2emd

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[[Image:2emd.jpg|left|200px]]
 
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{{Structure
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==GREEN FLUORESCENT PROTEIN FROM AEQUOREA VICTORIA, MUTANT==
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|PDB= 2emd |SIZE=350|CAPTION= <scene name='initialview01'>2emd</scene>, resolution 2.0&Aring;
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<StructureSection load='2emd' size='340' side='right'caption='[[2emd]], [[Resolution|resolution]] 2.00&Aring;' scene=''>
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|SITE= <scene name='pdbsite=CSH:The+Chromophore+(Csh)+Is+Formed+From+SER+65+-+TYR+66+-+G+...'>CSH</scene>
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== Structural highlights ==
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|LIGAND= <scene name='pdbligand=CSH:[2-(2-HYDROXY-1-METHYL-ETHYL)-4-(1H-IMIDAZOL-4-YLMETHYL)-5-OXO-IMIDAZOLIDIN-1-YL]-ACETIC+ACID'>CSH</scene>
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<table><tr><td colspan='2'>[[2emd]] is a 1 chain structure with sequence from [https://en.wikipedia.org/wiki/Aequorea_victoria Aequorea victoria]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2EMD OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=2EMD FirstGlance]. <br>
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|ACTIVITY=
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</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution|Resolution]] 2&#8491;</td></tr>
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|GENE= GFP ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=6100 Aequorea victoria])
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<tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=CSH:[2-(2-HYDROXY-1-METHYL-ETHYL)-4-(1H-IMIDAZOL-4-YLMETHYL)-5-OXO-IMIDAZOLIDIN-1-YL]-ACETIC+ACID'>CSH</scene></td></tr>
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|DOMAIN=
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<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=2emd FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=2emd OCA], [https://pdbe.org/2emd PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=2emd RCSB], [https://www.ebi.ac.uk/pdbsum/2emd PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=2emd ProSAT]</span></td></tr>
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|RELATEDENTRY=
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</table>
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|RESOURCES=<span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=2emd FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=2emd OCA], [http://www.ebi.ac.uk/pdbsum/2emd PDBsum], [http://www.rcsb.org/pdb/explore.do?structureId=2emd RCSB]</span>
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== Function ==
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}}
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[https://www.uniprot.org/uniprot/GFP_AEQVI GFP_AEQVI] Energy-transfer acceptor. Its role is to transduce the blue chemiluminescence of the protein aequorin into green fluorescent light by energy transfer. Fluoresces in vivo upon receiving energy from the Ca(2+)-activated photoprotein aequorin.
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== Evolutionary Conservation ==
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[[Image:Consurf_key_small.gif|200px|right]]
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Check<jmol>
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<jmolCheckbox>
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<scriptWhenChecked>; select protein; define ~consurf_to_do selected; consurf_initial_scene = true; script "/wiki/ConSurf/em/2emd_consurf.spt"</scriptWhenChecked>
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<scriptWhenUnchecked>script /wiki/extensions/Proteopedia/spt/initialview03.spt</scriptWhenUnchecked>
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<text>to colour the structure by Evolutionary Conservation</text>
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</jmolCheckbox>
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</jmol>, as determined by [http://consurfdb.tau.ac.il/ ConSurfDB]. You may read the [[Conservation%2C_Evolutionary|explanation]] of the method and the full data available from [http://bental.tau.ac.il/new_ConSurfDB/main_output.php?pdb_ID=2emd ConSurf].
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<div style="clear:both"></div>
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'''GREEN FLUORESCENT PROTEIN FROM AEQUOREA VICTORIA, MUTANT'''
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==See Also==
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*[[Green Fluorescent Protein 3D structures|Green Fluorescent Protein 3D structures]]
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__TOC__
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==About this Structure==
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</StructureSection>
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2EMD is a [[Single protein]] structure of sequence from [http://en.wikipedia.org/wiki/Aequorea_victoria Aequorea victoria]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2EMD OCA].
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==Reference==
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The structural basis for spectral variations in green fluorescent protein., Palm GJ, Zdanov A, Gaitanaris GA, Stauber R, Pavlakis GN, Wlodawer A, Nat Struct Biol. 1997 May;4(5):361-5. PMID:[http://www.ncbi.nlm.nih.gov/pubmed/9145105 9145105]
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[[Category: Aequorea victoria]]
[[Category: Aequorea victoria]]
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[[Category: Single protein]]
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[[Category: Large Structures]]
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[[Category: Palm, G.]]
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[[Category: Palm G]]
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[[Category: Wlodawer, A.]]
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[[Category: Wlodawer A]]
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[[Category: Zdanov, A.]]
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[[Category: Zdanov A]]
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[[Category: beta-barrel]]
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[[Category: bioluminescence]]
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[[Category: fluorescent protein]]
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[[Category: luminescence]]
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''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Mon Mar 31 02:51:08 2008''
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Current revision

GREEN FLUORESCENT PROTEIN FROM AEQUOREA VICTORIA, MUTANT

PDB ID 2emd

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