2eig

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[[Image:2eig.jpg|left|200px]]
 
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{{Structure
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==Lotus tetragonolobus seed lectin (Isoform)==
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|PDB= 2eig |SIZE=350|CAPTION= <scene name='initialview01'>2eig</scene>, resolution 2.00&Aring;
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<StructureSection load='2eig' size='340' side='right'caption='[[2eig]], [[Resolution|resolution]] 2.00&Aring;' scene=''>
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|SITE= <scene name='pdbsite=AC1:Nag+Binding+Site+For+Residue+A+1001'>AC1</scene>, <scene name='pdbsite=AC2:Nag+Binding+Site+For+Residue+B+3001'>AC2</scene>, <scene name='pdbsite=AC3:Mn+Binding+Site+For+Residue+A+1101'>AC3</scene>, <scene name='pdbsite=AC4:Ca+Binding+Site+For+Residue+A+1102'>AC4</scene>, <scene name='pdbsite=AC5:Mn+Binding+Site+For+Residue+B+1201'>AC5</scene>, <scene name='pdbsite=AC6:Ca+Binding+Site+For+Residue+B+1202'>AC6</scene>, <scene name='pdbsite=AC7:Mn+Binding+Site+For+Residue+C+1301'>AC7</scene>, <scene name='pdbsite=AC8:Ca+Binding+Site+For+Residue+C+1302'>AC8</scene>, <scene name='pdbsite=AC9:Mn+Binding+Site+For+Residue+D+1401'>AC9</scene> and <scene name='pdbsite=BC1:Ca+Binding+Site+For+Residue+D+1402'>BC1</scene>
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== Structural highlights ==
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|LIGAND= <scene name='pdbligand=CA:CALCIUM+ION'>CA</scene>, <scene name='pdbligand=MN:MANGANESE+(II)+ION'>MN</scene>, <scene name='pdbligand=NAG:N-ACETYL-D-GLUCOSAMINE'>NAG</scene>
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<table><tr><td colspan='2'>[[2eig]] is a 4 chain structure with sequence from [https://en.wikipedia.org/wiki/Lotus_tetragonolobus Lotus tetragonolobus]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2EIG OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=2EIG FirstGlance]. <br>
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|ACTIVITY=
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</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution|Resolution]] 2&#8491;</td></tr>
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|GENE=
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<tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=CA:CALCIUM+ION'>CA</scene>, <scene name='pdbligand=MN:MANGANESE+(II)+ION'>MN</scene>, <scene name='pdbligand=NAG:N-ACETYL-D-GLUCOSAMINE'>NAG</scene></td></tr>
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|DOMAIN=
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<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=2eig FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=2eig OCA], [https://pdbe.org/2eig PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=2eig RCSB], [https://www.ebi.ac.uk/pdbsum/2eig PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=2eig ProSAT]</span></td></tr>
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|RELATEDENTRY=
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</table>
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|RESOURCES=<span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=2eig FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=2eig OCA], [http://www.ebi.ac.uk/pdbsum/2eig PDBsum], [http://www.rcsb.org/pdb/explore.do?structureId=2eig RCSB]</span>
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== Function ==
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}}
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[https://www.uniprot.org/uniprot/D0VWW1_LOTTE D0VWW1_LOTTE]
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== Evolutionary Conservation ==
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'''Lotus tetragonolobus seed lectin (Isoform)'''
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[[Image:Consurf_key_small.gif|200px|right]]
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Check<jmol>
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<jmolCheckbox>
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==Overview==
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<scriptWhenChecked>; select protein; define ~consurf_to_do selected; consurf_initial_scene = true; script "/wiki/ConSurf/ei/2eig_consurf.spt"</scriptWhenChecked>
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<scriptWhenUnchecked>script /wiki/extensions/Proteopedia/spt/initialview03.spt</scriptWhenUnchecked>
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<text>to colour the structure by Evolutionary Conservation</text>
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</jmolCheckbox>
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</jmol>, as determined by [http://consurfdb.tau.ac.il/ ConSurfDB]. You may read the [[Conservation%2C_Evolutionary|explanation]] of the method and the full data available from [http://bental.tau.ac.il/new_ConSurfDB/main_output.php?pdb_ID=2eig ConSurf].
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<div style="clear:both"></div>
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<div style="background-color:#fffaf0;">
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== Publication Abstract from PubMed ==
