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==Luciferase== | ==Luciferase== | ||
<StructureSection load='2d1s' size='340' side='right' caption='Protein structure of luciferase' scene=''> | <StructureSection load='2d1s' size='340' side='right' caption='Protein structure of luciferase' scene=''> | ||
| - | This is a default text for your page ''''''. Click above on '''edit this page''' to modify. Be careful with the < and > signs. | ||
| - | You may include any references to papers as in: the use of JSmol in Proteopedia <ref>DOI 10.1002/ijch.201300024</ref> or to the article describing Jmol <ref>PMID:21638687</ref> to the rescue. | ||
| - | <scene name='77/777750/Colored_secondary_structure/2'>TextToBeDisplayed</scene> | ||
| - | <scene name='77/777750/Critical_amino_acid/2'>Text To Be Displayed</scene> | ||
| - | == | + | ==Organism of Origin== |
| + | Luciferase is an eukaryotic enzyme originally found in fireflies. | ||
| - | == | + | ==Protein Structure== |
| + | The total number of amino acids in the protein is 548. The different <scene name='77/777750/Colored_secondary_structure/2'>secondary structures</scene> include alpha helices (seen in pink) and beta sheets (seen in gold). There are a mix of hydrophobic and hydrophilic amino acids that impact protein function. The color emitted by the enzyme is <scene name='77/777750/Critical_amino_acid/2'>green</scene> in the wild type and <scene name='77/777750/Mutant_luciferase/1'>red</scene> in the mutant due to changes in protein folding. | ||
| - | == | + | ==Function== |
| + | Luciferase catabolizes luciferin, which forms a bio-luminescent product. This reaction consumes ATP and oxygen. Luciferase is an oxidoreducase. | ||
| + | |||
| + | ==Significance== | ||
| + | Luciferase is an enzyme commonly used in imaging in the biotechnology industry. A cell line of interest can be genetically engineered to express this enzyme by using a viral vector. Once the model organism has been inoculated with the cell line of interest, luciferin can be administer to track localization and growth. If that cell line of interest was a cancer cell line, then a biopharmaceutical treatment could be tested for reduction in tumor size and metastasis. | ||
| - | == Structural highlights == | ||
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| - | This is a sample scene created with SAT to <scene name="/12/3456/Sample/1">color</scene> by Group, and another to make <scene name="/12/3456/Sample/2">a transparent representation</scene> of the protein. You can make your own scenes on SAT starting from scratch or loading and editing one of these sample scenes. | ||
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| - | </StructureSection> | ||
== References == | == References == | ||
<references/> | <references/> | ||
| + | https://www.rcsb.org/structure/2D1S | ||
| + | http://pdb101.rcsb.org/motm/78 | ||
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Luciferase
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