1uup

From Proteopedia

(Difference between revisions)
Jump to: navigation, search
Current revision (08:52, 6 November 2024) (edit) (undo)
 
(18 intermediate revisions not shown.)
Line 1: Line 1:
-
[[Image:1uup.gif|left|200px]]<br />
 
-
<applet load="1uup" size="450" color="white" frame="true" align="right" spinBox="true"
 
-
caption="1uup, resolution 2.6&Aring;" />
 
-
'''CRYSTAL STRUCTURE OF A DIMERIC FORM OF STREPTOCOCCAL PYROGENIC EXOTOXIN A (SPEA1).'''<br />
 
-
==Overview==
+
==Crystal Structure of A Dimeric Form of Streptococcal Pyrogenic Exotoxin A (SpeA1).==
-
Streptococcal pyrogenic exotoxin A (SpeA1) is a bacterial superantigen, associated with scarlet fever and streptococcal toxic shock syndrome, (STSS). SpeA1 is found in both monomeric and dimeric forms, and previous, work suggested that the dimer results from an intermolecular disulfide, bond between the cysteines at positions 90 of each monomer. Here, we, present the crystal structure of the dimeric form of SpeA1. The toxin, crystallizes in the orthorhombic space group P212121, with two dimers in, the crystallographic asymmetric unit. The final structure has a, crystallographic R-factor of 21.52% for 7248 protein atoms, 136 water, molecules, and 4 zinc atoms (one zinc atom per molecule). The implications, of SpeA1 dimer on MHC class II and T-cell receptor recognition are, discussed.
+
<StructureSection load='1uup' size='340' side='right'caption='[[1uup]], [[Resolution|resolution]] 2.60&Aring;' scene=''>
 +
== Structural highlights ==
 +
<table><tr><td colspan='2'>[[1uup]] is a 4 chain structure with sequence from [https://en.wikipedia.org/wiki/Streptococcus_pyogenes Streptococcus pyogenes]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1UUP OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=1UUP FirstGlance]. <br>
 +
</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution|Resolution]] 2.6&#8491;</td></tr>
 +
<tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=ZN:ZINC+ION'>ZN</scene></td></tr>
 +
<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=1uup FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1uup OCA], [https://pdbe.org/1uup PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=1uup RCSB], [https://www.ebi.ac.uk/pdbsum/1uup PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=1uup ProSAT]</span></td></tr>
 +
</table>
 +
== Function ==
 +
[https://www.uniprot.org/uniprot/SPEA_STRPY SPEA_STRPY] Causative agent of the symptoms associated with scarlet fever, have been associated with streptococcal toxic shock-like disease and may play a role in the early events of rheumatic fever.
 +
== Evolutionary Conservation ==
 +
[[Image:Consurf_key_small.gif|200px|right]]
 +
Check<jmol>
 +
<jmolCheckbox>
 +
<scriptWhenChecked>; select protein; define ~consurf_to_do selected; consurf_initial_scene = true; script "/wiki/ConSurf/uu/1uup_consurf.spt"</scriptWhenChecked>
 +
<scriptWhenUnchecked>script /wiki/extensions/Proteopedia/spt/initialview03.spt</scriptWhenUnchecked>
 +
<text>to colour the structure by Evolutionary Conservation</text>
 +
</jmolCheckbox>
 +
</jmol>, as determined by [http://consurfdb.tau.ac.il/ ConSurfDB]. You may read the [[Conservation%2C_Evolutionary|explanation]] of the method and the full data available from [http://bental.tau.ac.il/new_ConSurfDB/main_output.php?pdb_ID=1uup ConSurf].
 +
<div style="clear:both"></div>
 +
<div style="background-color:#fffaf0;">
 +
== Publication Abstract from PubMed ==
 +
Streptococcal pyrogenic exotoxin A (SpeA1) is a bacterial superantigen associated with scarlet fever and streptococcal toxic shock syndrome (STSS). SpeA1 is found in both monomeric and dimeric forms, and previous work suggested that the dimer results from an intermolecular disulfide bond between the cysteines at positions 90 of each monomer. Here, we present the crystal structure of the dimeric form of SpeA1. The toxin crystallizes in the orthorhombic space group P212121, with two dimers in the crystallographic asymmetric unit. The final structure has a crystallographic R-factor of 21.52% for 7248 protein atoms, 136 water molecules, and 4 zinc atoms (one zinc atom per molecule). The implications of SpeA1 dimer on MHC class II and T-cell receptor recognition are discussed.
-
==About this Structure==
+
Crystal structure of a dimeric form of streptococcal pyrogenic exotoxin A (SpeA1).,Baker MD, Gendlina I, Collins CM, Acharya KR Protein Sci. 2004 Sep;13(9):2285-90. Epub 2004 Aug 4. PMID:15295110<ref>PMID:15295110</ref>
-
1UUP is a [http://en.wikipedia.org/wiki/Single_protein Single protein] structure of sequence from [http://en.wikipedia.org/wiki/Streptococcus_pyogenes Streptococcus pyogenes] with ZN as [http://en.wikipedia.org/wiki/ligand ligand]. Structure known Active Site: ZN1. Full crystallographic information is available from [http://ispc.weizmann.ac.il/oca-bin/ocashort?id=1UUP OCA].
+
-
==Reference==
+
From MEDLINE&reg;/PubMed&reg;, a database of the U.S. National Library of Medicine.<br>
-
Crystal structure of a dimeric form of streptococcal pyrogenic exotoxin A (SpeA1)., Baker MD, Gendlina I, Collins CM, Acharya KR, Protein Sci. 2004 Sep;13(9):2285-90. Epub 2004 Aug 4. PMID:[http://ispc.weizmann.ac.il//pmbin/getpm?pmid=15295110 15295110]
+
</div>
-
[[Category: Single protein]]
+
<div class="pdbe-citations 1uup" style="background-color:#fffaf0;"></div>
-
[[Category: Streptococcus pyogenes]]
+
-
[[Category: Acharya, K.R.]]
+
-
[[Category: Baker, M.D.]]
+
-
[[Category: Collins, C.M.]]
+
-
[[Category: Gendlina, I.]]
+
-
[[Category: ZN]]
+
-
[[Category: dimer]]
+
-
[[Category: exotoxin]]
+
-
[[Category: molecular recognition]]
+
-
[[Category: superantigen]]
+
-
[[Category: toxin]]
+
-
[[Category: x-ray crystallography]]
+
-
[[Category: zinc binding]]
+
-
''Page seeded by [http://ispc.weizmann.ac.il/oca OCA ] on Mon Nov 5 17:12:20 2007''
+
==See Also==
 +
*[[Exotoxin 3D structures|Exotoxin 3D structures]]
 +
== References ==
 +
<references/>
 +
__TOC__
 +
</StructureSection>
 +
[[Category: Large Structures]]
 +
[[Category: Streptococcus pyogenes]]
 +
[[Category: Acharya KR]]
 +
[[Category: Baker MD]]
 +
[[Category: Collins CM]]
 +
[[Category: Gendlina I]]

Current revision

Crystal Structure of A Dimeric Form of Streptococcal Pyrogenic Exotoxin A (SpeA1).

PDB ID 1uup

Drag the structure with the mouse to rotate

Proteopedia Page Contributors and Editors (what is this?)

OCA

Retrieved from "http://52.214.119.220/wiki/index.php/1uup"
Personal tools