Lotus tetragonolobus lectin (LTA) is a fucose-specific legume lectin. Although several studies report a diverse combination of biological activities for LTA, little is known about the mechanisms involved in l-fucosyl oligosaccharide recognition. The crystal structure of LTA at 2.0A resolution reveals a different legume lectin tetramer. Its structure consists of a homotetramer composed of two back-to-back GS4-like dimers arranged in a new mode, resulting in a novel tetramer. The LTA N-linked carbohydrate at Asn4 and the unusual LTA dimer-dimer interaction are related to its particular mode of tetramerization. In addition, we used small angle X-ray scattering to investigate the quaternary structure of LTA in solution and to compare it to the crystalline structure. Although the crystal structure of LTA has revealed a conserved metal-binding site, its l-fucose-binding site presents some punctual differences. Our investigation of the new tetramer of LTA and its fucose-binding site is essential for further studies related to cross-linking between LTA and complex divalent l-fucosyl carbohydrates.
Lotus tetragonolobus lectin (LTA) is a fucose-specific legume lectin. Although several studies report a diverse combination of biological activities for LTA, little is known about the mechanisms involved in l-fucosyl oligosaccharide recognition. The crystal structure of LTA at 2.0A resolution reveals a different legume lectin tetramer. Its structure consists of a homotetramer composed of two back-to-back GS4-like dimers arranged in a new mode, resulting in a novel tetramer. The LTA N-linked carbohydrate at Asn4 and the unusual LTA dimer-dimer interaction are related to its particular mode of tetramerization. In addition, we used small angle X-ray scattering to investigate the quaternary structure of LTA in solution and to compare it to the crystalline structure. Although the crystal structure of LTA has revealed a conserved metal-binding site, its l-fucose-binding site presents some punctual differences. Our investigation of the new tetramer of LTA and its fucose-binding site is essential for further studies related to cross-linking between LTA and complex divalent l-fucosyl carbohydrates.
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==About this Structure==
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Identification of a new quaternary association for legume lectins.,Moreno FB, de Oliveira TM, Martil DE, Vicoti MM, Bezerra GA, Abrego JR, Cavada BS, Filgueira de Azevedo W Jr J Struct Biol. 2008 Feb;161(2):133-43. Epub 2007 Oct 15. PMID:18068379<ref>PMID:18068379</ref>
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2EIG is a [[Single protein]] structure of sequence from [http://en.wikipedia.org/wiki/Lotus_tetragonolobus Lotus tetragonolobus]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2EIG OCA].
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==Reference==
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From MEDLINE&reg;/PubMed&reg;, a database of the U.S. National Library of Medicine.<br>
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Identification of a new quaternary association for legume lectins., Moreno FB, de Oliveira TM, Martil DE, Vicoti MM, Bezerra GA, Abrego JR, Cavada BS, Filgueira de Azevedo W Jr, J Struct Biol. 2008 Feb;161(2):133-43. Epub 2007 Oct 15. PMID:[http://www.ncbi.nlm.nih.gov/pubmed/18068379 18068379]
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</div>
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<div class="pdbe-citations 2eig" style="background-color:#fffaf0;"></div>
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== References ==
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<references/>
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__TOC__
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</StructureSection>
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[[Category: Large Structures]]
[[Category: Lotus tetragonolobus]]
[[Category: Lotus tetragonolobus]]
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[[Category: Single protein]]
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[[Category: Abrego JRB]]
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[[Category: Abrego, J R.B.]]
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[[Category: Bezerra GA]]
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[[Category: Bezerra, G A.]]
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[[Category: Cavada BS]]
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[[Category: Cavada, B S.]]
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[[Category: Filgueira de Azevedo Jr W]]
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[[Category: Jr., W Filgueira de Azevedo.]]
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[[Category: Moreno FBMB]]
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[[Category: Moreno, F B.M B.]]
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[[Category: Vicoti MM]]
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[[Category: Oliveira, T M.de.]]
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[[Category: De Oliveira TM]]
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[[Category: Vicoti, M M.]]
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[[Category: l-fucosyl]]
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[[Category: lotus tetragonolobus]]
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[[Category: n-acetyl-d-glucosamine]]
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[[Category: sugar binding protein]]
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''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Mon Mar 31 02:49:35 2008''
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Current revision

Lotus tetragonolobus seed lectin (Isoform)

PDB ID 2eig

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