1ogk
From Proteopedia
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==The crystal structure of Trypanosoma cruzi dUTPase in complex with dUDP== | ==The crystal structure of Trypanosoma cruzi dUTPase in complex with dUDP== | ||
| - | <StructureSection load='1ogk' size='340' side='right' caption='[[1ogk]], [[Resolution|resolution]] 2.85Å' scene=''> | + | <StructureSection load='1ogk' size='340' side='right'caption='[[1ogk]], [[Resolution|resolution]] 2.85Å' scene=''> |
== Structural highlights == | == Structural highlights == | ||
| - | <table><tr><td colspan='2'>[[1ogk]] is a 4 chain structure with sequence from [ | + | <table><tr><td colspan='2'>[[1ogk]] is a 4 chain structure with sequence from [https://en.wikipedia.org/wiki/Trypanosoma_cruzi Trypanosoma cruzi]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1OGK OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=1OGK FirstGlance]. <br> |
| - | </td></tr><tr id=' | + | </td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution|Resolution]] 2.85Å</td></tr> |
| - | <tr id=' | + | <tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=DUD:DEOXYURIDINE-5-DIPHOSPHATE'>DUD</scene></td></tr> |
| - | + | <tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=1ogk FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1ogk OCA], [https://pdbe.org/1ogk PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=1ogk RCSB], [https://www.ebi.ac.uk/pdbsum/1ogk PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=1ogk ProSAT]</span></td></tr> | |
| - | <tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[ | + | |
</table> | </table> | ||
| + | == Function == | ||
| + | [https://www.uniprot.org/uniprot/O15923_TRYCR O15923_TRYCR] | ||
== Evolutionary Conservation == | == Evolutionary Conservation == | ||
[[Image:Consurf_key_small.gif|200px|right]] | [[Image:Consurf_key_small.gif|200px|right]] | ||
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</jmol>, as determined by [http://consurfdb.tau.ac.il/ ConSurfDB]. You may read the [[Conservation%2C_Evolutionary|explanation]] of the method and the full data available from [http://bental.tau.ac.il/new_ConSurfDB/main_output.php?pdb_ID=1ogk ConSurf]. | </jmol>, as determined by [http://consurfdb.tau.ac.il/ ConSurfDB]. You may read the [[Conservation%2C_Evolutionary|explanation]] of the method and the full data available from [http://bental.tau.ac.il/new_ConSurfDB/main_output.php?pdb_ID=1ogk ConSurf]. | ||
<div style="clear:both"></div> | <div style="clear:both"></div> | ||
| - | <div style="background-color:#fffaf0;"> | ||
| - | == Publication Abstract from PubMed == | ||
| - | dUTPase is an essential enzyme involved with nucleotide metabolism and replication. We report here the X-ray structure of Trypanosoma cruzi dUTPase in its native conformation and as a complex with dUDP. These reveal a novel protein fold that displays no structural similarities to previously described dUTPases. The molecular unit is a dimer with two active sites. Nucleotide binding promotes extensive structural rearrangements, secondary structure remodeling, and rigid body displacements of 20 A or more, which effectively bury the substrate within the enzyme core for the purpose of hydrolysis. The molecular complex is a trapped enzyme-substrate arrangement which clearly demonstrates structure-induced specificity and catalytic potential. This enzyme is a novel dUTPase and therefore a potential drug target in the treatment of Chagas' disease. | ||
| - | + | ==See Also== | |
| - | + | *[[DUTPase 3D structures|DUTPase 3D structures]] | |
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| - | == | + | |
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__TOC__ | __TOC__ | ||
</StructureSection> | </StructureSection> | ||
| - | [[Category: | + | [[Category: Large Structures]] |
| - | [[Category: | + | [[Category: Trypanosoma cruzi]] |
| - | [[Category: Bernier-Villamor | + | [[Category: Bernier-Villamor V]] |
| - | [[Category: Dodson | + | [[Category: Dodson EJ]] |
| - | [[Category: Gonzalez-Pacanowska | + | [[Category: Gonzalez-Pacanowska D]] |
| - | [[Category: Harkiolaki | + | [[Category: Harkiolaki M]] |
| - | [[Category: Turkenburg | + | [[Category: Turkenburg JP]] |
| - | [[Category: Wilson | + | [[Category: Wilson KS]] |
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Current revision
The crystal structure of Trypanosoma cruzi dUTPase in complex with dUDP
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