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| | ==The crystal structure of C3stau2 from S. aureus== | | ==The crystal structure of C3stau2 from S. aureus== |
| - | <StructureSection load='1ojq' size='340' side='right' caption='[[1ojq]], [[Resolution|resolution]] 1.68Å' scene=''> | + | <StructureSection load='1ojq' size='340' side='right'caption='[[1ojq]], [[Resolution|resolution]] 1.68Å' scene=''> |
| | == Structural highlights == | | == Structural highlights == |
| - | <table><tr><td colspan='2'>[[1ojq]] is a 1 chain structure with sequence from [http://en.wikipedia.org/wiki/"micrococcus_aureus"_(rosenbach_1884)_zopf_1885 "micrococcus aureus" (rosenbach 1884) zopf 1885]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1OJQ OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=1OJQ FirstGlance]. <br> | + | <table><tr><td colspan='2'>[[1ojq]] is a 1 chain structure with sequence from [https://en.wikipedia.org/wiki/Staphylococcus_aureus Staphylococcus aureus]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1OJQ OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=1OJQ FirstGlance]. <br> |
| - | </td></tr><tr id='related'><td class="sblockLbl"><b>[[Related_structure|Related:]]</b></td><td class="sblockDat">[[1gxy|1gxy]], [[1gxz|1gxz]], [[1gze|1gze]], [[1gzf|1gzf]], [[1gy0|1gy0]], [[1og1|1og1]], [[1og3|1og3]], [[1og4|1og4]], [[1ojz|1ojz]]</td></tr> | + | </td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution|Resolution]] 1.68Å</td></tr> |
| - | <tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=1ojq FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1ojq OCA], [http://pdbe.org/1ojq PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=1ojq RCSB], [http://www.ebi.ac.uk/pdbsum/1ojq PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=1ojq ProSAT]</span></td></tr> | + | <tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=1ojq FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1ojq OCA], [https://pdbe.org/1ojq PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=1ojq RCSB], [https://www.ebi.ac.uk/pdbsum/1ojq PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=1ojq ProSAT]</span></td></tr> |
| | </table> | | </table> |
| | + | == Function == |
| | + | [https://www.uniprot.org/uniprot/Q9ADS9_STAAU Q9ADS9_STAAU] |
| | == Evolutionary Conservation == | | == Evolutionary Conservation == |
| | [[Image:Consurf_key_small.gif|200px|right]] | | [[Image:Consurf_key_small.gif|200px|right]] |
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| | __TOC__ | | __TOC__ |
| | </StructureSection> | | </StructureSection> |
| - | [[Category: Acharya, K R]] | + | [[Category: Large Structures]] |
| - | [[Category: Ayriss, J]] | + | [[Category: Staphylococcus aureus]] |
| - | [[Category: Evans, H R]] | + | [[Category: Acharya KR]] |
| - | [[Category: Holloway, D E]] | + | [[Category: Ayriss J]] |
| - | [[Category: Shone, C C]] | + | [[Category: Evans HR]] |
| - | [[Category: Sutton, J M]] | + | [[Category: Holloway DE]] |
| - | [[Category: Adp-ribosyltransferase]] | + | [[Category: Shone CC]] |
| - | [[Category: Transferase]] | + | [[Category: Sutton JM]] |
| Structural highlights
Function
Q9ADS9_STAAU
Evolutionary Conservation
Check, as determined by ConSurfDB. You may read the explanation of the method and the full data available from ConSurf.
Publication Abstract from PubMed
The C3stau2 exoenzyme from Staphylococcus aureus is a C3-like ADP-ribosyltransferase that ADP-ribosylates not only RhoA-C but also RhoE/Rnd3. In this study we have crystallized and determined the structure of C3stau2 in both its native form and in complex with NAD at 1.68- and 2.02-A resolutions, respectively. The topology of C3stau2 is similar to that of C3bot1 from Clostridium botulinum (with which it shares 35% amino acid sequence identity) with the addition of two extra helices after strand beta1. The native structure also features a novel orientation of the catalytic ARTT loop, which approximates the conformation seen for the "NAD bound" form of C3bot1. C3stau2 orients NAD similarly to C3bot1, and on binding NAD, C3stau2 undergoes a clasping motion and a rearrangement of the phosphate-nicotinamide binding loop, enclosing the NAD in the binding site. Comparison of these structures with those of C3bot1 and related toxins reveals a degree of divergence in the interactions with the adenine moiety among the ADP-ribosylating toxins that contrasts with the more conserved interactions with the nicotinamide. Comparison with C3bot1 gives some insight into the different protein substrate specificities of these enzymes.
The crystal structure of C3stau2 from Staphylococcus aureus and its complex with NAD.,Evans HR, Sutton JM, Holloway DE, Ayriss J, Shone CC, Acharya KR J Biol Chem. 2003 Nov 14;278(46):45924-30. Epub 2003 Aug 21. PMID:12933793[1]
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.
References
- ↑ Evans HR, Sutton JM, Holloway DE, Ayriss J, Shone CC, Acharya KR. The crystal structure of C3stau2 from Staphylococcus aureus and its complex with NAD. J Biol Chem. 2003 Nov 14;278(46):45924-30. Epub 2003 Aug 21. PMID:12933793 doi:http://dx.doi.org/10.1074/jbc.M307719200
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