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| - | [[Image:2ebf.jpg|left|200px]] | |
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| - | {{Structure
| + | ==Crystal structures reveal a thiol-protease like catalytic triad in the C-terminal region of Pasteurella multocida toxin== |
| - | |PDB= 2ebf |SIZE=350|CAPTION= <scene name='initialview01'>2ebf</scene>, resolution 1.90Å
| + | <StructureSection load='2ebf' size='340' side='right'caption='[[2ebf]]' scene=''> |
| - | |SITE=
| + | == Structural highlights == |
| - | |LIGAND= <scene name='pdbligand=2PO:PHOSPHONATE'>2PO</scene>, <scene name='pdbligand=TRE:TREHALOSE'>TRE</scene>
| + | <table><tr><td colspan='2'>Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2EBF OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=2EBF FirstGlance]. <br> |
| - | |ACTIVITY=
| + | </td></tr><tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=2ebf FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=2ebf OCA], [https://pdbe.org/2ebf PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=2ebf RCSB], [https://www.ebi.ac.uk/pdbsum/2ebf PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=2ebf ProSAT]</span></td></tr> |
| - | |GENE= toxA ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=747 Pasteurella multocida])
| + | </table> |
| - | |DOMAIN=
| + | == Evolutionary Conservation == |
| - | |RELATEDENTRY=[[2ebh|2EBH]], [[2ec5|2EC5]]
| + | [[Image:Consurf_key_small.gif|200px|right]] |
| - | |RESOURCES=<span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=2ebf FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=2ebf OCA], [http://www.ebi.ac.uk/pdbsum/2ebf PDBsum], [http://www.rcsb.org/pdb/explore.do?structureId=2ebf RCSB]</span>
| + | Check<jmol> |
| - | }}
| + | <jmolCheckbox> |
| - | | + | <scriptWhenChecked>; select protein; define ~consurf_to_do selected; consurf_initial_scene = true; script "/wiki/ConSurf/eb/2ebf_consurf.spt"</scriptWhenChecked> |
| - | '''Crystal structures reveal a thiol-protease like catalytic triad in the C-terminal region of Pasteurella multocida toxin'''
| + | <scriptWhenUnchecked>script /wiki/extensions/Proteopedia/spt/initialview01.spt</scriptWhenUnchecked> |
| - | | + | <text>to colour the structure by Evolutionary Conservation</text> |
| - | | + | </jmolCheckbox> |
| - | ==Overview==
| + | </jmol>, as determined by [http://consurfdb.tau.ac.il/ ConSurfDB]. You may read the [[Conservation%2C_Evolutionary|explanation]] of the method and the full data available from [http://bental.tau.ac.il/new_ConSurfDB/main_output.php?pdb_ID=2ebf ConSurf]. |
| - | Pasteurella multocida toxin (PMT), one of the virulence factors produced by the bacteria, exerts its toxicity by up-regulating various signaling cascades downstream of the heterotrimeric GTPases Gq and G12/13 in an unknown fashion. Here, we present the crystal structure of the C-terminal region (residues 575-1,285) of PMT, which carries an intracellularly active moiety. The overall structure of C-terminal region of PMT displays a Trojan horse-like shape, composed of three domains with a "feet"-,"body"-, and "head"-type arrangement, which were designated C1, C2, and C3 from the N to the C terminus, respectively. The C1 domain, showing marked similarity in steric structure to the N-terminal domain of Clostridium difficile toxin B, was found to lead the toxin molecule to the plasma membrane. The C3 domain possesses the Cys-His-Asp catalytic triad that is organized only when the Cys is released from a disulfide bond. The steric alignment of the triad corresponded well to that of papain or other enzymes carrying Cys-His-Asp. PMT toxicities on target cells were completely abrogated when one of the amino acids constituting the triad was mutated. Our results indicate that PMT is an enzyme toxin carrying the cysteine protease-like catalytic triad dependent on the redox state and functions on the cytoplasmic face of the plasma membrane of target cells.
| + | <div style="clear:both"></div> |
| - | | + | __TOC__ |
| - | ==About this Structure== | + | </StructureSection> |
| - | 2EBF is a [[Single protein]] structure of sequence from [http://en.wikipedia.org/wiki/Pasteurella_multocida Pasteurella multocida]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2EBF OCA].
| + | [[Category: Large Structures]] |
| - | | + | [[Category: Horiguchi Y]] |
| - | ==Reference==
| + | [[Category: Kamitani S]] |
| - | Crystal structures reveal a thiol protease-like catalytic triad in the C-terminal region of Pasteurella multocida toxin., Kitadokoro K, Kamitani S, Miyazawa M, Hanajima-Ozawa M, Fukui A, Miyake M, Horiguchi Y, Proc Natl Acad Sci U S A. 2007 Mar 20;104(12):5139-44. Epub 2007 Mar 14. PMID:[http://www.ncbi.nlm.nih.gov/pubmed/17360394 17360394]
| + | [[Category: Kitadokoro K]] |
| - | [[Category: Pasteurella multocida]]
| + | |
| - | [[Category: Single protein]] | + | |
| - | [[Category: Horiguchi, Y.]] | + | |
| - | [[Category: Kamitani, S.]] | + | |
| - | [[Category: Kitadokoro, K.]] | + | |
| - | [[Category: pasteurella multocida toxin]]
| + | |
| - | [[Category: trojan horse-like fold]]
| + | |
| - | | + | |
| - | ''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Mon Mar 31 02:46:34 2008''
| + | |
