6ey5

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==C-terminal part (residues 224-515) of PorM==
==C-terminal part (residues 224-515) of PorM==
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<StructureSection load='6ey5' size='340' side='right' caption='[[6ey5]], [[Resolution|resolution]] 2.85&Aring;' scene=''>
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<StructureSection load='6ey5' size='340' side='right'caption='[[6ey5]], [[Resolution|resolution]] 2.85&Aring;' scene=''>
== Structural highlights ==
== Structural highlights ==
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<table><tr><td colspan='2'>[[6ey5]] is a 2 chain structure. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=6EY5 OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=6EY5 FirstGlance]. <br>
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<table><tr><td colspan='2'>[[6ey5]] is a 2 chain structure with sequence from [https://en.wikipedia.org/wiki/Porphyromonas_gingivalis Porphyromonas gingivalis]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=6EY5 OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=6EY5 FirstGlance]. <br>
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</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=ZN:ZINC+ION'>ZN</scene></td></tr>
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</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution|Resolution]] 2.85&#8491;</td></tr>
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<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=6ey5 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=6ey5 OCA], [http://pdbe.org/6ey5 PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=6ey5 RCSB], [http://www.ebi.ac.uk/pdbsum/6ey5 PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=6ey5 ProSAT]</span></td></tr>
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<tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=ZN:ZINC+ION'>ZN</scene></td></tr>
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<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=6ey5 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=6ey5 OCA], [https://pdbe.org/6ey5 PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=6ey5 RCSB], [https://www.ebi.ac.uk/pdbsum/6ey5 PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=6ey5 ProSAT]</span></td></tr>
</table>
</table>
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== Function ==
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[https://www.uniprot.org/uniprot/B2RLE8_PORG3 B2RLE8_PORG3]
<div style="background-color:#fffaf0;">
<div style="background-color:#fffaf0;">
== Publication Abstract from PubMed ==
== Publication Abstract from PubMed ==
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PorM is a membrane protein involved in the assembly of the type IX secretion system (T9SS) from Porphyromonas gingivalis, a major bacterial pathogen responsible for periodontal disease in humans. The periplasmic domain of PorM was overexpressed in Escherichia coli and purified. A fragment of the purified protein was obtained by limited proteolysis. Crystals of this fragment belonged to the tetragonal space group P4(3)2(1)2. Native and MAD data sets were recorded to 2.85 and 3.1 A resolution, respectively, using synchrotron radiation.
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Type IX secretion system (T9SS), exclusively present in the Bacteroidetes phylum, has been studied mainly in Flavobacterium johnsoniae and Porphyromonas gingivalis. Among the 18 genes, essential for T9SS function, a group of four, porK-N (P. gingivalis) or gldK-N (F. johnsoniae) belongs to a co-transcribed operon that expresses the T9SS core membrane complex. The central component of this complex, PorM (or GldM), is anchored in the inner membrane by a trans-membrane helix and interacts through the outer membrane PorK-N complex. There is a complete lack of available atomic structures for any component of T9SS, including the PorKLMN complex. Here we report the crystal structure of the GldM and PorM periplasmic domains. Dimeric GldM and PorM, each contain four domains of ~180-A length that span most of the periplasmic space. These and previously reported results allow us to propose a model of the T9SS core membrane complex as well as its functional behavior.
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Crystallization and preliminary X-ray analysis of the C-terminal fragment of PorM, a subunit of the Porphyromonas gingivalis type IX secretion system.,Stathopulos J, Cambillau C, Cascales E, Roussel A, Leone P Acta Crystallogr F Struct Biol Commun. 2015 Jan 1;71(Pt 1):71-4. doi:, 10.1107/S2053230X1402559X. Epub 2015 Jan 1. PMID:25615973<ref>PMID:25615973</ref>
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Type IX secretion system PorM and gliding machinery GldM form arches spanning the periplasmic space.,Leone P, Roche J, Vincent MS, Tran QH, Desmyter A, Cascales E, Kellenberger C, Cambillau C, Roussel A Nat Commun. 2018 Jan 30;9(1):429. doi: 10.1038/s41467-017-02784-7. PMID:29382829<ref>PMID:29382829</ref>
From MEDLINE&reg;/PubMed&reg;, a database of the U.S. National Library of Medicine.<br>
From MEDLINE&reg;/PubMed&reg;, a database of the U.S. National Library of Medicine.<br>
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__TOC__
__TOC__
</StructureSection>
</StructureSection>
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[[Category: Cambillau, C]]
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[[Category: Large Structures]]
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[[Category: Leone, P]]
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[[Category: Porphyromonas gingivalis]]
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[[Category: Roussel, A]]
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[[Category: Cambillau C]]
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[[Category: Nanobody]]
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[[Category: Leone P]]
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[[Category: Protein transport]]
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[[Category: Roussel A]]

Current revision

C-terminal part (residues 224-515) of PorM

PDB ID 6ey5

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