1nt4

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==Crystal structure of Escherichia coli periplasmic glucose-1-phosphatase H18A mutant complexed with glucose-1-phosphate==
==Crystal structure of Escherichia coli periplasmic glucose-1-phosphatase H18A mutant complexed with glucose-1-phosphate==
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<StructureSection load='1nt4' size='340' side='right' caption='[[1nt4]], [[Resolution|resolution]] 2.40&Aring;' scene=''>
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<StructureSection load='1nt4' size='340' side='right'caption='[[1nt4]], [[Resolution|resolution]] 2.40&Aring;' scene=''>
== Structural highlights ==
== Structural highlights ==
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<table><tr><td colspan='2'>[[1nt4]] is a 2 chain structure with sequence from [http://en.wikipedia.org/wiki/"bacillus_coli"_migula_1895 "bacillus coli" migula 1895]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1NT4 OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=1NT4 FirstGlance]. <br>
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<table><tr><td colspan='2'>[[1nt4]] is a 2 chain structure with sequence from [https://en.wikipedia.org/wiki/Escherichia_coli Escherichia coli]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1NT4 OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=1NT4 FirstGlance]. <br>
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</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=G1P:ALPHA-D-GLUCOSE-1-PHOSPHATE'>G1P</scene></td></tr>
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</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution|Resolution]] 2.4&#8491;</td></tr>
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<tr id='gene'><td class="sblockLbl"><b>[[Gene|Gene:]]</b></td><td class="sblockDat">AGP OR B1002 ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=562 "Bacillus coli" Migula 1895])</td></tr>
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<tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=XGP:1-O-PHOSPHONO-BETA-D-GLUCOPYRANOSE'>XGP</scene></td></tr>
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<tr id='activity'><td class="sblockLbl"><b>Activity:</b></td><td class="sblockDat"><span class='plainlinks'>[http://en.wikipedia.org/wiki/Glucose-1-phosphatase Glucose-1-phosphatase], with EC number [http://www.brenda-enzymes.info/php/result_flat.php4?ecno=3.1.3.10 3.1.3.10] </span></td></tr>
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<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=1nt4 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1nt4 OCA], [https://pdbe.org/1nt4 PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=1nt4 RCSB], [https://www.ebi.ac.uk/pdbsum/1nt4 PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=1nt4 ProSAT], [https://www.topsan.org/Proteins/BSGI/1nt4 TOPSAN]</span></td></tr>
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<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=1nt4 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1nt4 OCA], [http://pdbe.org/1nt4 PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=1nt4 RCSB], [http://www.ebi.ac.uk/pdbsum/1nt4 PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=1nt4 ProSAT], [http://www.topsan.org/Proteins/BSGI/1nt4 TOPSAN]</span></td></tr>
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</table>
</table>
== Function ==
== Function ==
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[[http://www.uniprot.org/uniprot/AGP_ECOLI AGP_ECOLI]] Absolutely required for the growth of E.coli in a high-phosphate medium containing G-1-P as the sole carbon source.
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[https://www.uniprot.org/uniprot/AGP_ECOLI AGP_ECOLI] Absolutely required for the growth of E.coli in a high-phosphate medium containing G-1-P as the sole carbon source.
== Evolutionary Conservation ==
== Evolutionary Conservation ==
[[Image:Consurf_key_small.gif|200px|right]]
[[Image:Consurf_key_small.gif|200px|right]]
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<jmolCheckbox>
<jmolCheckbox>
<scriptWhenChecked>; select protein; define ~consurf_to_do selected; consurf_initial_scene = true; script "/wiki/ConSurf/nt/1nt4_consurf.spt"</scriptWhenChecked>
<scriptWhenChecked>; select protein; define ~consurf_to_do selected; consurf_initial_scene = true; script "/wiki/ConSurf/nt/1nt4_consurf.spt"</scriptWhenChecked>
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<scriptWhenUnchecked>script /wiki/extensions/Proteopedia/spt/initialview01.spt</scriptWhenUnchecked>
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<scriptWhenUnchecked>script /wiki/extensions/Proteopedia/spt/initialview03.spt</scriptWhenUnchecked>
<text>to colour the structure by Evolutionary Conservation</text>
<text>to colour the structure by Evolutionary Conservation</text>
</jmolCheckbox>
</jmolCheckbox>
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__TOC__
__TOC__
</StructureSection>
</StructureSection>
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[[Category: Bacillus coli migula 1895]]
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[[Category: Escherichia coli]]
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[[Category: Glucose-1-phosphatase]]
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[[Category: Large Structures]]
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[[Category: Structural genomic]]
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[[Category: Cottrill MA]]
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[[Category: Cottrill, M A]]
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[[Category: Forsberg CW]]
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[[Category: Forsberg, C W]]
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[[Category: Jia Z]]
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[[Category: Jia, Z]]
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[[Category: Lee DC]]
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[[Category: Lee, D C]]
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[[Category: Alpha domain]]
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[[Category: Alpha-beta domain]]
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[[Category: Bsgi]]
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[[Category: Enzyme-substrate complex]]
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[[Category: Hydrolase]]
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[[Category: Occluded active site]]
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Current revision

Crystal structure of Escherichia coli periplasmic glucose-1-phosphatase H18A mutant complexed with glucose-1-phosphate

PDB ID 1nt4

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