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| | ==Solution structure of the N-terminal PH/PTB domain of the TFIIH P62 subunit== | | ==Solution structure of the N-terminal PH/PTB domain of the TFIIH P62 subunit== |
| - | <StructureSection load='1pfj' size='340' side='right' caption='[[1pfj]], [[NMR_Ensembles_of_Models | 19 NMR models]]' scene=''> | + | <StructureSection load='1pfj' size='340' side='right'caption='[[1pfj]]' scene=''> |
| | == Structural highlights == | | == Structural highlights == |
| - | <table><tr><td colspan='2'>[[1pfj]] is a 1 chain structure with sequence from [http://en.wikipedia.org/wiki/Human Human]. Full experimental information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1PFJ OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=1PFJ FirstGlance]. <br> | + | <table><tr><td colspan='2'>[[1pfj]] is a 1 chain structure with sequence from [https://en.wikipedia.org/wiki/Homo_sapiens Homo sapiens]. Full experimental information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1PFJ OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=1PFJ FirstGlance]. <br> |
| - | </td></tr><tr id='gene'><td class="sblockLbl"><b>[[Gene|Gene:]]</b></td><td class="sblockDat">GTF2H1 OR BTF2 ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=9606 HUMAN])</td></tr> | + | </td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">Solution NMR</td></tr> |
| - | <tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=1pfj FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1pfj OCA], [http://pdbe.org/1pfj PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=1pfj RCSB], [http://www.ebi.ac.uk/pdbsum/1pfj PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=1pfj ProSAT]</span></td></tr> | + | <tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=1pfj FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1pfj OCA], [https://pdbe.org/1pfj PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=1pfj RCSB], [https://www.ebi.ac.uk/pdbsum/1pfj PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=1pfj ProSAT]</span></td></tr> |
| | </table> | | </table> |
| | == Function == | | == Function == |
| - | [[http://www.uniprot.org/uniprot/TF2H1_HUMAN TF2H1_HUMAN]] Component of the core-TFIIH basal transcription factor involved in nucleotide excision repair (NER) of DNA and, when complexed to CAK, in RNA transcription by RNA polymerase II. | + | [https://www.uniprot.org/uniprot/TF2H1_HUMAN TF2H1_HUMAN] Component of the core-TFIIH basal transcription factor involved in nucleotide excision repair (NER) of DNA and, when complexed to CAK, in RNA transcription by RNA polymerase II. |
| | == Evolutionary Conservation == | | == Evolutionary Conservation == |
| | [[Image:Consurf_key_small.gif|200px|right]] | | [[Image:Consurf_key_small.gif|200px|right]] |
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| | __TOC__ | | __TOC__ |
| | </StructureSection> | | </StructureSection> |
| - | [[Category: Human]] | + | [[Category: Homo sapiens]] |
| - | [[Category: Frindel, F]] | + | [[Category: Large Structures]] |
| - | [[Category: Gervais, V]] | + | [[Category: Frindel F]] |
| - | [[Category: Jawhari, A]] | + | [[Category: Gervais V]] |
| - | [[Category: Kieffer, B]] | + | [[Category: Jawhari A]] |
| - | [[Category: Lamour, V]] | + | [[Category: Kieffer B]] |
| - | [[Category: Poterszman, A]] | + | [[Category: Lamour V]] |
| - | [[Category: SPINE, Structural Proteomics in Europe]]
| + | [[Category: Poterszman A]] |
| - | [[Category: Thierry, J C]] | + | [[Category: Thierry JC]] |
| - | [[Category: Wasielewski, E]] | + | [[Category: Wasielewski E]] |
| - | [[Category: Ph/ptb domain]]
| + | |
| - | [[Category: Spine]]
| + | |
| - | [[Category: Structural genomic]]
| + | |
| - | [[Category: Structural proteomics in europe]]
| + | |
| - | [[Category: Transcription]]
| + | |
| Structural highlights
Function
TF2H1_HUMAN Component of the core-TFIIH basal transcription factor involved in nucleotide excision repair (NER) of DNA and, when complexed to CAK, in RNA transcription by RNA polymerase II.
Evolutionary Conservation
Check, as determined by ConSurfDB. You may read the explanation of the method and the full data available from ConSurf.
Publication Abstract from PubMed
The human general transcription factor TFIIH is involved in both transcription and DNA repair. We have identified a structural domain in the core subunit of TFIIH, p62, which is absolutely required for DNA repair activity through the nucleotide excision repair pathway. Using coimmunoprecipitation experiments, we showed that this activity involves the interaction between the N-terminal domain of p62 and the 3' endonuclease XPG, a major component of the nucleotide excision repair machinery. Furthermore, we reconstituted a functional TFIIH particle with a mutant of p62 lacking the N-terminal domain, showing that this domain is not required for assembly of the TFIIH complex and basal transcription. We solved its three-dimensional structure and found an unpredicted pleckstrin homology and phosphotyrosine binding (PH/PTB) domain, uncovering a new class of activity for this fold.
TFIIH contains a PH domain involved in DNA nucleotide excision repair.,Gervais V, Lamour V, Jawhari A, Frindel F, Wasielewski E, Dubaele S, Egly JM, Thierry JC, Kieffer B, Poterszman A Nat Struct Mol Biol. 2004 Jul;11(7):616-22. Epub 2004 Jun 13. PMID:15195146[1]
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.
References
- ↑ Gervais V, Lamour V, Jawhari A, Frindel F, Wasielewski E, Dubaele S, Egly JM, Thierry JC, Kieffer B, Poterszman A. TFIIH contains a PH domain involved in DNA nucleotide excision repair. Nat Struct Mol Biol. 2004 Jul;11(7):616-22. Epub 2004 Jun 13. PMID:15195146 doi:http://dx.doi.org/10.1038/nsmb782
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