|
|
| (3 intermediate revisions not shown.) |
| Line 1: |
Line 1: |
| - | [[Image:2ehn.jpg|left|200px]] | + | #REDIRECT [[3ah4]] This PDB entry is obsolete and replaced by 3ah4 |
| - | | + | |
| - | {{Structure
| + | |
| - | |PDB= 2ehn |SIZE=350|CAPTION= <scene name='initialview01'>2ehn</scene>, resolution 1.78Å
| + | |
| - | |SITE= <scene name='pdbsite=AC1:Gal+Binding+Site+For+Residue+B+2000'>AC1</scene> and <scene name='pdbsite=AC2:Gal+Binding+Site+For+Residue+B+2001'>AC2</scene>
| + | |
| - | |LIGAND= <scene name='pdbligand=GAL:BETA-D-GALACTOSE'>GAL</scene>
| + | |
| - | |ACTIVITY=
| + | |
| - | |GENE=
| + | |
| - | |DOMAIN=
| + | |
| - | |RELATEDENTRY=[[1qxm|1QXM]], [[2ehi|2EHI]], [[2ehm|2EHM]]
| + | |
| - | |RESOURCES=<span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=2ehn FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=2ehn OCA], [http://www.ebi.ac.uk/pdbsum/2ehn PDBsum], [http://www.rcsb.org/pdb/explore.do?structureId=2ehn RCSB]</span>
| + | |
| - | }}
| + | |
| - | | + | |
| - | '''HA1 subcomponent of botulinum type C progenitor toxin complexed with galactose'''
| + | |
| - | | + | |
| - | | + | |
| - | ==Overview==
| + | |
| - | Clostridium botulinum type C 16S progenitor toxin contains a hemagglutinin (HA) subcomponent, designated HA1, which appears to play an important role in the effective internalization of the toxin in gastrointestinal epithelial cells and in creating a broad specificity for the oligosaccharide structure that corresponds to various targets. In this study, using the recombinant protein fused to glutathione S-transferase, we investigated the binding specificity of the HA1 subcomponent to sugars and estimated the binding sites of HA1 based on X-ray crystallography and soaking experiments using various sugars. N-Acetylneuraminic acid, N-acetylgalactosamine, and galactose effectively inhibited the binding that occurs between glutathione S-transferase-HA1 and mucins, whereas N-acetylglucosamine and glucose did not inhibit it. The crystal structures of HA1 complex with N-acetylneuraminic acid, N-acetylgalactosamine, and galactose were also determined. There are two sugar-binding sites, sites I and II. Site I corresponds to the electron densities noted for all sugars and is located at the C-terminal beta-trefoil domain, while site II corresponds to the electron densities noted only for galactose. An aromatic amino acid residue, Trp176, at site I has a stacking interaction with the hexose ring of the sugars. On the other hand, there is no aromatic residue at site II; thus, the interaction with galactose seems to be poor. The double mutant W176A at site I and D271F at site II has no avidity for N-acetylneuraminic acid but has avidity for galactose. In this report, the binding specificity of botulinum C16S toxin HA1 to various sugars is demonstrated based on its structural features.
| + | |
| - | | + | |
| - | ==About this Structure==
| + | |
| - | 2EHN is a [[Single protein]] structure of sequence from [http://en.wikipedia.org/wiki/Clostridium_botulinum Clostridium botulinum]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2EHN OCA].
| + | |
| - | | + | |
| - | ==Reference==
| + | |
| - | Sugar-binding sites of the HA1 subcomponent of Clostridium botulinum type C progenitor toxin., Nakamura T, Tonozuka T, Ide A, Yuzawa T, Oguma K, Nishikawa A, J Mol Biol. 2008 Feb 22;376(3):854-67. Epub 2007 Dec 23. PMID:[http://www.ncbi.nlm.nih.gov/pubmed/18178224 18178224]
| + | |
| - | [[Category: Clostridium botulinum]]
| + | |
| - | [[Category: Single protein]]
| + | |
| - | [[Category: Ide, A.]]
| + | |
| - | [[Category: Nakamura, T.]]
| + | |
| - | [[Category: Nishikawa, A.]]
| + | |
| - | [[Category: Oguma, K.]]
| + | |
| - | [[Category: Tonozuka, T.]]
| + | |
| - | [[Category: Yuzawa, T.]]
| + | |
| - | [[Category: beta trefoil]]
| + | |
| - | [[Category: toxin]]
| + | |
| - | | + | |
| - | ''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Mon Mar 31 02:49:16 2008''
| + | |
