1pdp
From Proteopedia
(Difference between revisions)
| (3 intermediate revisions not shown.) | |||
| Line 1: | Line 1: | ||
==Fitting of gp9 structure into the bacteriophage T4 baseplate cryoEM reconstruction== | ==Fitting of gp9 structure into the bacteriophage T4 baseplate cryoEM reconstruction== | ||
| - | < | + | <SX load='1pdp' size='340' side='right' viewer='molstar' caption='[[1pdp]], [[Resolution|resolution]] 12.00Å' scene=''> |
== Structural highlights == | == Structural highlights == | ||
| - | <table><tr><td colspan='2'>[[1pdp]] is a 18 chain structure with sequence from [ | + | <table><tr><td colspan='2'>[[1pdp]] is a 18 chain structure with sequence from [https://en.wikipedia.org/wiki/Escherichia_virus_T4 Escherichia virus T4]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1PDP OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=1PDP FirstGlance]. <br> |
| - | </td></tr><tr id=' | + | </td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">Electron Microscopy, [[Resolution|Resolution]] 12Å</td></tr> |
| - | <tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[ | + | <tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=1pdp FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1pdp OCA], [https://pdbe.org/1pdp PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=1pdp RCSB], [https://www.ebi.ac.uk/pdbsum/1pdp PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=1pdp ProSAT]</span></td></tr> |
</table> | </table> | ||
== Function == | == Function == | ||
| - | [ | + | [https://www.uniprot.org/uniprot/BP09_BPT4 BP09_BPT4] Baseplate protein that connects the long tail fibers to the baseplate and probably triggers the tail contraction after virus attachment to a host cell (PubMed:10545330). Involved in the tail assembly (PubMed:21129200).<ref>PMID:10545330</ref> <ref>PMID:21129200</ref> |
== Evolutionary Conservation == | == Evolutionary Conservation == | ||
[[Image:Consurf_key_small.gif|200px|right]] | [[Image:Consurf_key_small.gif|200px|right]] | ||
| Line 19: | Line 19: | ||
</jmol>, as determined by [http://consurfdb.tau.ac.il/ ConSurfDB]. You may read the [[Conservation%2C_Evolutionary|explanation]] of the method and the full data available from [http://bental.tau.ac.il/new_ConSurfDB/main_output.php?pdb_ID=1pdp ConSurf]. | </jmol>, as determined by [http://consurfdb.tau.ac.il/ ConSurfDB]. You may read the [[Conservation%2C_Evolutionary|explanation]] of the method and the full data available from [http://bental.tau.ac.il/new_ConSurfDB/main_output.php?pdb_ID=1pdp ConSurf]. | ||
<div style="clear:both"></div> | <div style="clear:both"></div> | ||
| - | <div style="background-color:#fffaf0;"> | ||
| - | == Publication Abstract from PubMed == | ||
| - | The baseplate of bacteriophage T4 is a multiprotein molecular machine that controls host cell recognition, attachment, tail sheath contraction and viral DNA ejection. We report here the three-dimensional structure of the baseplate-tail tube complex determined to a resolution of 12 A by cryoelectron microscopy. The baseplate has a six-fold symmetric, dome-like structure approximately 520 A in diameter and approximately 270 A long, assembled around a central hub. A 940 A-long and 96 A-diameter tail tube, coaxial with the hub, is connected to the top of the baseplate. At the center of the dome is a needle-like structure that was previously identified as a cell puncturing device. We have identified the locations of six proteins with known atomic structures, and established the position and shape of several other baseplate proteins. The baseplate structure suggests a mechanism of baseplate triggering and structural transition during the initial stages of T4 infection. | ||
| - | |||
| - | Three-dimensional structure of bacteriophage T4 baseplate.,Kostyuchenko VA, Leiman PG, Chipman PR, Kanamaru S, van Raaij MJ, Arisaka F, Mesyanzhinov VV, Rossmann MG Nat Struct Biol. 2003 Sep;10(9):688-93. Epub 2003 Aug 17. PMID:12923574<ref>PMID:12923574</ref> | ||
| - | |||
| - | From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br> | ||
| - | </div> | ||
| - | <div class="pdbe-citations 1pdp" style="background-color:#fffaf0;"></div> | ||
== References == | == References == | ||
<references/> | <references/> | ||
__TOC__ | __TOC__ | ||
| - | </ | + | </SX> |
| - | [[Category: | + | [[Category: Escherichia virus T4]] |
| - | [[Category: Arisaka | + | [[Category: Large Structures]] |
| - | [[Category: Chipman | + | [[Category: Arisaka F]] |
| - | [[Category: Kanamaru | + | [[Category: Chipman PR]] |
| - | [[Category: Kostyuchenko | + | [[Category: Kanamaru S]] |
| - | [[Category: Leiman | + | [[Category: Kostyuchenko VA]] |
| - | [[Category: Mesyanzhinov | + | [[Category: Leiman PG]] |
| - | + | [[Category: Mesyanzhinov VV]] | |
| - | [[Category: Rossmann | + | [[Category: Rossmann MG]] |
| - | [[Category: | + | [[Category: Van Raaij MJ]] |
Current revision
Fitting of gp9 structure into the bacteriophage T4 baseplate cryoEM reconstruction
| |||||||||
