1lp8

<StructureSection load='1lp8' size='340' side='right' caption='[[1lp8]], [[Resolution|resolution]] 1.65&Aring;' scene=''>

<table><tr><td colspan='2'>[[1lp8]] is a 1 chain structure with sequence from [http://en.wikipedia.org/wiki/Carnation Carnation]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1LP8 OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=1LP8 FirstGlance]. <br>

</td></tr><tr id='related'><td class="sblockLbl"><b>[[Related_structure|Related:]]</b></td><td class="sblockDat">[[1lpc|1lpc]], [[1lpd|1lpd]]</td></tr>

<tr id='activity'><td class="sblockLbl"><b>Activity:</b></td><td class="sblockDat"><span class='plainlinks'>[http://en.wikipedia.org/wiki/rRNA_N-glycosylase rRNA N-glycosylase], with EC number [http://www.brenda-enzymes.info/php/result_flat.php4?ecno=3.2.2.22 3.2.2.22] </span></td></tr>

<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=1lp8 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1lp8 OCA], [http://pdbe.org/1lp8 PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=1lp8 RCSB], [http://www.ebi.ac.uk/pdbsum/1lp8 PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=1lp8 ProSAT]</span></td></tr>

[[http://www.uniprot.org/uniprot/RIP0_DIACA RIP0_DIACA]] Single-chain ribosome-inactivating protein.

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== Publication Abstract from PubMed ==

Dianthin antiviral protein (DAP) is a naturally occurring antiviral protein from the leaves of carnation (Dianthus caryophyllus) capable of depurinating HIV-1 RNA and inhibiting HIV-1 replication in human peripheral blood mononuclear cells. Escherichia coli-derived recombinant DAP (rDAP, amino acids 1-254) was purified to homogeneity for structural and functional studies. In the following paper the X-ray crystal structure of rDAP as well as its complexes with cyclic AMP and adenyl-guanosine (ApG) as substrate analogs at 1.7 A resolution are reported. Molecular modeling studies of the interactions of DAP and the structurally similar pokeweed antiviral protein (PAP) with a single-stranded RNA heptamer predicted a more potent anti-HIV activity for rDAP due to its unique surface topology and more favorable charge distribution in its 20 A-long RNA binding active center cleft. In accordance with the predictions of the modeling studies, rDAP was more potent than rPAP in depurinating HIV-1 RNA. To the knowledge of the authors, this is the first structural and functional characterization of recombinant DAP.

 

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== References ==

[[Category: Carnation]]

[[Category: RRNA N-glycosylase]]

[[Category: Kurinov, I V]]

[[Category: Rajamohan, F]]

[[Category: Uckun, F M]]

[[Category: Ribosome inactivating protein]]