4qx3

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==Cry3A Toxin structure obtained by injecting Bacillus thuringiensis cells in an XFEL beam, collecting data by serial femtosecond crystallographic methods and processing data with the CrystFEL software suite==
==Cry3A Toxin structure obtained by injecting Bacillus thuringiensis cells in an XFEL beam, collecting data by serial femtosecond crystallographic methods and processing data with the CrystFEL software suite==
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<StructureSection load='4qx3' size='340' side='right' caption='[[4qx3]], [[Resolution|resolution]] 2.90&Aring;' scene=''>
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<StructureSection load='4qx3' size='340' side='right'caption='[[4qx3]], [[Resolution|resolution]] 2.90&Aring;' scene=''>
== Structural highlights ==
== Structural highlights ==
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<table><tr><td colspan='2'>[[4qx3]] is a 1 chain structure with sequence from [http://en.wikipedia.org/wiki/Bactt Bactt]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=4QX3 OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=4QX3 FirstGlance]. <br>
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<table><tr><td colspan='2'>[[4qx3]] is a 1 chain structure with sequence from [https://en.wikipedia.org/wiki/Bacillus_thuringiensis_serovar_tenebrionis Bacillus thuringiensis serovar tenebrionis]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=4QX3 OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=4QX3 FirstGlance]. <br>
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</td></tr><tr id='related'><td class="sblockLbl"><b>[[Related_structure|Related:]]</b></td><td class="sblockDat">[[4qx0|4qx0]], [[4qx1|4qx1]], [[4qx2|4qx2]]</td></tr>
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</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution|Resolution]] 2.9&#8491;</td></tr>
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<tr id='gene'><td class="sblockLbl"><b>[[Gene|Gene:]]</b></td><td class="sblockDat">bt13, cry3A, cry3Aa, cryC, cryIIIa, cryIIIA(a) ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=1444 BACTT])</td></tr>
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<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=4qx3 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=4qx3 OCA], [https://pdbe.org/4qx3 PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=4qx3 RCSB], [https://www.ebi.ac.uk/pdbsum/4qx3 PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=4qx3 ProSAT]</span></td></tr>
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<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=4qx3 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=4qx3 OCA], [http://pdbe.org/4qx3 PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=4qx3 RCSB], [http://www.ebi.ac.uk/pdbsum/4qx3 PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=4qx3 ProSAT]</span></td></tr>
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</table>
</table>
== Function ==
== Function ==
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[[http://www.uniprot.org/uniprot/CR3AA_BACTT CR3AA_BACTT]] Promotes colloidosmotic lysis by binding to the midgut epithelial cells of Coleoptera.
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[https://www.uniprot.org/uniprot/CR3AA_BACTT CR3AA_BACTT] Promotes colloidosmotic lysis by binding to the midgut epithelial cells of Coleoptera.
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<div style="background-color:#fffaf0;">
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== Publication Abstract from PubMed ==
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It has long been known that toxins produced by Bacillus thuringiensis (Bt) are stored in the bacterial cells in crystalline form. Here we describe the structure determination of the Cry3A toxin found naturally crystallized within Bt cells. When whole Bt cells were streamed into an X-ray free-electron laser beam we found that scattering from other cell components did not obscure diffraction from the crystals. The resolution limits of the best diffraction images collected from cells were the same as from isolated crystals. The integrity of the cells at the moment of diffraction is unclear; however, given the short time ( approximately 5 micros) between exiting the injector to intersecting with the X-ray beam, our result is a 2.9-A-resolution structure of a crystalline protein as it exists in a living cell. The study suggests that authentic in vivo diffraction studies can produce atomic-level structural information.
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Protein crystal structure obtained at 2.9 A resolution from injecting bacterial cells into an X-ray free-electron laser beam.,Sawaya MR, Cascio D, Gingery M, Rodriguez J, Goldschmidt L, Colletier JP, Messerschmidt MM, Boutet S, Koglin JE, Williams GJ, Brewster AS, Nass K, Hattne J, Botha S, Doak RB, Shoeman RL, DePonte DP, Park HW, Federici BA, Sauter NK, Schlichting I, Eisenberg DS Proc Natl Acad Sci U S A. 2014 Aug 18. pii: 201413456. PMID:25136092<ref>PMID:25136092</ref>
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==See Also==
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*[[Delta-endotoxin|Delta-endotoxin]]
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From MEDLINE&reg;/PubMed&reg;, a database of the U.S. National Library of Medicine.<br>
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*[[Pesticidal crystal protein|Pesticidal crystal protein]]
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</div>
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<div class="pdbe-citations 4qx3" style="background-color:#fffaf0;"></div>
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== References ==
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<references/>
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__TOC__
__TOC__
</StructureSection>
</StructureSection>
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[[Category: Bactt]]
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[[Category: Bacillus thuringiensis serovar tenebrionis]]
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[[Category: Botha, S]]
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[[Category: Large Structures]]
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[[Category: Boutet, S]]
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[[Category: Botha S]]
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[[Category: Brewster, A S]]
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[[Category: Boutet S]]
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[[Category: Cascio, D]]
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[[Category: Brewster AS]]
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[[Category: Colletier, J P]]
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[[Category: Cascio D]]
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[[Category: DePonte, D P]]
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[[Category: Colletier J-P]]
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[[Category: Doak, R B]]
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[[Category: DePonte DP]]
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[[Category: Eisenberg, D]]
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[[Category: Doak RB]]
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[[Category: Federici, B A]]
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[[Category: Eisenberg D]]
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[[Category: Gingery, M]]
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[[Category: Federici BA]]
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[[Category: Goldschmidt, L]]
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[[Category: Gingery M]]
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[[Category: Hattne, J]]
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[[Category: Goldschmidt L]]
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[[Category: Koglin, J E]]
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[[Category: Hattne J]]
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[[Category: Messerschmidt, M]]
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[[Category: Koglin JE]]
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[[Category: Nass, K]]
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[[Category: Messerschmidt M]]
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[[Category: Park, H W]]
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[[Category: Nass K]]
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[[Category: Rodriguez, J]]
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[[Category: Park H-W]]
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[[Category: Sauter, N K]]
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[[Category: Rodriguez J]]
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[[Category: Sawaya, M R]]
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[[Category: Sauter NK]]
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[[Category: Schlichting, I]]
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[[Category: Sawaya MR]]
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[[Category: Shoeman, R L]]
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[[Category: Schlichting I]]
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[[Category: Williams, G J]]
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[[Category: Shoeman RL]]
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[[Category: In vivo crystal]]
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[[Category: Williams GJ]]
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[[Category: Insecticidal toxin]]
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[[Category: Lcl]]
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[[Category: Microcrystal]]
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[[Category: Serial femtosecond crystallography]]
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[[Category: Sfx]]
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[[Category: Toxin]]
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[[Category: X-ray free-electron laser]]
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Current revision

Cry3A Toxin structure obtained by injecting Bacillus thuringiensis cells in an XFEL beam, collecting data by serial femtosecond crystallographic methods and processing data with the CrystFEL software suite

PDB ID 4qx3

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