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| | ==Solution structure of the C-terminal RRM of human La (La225-334)== | | ==Solution structure of the C-terminal RRM of human La (La225-334)== |
| - | <StructureSection load='1owx' size='340' side='right' caption='[[1owx]], [[NMR_Ensembles_of_Models | 20 NMR models]]' scene=''> | + | <StructureSection load='1owx' size='340' side='right'caption='[[1owx]]' scene=''> |
| | == Structural highlights == | | == Structural highlights == |
| - | <table><tr><td colspan='2'>[[1owx]] is a 1 chain structure with sequence from [http://en.wikipedia.org/wiki/Human Human]. Full experimental information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1OWX OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=1OWX FirstGlance]. <br> | + | <table><tr><td colspan='2'>[[1owx]] is a 1 chain structure with sequence from [https://en.wikipedia.org/wiki/Homo_sapiens Homo sapiens]. Full experimental information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1OWX OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=1OWX FirstGlance]. <br> |
| - | </td></tr><tr id='gene'><td class="sblockLbl"><b>[[Gene|Gene:]]</b></td><td class="sblockDat">SSB ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=9606 HUMAN])</td></tr> | + | </td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">Solution NMR</td></tr> |
| - | <tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=1owx FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1owx OCA], [http://pdbe.org/1owx PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=1owx RCSB], [http://www.ebi.ac.uk/pdbsum/1owx PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=1owx ProSAT]</span></td></tr> | + | <tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=1owx FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1owx OCA], [https://pdbe.org/1owx PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=1owx RCSB], [https://www.ebi.ac.uk/pdbsum/1owx PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=1owx ProSAT]</span></td></tr> |
| | </table> | | </table> |
| | == Function == | | == Function == |
| - | [[http://www.uniprot.org/uniprot/LA_HUMAN LA_HUMAN]] Binds to the 3' poly(U) terminii of nascent RNA polymerase III transcripts, protecting them from exonuclease digestion and facilitating their folding and maturation.<ref>PMID:3192525</ref> <ref>PMID:2470590</ref> | + | [https://www.uniprot.org/uniprot/LA_HUMAN LA_HUMAN] Binds to the 3' poly(U) terminii of nascent RNA polymerase III transcripts, protecting them from exonuclease digestion and facilitating their folding and maturation.<ref>PMID:3192525</ref> <ref>PMID:2470590</ref> |
| | == Evolutionary Conservation == | | == Evolutionary Conservation == |
| | [[Image:Consurf_key_small.gif|200px|right]] | | [[Image:Consurf_key_small.gif|200px|right]] |
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| | __TOC__ | | __TOC__ |
| | </StructureSection> | | </StructureSection> |
| - | [[Category: Human]] | + | [[Category: Homo sapiens]] |
| - | [[Category: Babon, J]] | + | [[Category: Large Structures]] |
| - | [[Category: Cary, P D]] | + | [[Category: Babon J]] |
| - | [[Category: Conte, M R]] | + | [[Category: Cary PD]] |
| - | [[Category: Curry, S]] | + | [[Category: Conte MR]] |
| - | [[Category: Jacks, A]] | + | [[Category: Curry S]] |
| - | [[Category: Kelly, G]] | + | [[Category: Jacks A]] |
| - | [[Category: Manolaridis, I]] | + | [[Category: Kelly G]] |
| - | [[Category: Rrm]]
| + | [[Category: Manolaridis I]] |
| - | [[Category: Transcription]]
| + | |
| Structural highlights
Function
LA_HUMAN Binds to the 3' poly(U) terminii of nascent RNA polymerase III transcripts, protecting them from exonuclease digestion and facilitating their folding and maturation.[1] [2]
Evolutionary Conservation
Check, as determined by ConSurfDB. You may read the explanation of the method and the full data available from ConSurf.
Publication Abstract from PubMed
The La protein is an important component of ribonucleoprotein complexes that acts mainly as an RNA chaperone to facilitate correct processing and maturation of RNA polymerase III transcripts, but can also stimulate translation initiation. We report here the structure of the C-terminal domain of human La, which comprises an atypical RNA recognition motif (La225-334) and a long unstructured C-terminal tail. The central beta sheet of La225-334 reveals novel features: the putative RNA binding surface is formed by a five-stranded beta sheet and, strikingly, is largely obscured by a long C-terminal alpha helix that encompasses a recently identified nuclear retention element. Contrary to previous observations, we find that the La protein does not contain a dimerization domain.
Structure of the C-terminal domain of human La protein reveals a novel RNA recognition motif coupled to a helical nuclear retention element.,Jacks A, Babon J, Kelly G, Manolaridis I, Cary PD, Curry S, Conte MR Structure. 2003 Jul;11(7):833-43. PMID:12842046[3]
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.
References
- ↑ Chambers JC, Kenan D, Martin BJ, Keene JD. Genomic structure and amino acid sequence domains of the human La autoantigen. J Biol Chem. 1988 Dec 5;263(34):18043-51. PMID:3192525
- ↑ Gottlieb E, Steitz JA. Function of the mammalian La protein: evidence for its action in transcription termination by RNA polymerase III. EMBO J. 1989 Mar;8(3):851-61. PMID:2470590
- ↑ Jacks A, Babon J, Kelly G, Manolaridis I, Cary PD, Curry S, Conte MR. Structure of the C-terminal domain of human La protein reveals a novel RNA recognition motif coupled to a helical nuclear retention element. Structure. 2003 Jul;11(7):833-43. PMID:12842046
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