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| | ==Structure of the GCM domain bound to DNA== | | ==Structure of the GCM domain bound to DNA== |
| - | <StructureSection load='1odh' size='340' side='right' caption='[[1odh]], [[Resolution|resolution]] 2.85Å' scene=''> | + | <StructureSection load='1odh' size='340' side='right'caption='[[1odh]], [[Resolution|resolution]] 2.85Å' scene=''> |
| | == Structural highlights == | | == Structural highlights == |
| - | <table><tr><td colspan='2'>[[1odh]] is a 3 chain structure with sequence from [http://en.wikipedia.org/wiki/Lk3_transgenic_mice Lk3 transgenic mice]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1ODH OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=1ODH FirstGlance]. <br> | + | <table><tr><td colspan='2'>[[1odh]] is a 3 chain structure with sequence from [https://en.wikipedia.org/wiki/Mus_musculus Mus musculus]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1ODH OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=1ODH FirstGlance]. <br> |
| - | </td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=ZN:ZINC+ION'>ZN</scene></td></tr> | + | </td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution|Resolution]] 2.85Å</td></tr> |
| - | <tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=1odh FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1odh OCA], [http://pdbe.org/1odh PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=1odh RCSB], [http://www.ebi.ac.uk/pdbsum/1odh PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=1odh ProSAT]</span></td></tr> | + | <tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=ZN:ZINC+ION'>ZN</scene></td></tr> |
| | + | <tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=1odh FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1odh OCA], [https://pdbe.org/1odh PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=1odh RCSB], [https://www.ebi.ac.uk/pdbsum/1odh PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=1odh ProSAT]</span></td></tr> |
| | </table> | | </table> |
| | == Function == | | == Function == |
| - | [[http://www.uniprot.org/uniprot/GCM1_MOUSE GCM1_MOUSE]] Transcription factor that is necessary for placental development (By similarity).<ref>PMID:8962155</ref> | + | [https://www.uniprot.org/uniprot/GCM1_MOUSE GCM1_MOUSE] Transcription factor that is necessary for placental development (By similarity).<ref>PMID:8962155</ref> |
| | == Evolutionary Conservation == | | == Evolutionary Conservation == |
| | [[Image:Consurf_key_small.gif|200px|right]] | | [[Image:Consurf_key_small.gif|200px|right]] |
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| | __TOC__ | | __TOC__ |
| | </StructureSection> | | </StructureSection> |
| - | [[Category: Lk3 transgenic mice]] | + | [[Category: Large Structures]] |
| - | [[Category: Cohen, S X]] | + | [[Category: Mus musculus]] |
| - | [[Category: Muller, C W]] | + | [[Category: Cohen SX]] |
| - | [[Category: Dna-binding domain]] | + | [[Category: Muller CW]] |
| - | [[Category: Protein-dna complex]]
| + | |
| - | [[Category: Transcription factor]]
| + | |
| - | [[Category: Transcription factor-dna complex]]
| + | |
| - | [[Category: Transcription factor/dna]]
| + | |
| Structural highlights
Function
GCM1_MOUSE Transcription factor that is necessary for placental development (By similarity).[1]
Evolutionary Conservation
Check, as determined by ConSurfDB. You may read the explanation of the method and the full data available from ConSurf.
Publication Abstract from PubMed
Glia cell missing (GCM) transcription factors form a small family of transcriptional regulators in metazoans. The prototypical Drosophila GCM protein directs the differentiation of neuron precursor cells into glia cells, whereas mammalian GCM proteins are involved in placenta and parathyroid development. GCM proteins share a highly conserved 150 amino acid residue region responsible for DNA binding, known as the GCM domain. Here we present the crystal structure of the GCM domain from murine GCMa bound to its octameric DNA target site at 2.85 A resolution. The GCM domain exhibits a novel fold consisting of two domains tethered together by one of two structural Zn ions. We observe the novel use of a beta-sheet in DNA recognition, whereby a five- stranded beta-sheet protrudes into the major groove perpendicular to the DNA axis. The structure combined with mutational analysis of the target site and of DNA-contacting residues provides insight into DNA recognition by this new type of Zn-containing DNA-binding domain.
Structure of the GCM domain-DNA complex: a DNA-binding domain with a novel fold and mode of target site recognition.,Cohen SX, Moulin M, Hashemolhosseini S, Kilian K, Wegner M, Muller CW EMBO J. 2003 Apr 15;22(8):1835-45. PMID:12682016[2]
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.
References
- ↑ Akiyama Y, Hosoya T, Poole AM, Hotta Y. The gcm-motif: a novel DNA-binding motif conserved in Drosophila and mammals. Proc Natl Acad Sci U S A. 1996 Dec 10;93(25):14912-6. PMID:8962155
- ↑ Cohen SX, Moulin M, Hashemolhosseini S, Kilian K, Wegner M, Muller CW. Structure of the GCM domain-DNA complex: a DNA-binding domain with a novel fold and mode of target site recognition. EMBO J. 2003 Apr 15;22(8):1835-45. PMID:12682016 doi:http://dx.doi.org/10.1093/emboj/cdg182
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