1ovg

<StructureSection load='1ovg' size='340' side='right' caption='[[1ovg]], [[Resolution|resolution]] 2.20&Aring;' scene=''>

<table><tr><td colspan='2'>[[1ovg]] is a 3 chain structure with sequence from [http://en.wikipedia.org/wiki/"bacillus_coli"_migula_1895 "bacillus coli" migula 1895]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1OVG OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=1OVG FirstGlance]. <br>

</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=MDR:9-(2-DEOXY-BETA-D-RIBOFURANOSYL)-6-METHYLPURINE'>MDR</scene>, <scene name='pdbligand=PO4:PHOSPHATE+ION'>PO4</scene></td></tr>

<tr id='related'><td class="sblockLbl"><b>[[Related_structure|Related:]]</b></td><td class="sblockDat">[[1ecp|1ecp]], [[1a9o|1a9o]], [[1otx|1otx]], [[1oty|1oty]], [[1ou4|1ou4]], [[1oum|1oum]], [[1ov6|1ov6]]</td></tr>

<tr id='gene'><td class="sblockLbl"><b>[[Gene|Gene:]]</b></td><td class="sblockDat">DEOD OR PUP OR B4384 OR Z5986 OR ECS5343 ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=562 "Bacillus coli" Migula 1895])</td></tr>

<tr id='activity'><td class="sblockLbl"><b>Activity:</b></td><td class="sblockDat"><span class='plainlinks'>[http://en.wikipedia.org/wiki/Purine-nucleoside_phosphorylase Purine-nucleoside phosphorylase], with EC number [http://www.brenda-enzymes.info/php/result_flat.php4?ecno=2.4.2.1 2.4.2.1] </span></td></tr>

<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=1ovg FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1ovg OCA], [http://pdbe.org/1ovg PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=1ovg RCSB], [http://www.ebi.ac.uk/pdbsum/1ovg PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=1ovg ProSAT]</span></td></tr>

[[http://www.uniprot.org/uniprot/DEOD_ECOLI DEOD_ECOLI]] Cleavage of guanosine or inosine to respective bases and sugar-1-phosphate molecules.[HAMAP-Rule:MF_01627]

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== Publication Abstract from PubMed ==

Activation of prodrugs by Escherichia coli purine nucleoside phosphorylase (PNP) provides a method for selectively killing tumor cells expressing a transfected PNP gene. This gene therapy approach requires matching a prodrug and a known enzymatic activity present only in tumor cells. The specificity of the method relies on avoiding prodrug cleavage by enzymes already present in the host cells or the intestinal flora. Using crystallographic and computer modeling methods as guides, we have redesigned E. coli PNP to cleave new prodrug substrates more efficiently than does the wild-type enzyme. In particular, the M64V PNP mutant cleaves 9-(6-deoxy-alpha-L-talofuranosyl)-6-methylpurine with a kcat/Km over 100 times greater than for native E. coli PNP. In a xenograft tumor experiment, this compound caused regression of tumors expressing the M64V PNP gene.

 

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== References ==

[[Category: Bacillus coli migula 1895]]

[[Category: Purine-nucleoside phosphorylase]]

[[Category: Allan, P W]]

[[Category: Anand, R]]

[[Category: Bennett, E M]]

[[Category: Ealick, S E]]

[[Category: Hassan, A E]]

[[Category: McPherson, D T]]

[[Category: Parker, P W]]

[[Category: Secrist, J A]]

[[Category: Sorscher, E J]]

[[Category: Transferase]]