6c8r
From Proteopedia
(Difference between revisions)
| (2 intermediate revisions not shown.) | |||
| Line 1: | Line 1: | ||
==Loganic acid O-methyltransferase complexed with SAH and loganic acid== | ==Loganic acid O-methyltransferase complexed with SAH and loganic acid== | ||
| - | <StructureSection load='6c8r' size='340' side='right' caption='[[6c8r]], [[Resolution|resolution]] 1.95Å' scene=''> | + | <StructureSection load='6c8r' size='340' side='right'caption='[[6c8r]], [[Resolution|resolution]] 1.95Å' scene=''> |
== Structural highlights == | == Structural highlights == | ||
| - | <table><tr><td colspan='2'>[[6c8r]] is a 2 chain structure. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=6C8R OCA]. For a <b>guided tour on the structure components</b> use [ | + | <table><tr><td colspan='2'>[[6c8r]] is a 2 chain structure with sequence from [https://en.wikipedia.org/wiki/Catharanthus_roseus Catharanthus roseus]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=6C8R OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=6C8R FirstGlance]. <br> |
| - | </td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=EQV:Loganic+acid'>EQV</scene>, <scene name='pdbligand=SAH:S-ADENOSYL-L-HOMOCYSTEINE'>SAH</scene></td></tr> | + | </td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution|Resolution]] 1.951Å</td></tr> |
| - | <tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[ | + | <tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=EQV:Loganic+acid'>EQV</scene>, <scene name='pdbligand=SAH:S-ADENOSYL-L-HOMOCYSTEINE'>SAH</scene></td></tr> |
| + | <tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=6c8r FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=6c8r OCA], [https://pdbe.org/6c8r PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=6c8r RCSB], [https://www.ebi.ac.uk/pdbsum/6c8r PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=6c8r ProSAT]</span></td></tr> | ||
</table> | </table> | ||
| + | == Function == | ||
| + | [https://www.uniprot.org/uniprot/LAMT_CATRO LAMT_CATRO] Component of the seco-iridoid and derivatives monoterpenoid indole alkaloids (MIAs, e.g. vinblastine and ajmalicine) biosynthesis pathway. Catalyzes the methylation of loganic acid (6S,7R) to produce loganin (PubMed:29399933, PubMed:18326827, PubMed:24104568). Weak activity with secologanic acid as substrate. Inactive on deoxyloganic, dehydrologanic, epiloganic and loganetic acid (PubMed:18326827).<ref>PMID:18326827</ref> <ref>PMID:24104568</ref> <ref>PMID:29399933</ref> | ||
| + | <div style="background-color:#fffaf0;"> | ||
| + | == Publication Abstract from PubMed == | ||
| + | : Loganin is an iridoid glycoside of interest as both an intermediate in the biosynthesis of indole alkaloids in plants and as a bioactive compound itself. The loganic acid methyltransferase catalyzes the methylation of a monoterpenoid glycoside precursor to produce loganin, and demonstrates stereospecificity for the (6S,7R) substrate. Here, we biochemically characterize this biocatalyst and elucidate the basis for its strict substrate specificity. These studies may help facilitate the design of new classes of monoterpenoid indole alkaloids of pharmaceutical interest. | ||
| + | |||
| + | Loganic Acid Methyltransferase: Insights into the specificity of methylation on an iridoid glycoside.,Petronikolou N, Hollatz A, Schuler MA, Nair SK Chembiochem. 2018 Feb 5. doi: 10.1002/cbic.201700679. PMID:29399933<ref>PMID:29399933</ref> | ||
| + | |||
| + | From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br> | ||
| + | </div> | ||
| + | <div class="pdbe-citations 6c8r" style="background-color:#fffaf0;"></div> | ||
| + | == References == | ||
| + | <references/> | ||
__TOC__ | __TOC__ | ||
</StructureSection> | </StructureSection> | ||
| - | [[Category: | + | [[Category: Catharanthus roseus]] |
| - | [[Category: | + | [[Category: Large Structures]] |
| - | [[Category: | + | [[Category: Nair SK]] |
| - | [[Category: | + | [[Category: Petronikolou N]] |
| - | + | ||
| - | + | ||
Current revision
Loganic acid O-methyltransferase complexed with SAH and loganic acid
| |||||||||||
