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6c66
From Proteopedia
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| - | '''Unreleased structure''' | ||
| - | + | ==CRISPR RNA-guided surveillance complex, pre-nicking== | |
| + | <SX load='6c66' size='340' side='right' viewer='molstar' caption='[[6c66]], [[Resolution|resolution]] 3.66Å' scene=''> | ||
| + | == Structural highlights == | ||
| + | <table><tr><td colspan='2'>[[6c66]] is a 15 chain structure with sequence from [https://en.wikipedia.org/wiki/Thermobifida_fusca Thermobifida fusca] and [https://en.wikipedia.org/wiki/Thermobifida_fusca_YX Thermobifida fusca YX]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=6C66 OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=6C66 FirstGlance]. <br> | ||
| + | </td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">Electron Microscopy, [[Resolution|Resolution]] 3.66Å</td></tr> | ||
| + | <tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=FE:FE+(III)+ION'>FE</scene></td></tr> | ||
| + | <tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=6c66 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=6c66 OCA], [https://pdbe.org/6c66 PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=6c66 RCSB], [https://www.ebi.ac.uk/pdbsum/6c66 PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=6c66 ProSAT]</span></td></tr> | ||
| + | </table> | ||
| + | == Function == | ||
| + | [https://www.uniprot.org/uniprot/Q47PJ0_THEFY Q47PJ0_THEFY] | ||
| + | <div style="background-color:#fffaf0;"> | ||
| + | == Publication Abstract from PubMed == | ||
| + | Type I CRISPR-Cas system features a sequential target-searching and degradation process on double-stranded DNA, by the RNA-guided Cascade complex and the nuclease-helicase fusion enzyme Cas3, respectively. Here we present a 3.7 A resolution cryo-EM structure of the Type I-E Cascade/R-loop/Cas3 complex, poised to initiate DNA degradation. Cas3 distinguishes Cascade conformations and only captures the R-loop forming Cascade, to avoid cleaving partially complementary targets. Its nuclease domain recruits the non-target strand (NTS) DNA at a bulged region for the nicking of single-stranded DNA. An additional 4.7 A resolution cryo-EM structure captures the post-nicking state, in which the severed NTS retracts to the helicase entrance, to be threaded for ATP-dependent processive degradation. These snapshots form the basis for understanding RNA-guided DNA degradation in Type I-E CRISPR-Cas systems. | ||
| - | + | Structure basis for RNA-guided DNA degradation by Cascade and Cas3.,Xiao Y, Luo M, Dolan AE, Liao M, Ke A Science. 2018 Jun 7. pii: science.aat0839. doi: 10.1126/science.aat0839. PMID:29880725<ref>PMID:29880725</ref> | |
| - | + | From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br> | |
| - | [[Category: | + | </div> |
| + | <div class="pdbe-citations 6c66" style="background-color:#fffaf0;"></div> | ||
| + | |||
| + | ==See Also== | ||
| + | *[[CRISPR type I-E (Cascade)|CRISPR type I-E (Cascade)]] | ||
| + | *[[Helicase 3D structures|Helicase 3D structures]] | ||
| + | == References == | ||
| + | <references/> | ||
| + | __TOC__ | ||
| + | </SX> | ||
| + | [[Category: Large Structures]] | ||
| + | [[Category: Thermobifida fusca]] | ||
| + | [[Category: Thermobifida fusca YX]] | ||
| + | [[Category: Ke A]] | ||
| + | [[Category: Liao M]] | ||
| + | [[Category: Luo M]] | ||
| + | [[Category: Xiao Y]] | ||
Current revision
CRISPR RNA-guided surveillance complex, pre-nicking
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