMID:15479781[4]
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.
See Also
References
- ↑ Kvaratskhelia M, White MF. An archaeal Holliday junction resolving enzyme from Sulfolobus solfataricus exhibits unique properties. J Mol Biol. 2000 Jan 14;295(2):193-202. PMID:10623519 doi:http://dx.doi.org/10.1006/jmbi.1999.3363
- ↑ Kvaratskhelia M, White MF. Two Holliday junction resolving enzymes in Sulfolobus solfataricus. J Mol Biol. 2000 Apr 7;297(4):923-32. PMID:10736227 doi:http://dx.doi.org/10.1006/jmbi.2000.3624
- ↑ Parker JL, White MF. The endonuclease Hje catalyses rapid, multiple turnover resolution of Holliday junctions. J Mol Biol. 2005 Jul 1;350(1):1-6. PMID:15921693 doi:http://dx.doi.org/10.1016/j.jmb.2005.04.056
- ↑ Middleton CL, Parker JL, Richard DJ, White MF, Bond CS. Substrate recognition and catalysis by the Holliday junction resolving enzyme Hje. Nucleic Acids Res. 2004 Oct 12;32(18):5442-51. Print 2004. PMID:15479781 doi:http://dx.doi.org/32/18/5442
|
