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| | ==Human antibody fragment (Fab) to meningococcal Factor H binding protein== | | ==Human antibody fragment (Fab) to meningococcal Factor H binding protein== |
| - | <StructureSection load='5ur8' size='340' side='right' caption='[[5ur8]], [[Resolution|resolution]] 1.76Å' scene=''> | + | <StructureSection load='5ur8' size='340' side='right'caption='[[5ur8]], [[Resolution|resolution]] 1.76Å' scene=''> |
| | == Structural highlights == | | == Structural highlights == |
| - | <table><tr><td colspan='2'>[[5ur8]] is a 2 chain structure with sequence from [http://en.wikipedia.org/wiki/Human Human]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=5UR8 OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=5UR8 FirstGlance]. <br> | + | <table><tr><td colspan='2'>[[5ur8]] is a 2 chain structure with sequence from [https://en.wikipedia.org/wiki/Homo_sapiens Homo sapiens]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=5UR8 OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=5UR8 FirstGlance]. <br> |
| - | </td></tr><tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=5ur8 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=5ur8 OCA], [http://pdbe.org/5ur8 PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=5ur8 RCSB], [http://www.ebi.ac.uk/pdbsum/5ur8 PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=5ur8 ProSAT]</span></td></tr> | + | </td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution|Resolution]] 1.7550023Å</td></tr> |
| | + | <tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=5ur8 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=5ur8 OCA], [https://pdbe.org/5ur8 PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=5ur8 RCSB], [https://www.ebi.ac.uk/pdbsum/5ur8 PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=5ur8 ProSAT]</span></td></tr> |
| | </table> | | </table> |
| | <div style="background-color:#fffaf0;"> | | <div style="background-color:#fffaf0;"> |
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| | __TOC__ | | __TOC__ |
| | </StructureSection> | | </StructureSection> |
| - | [[Category: Human]] | + | [[Category: Homo sapiens]] |
| - | [[Category: Beernink, P T]] | + | [[Category: Large Structures]] |
| - | [[Category: Fab]] | + | [[Category: Beernink PT]] |
| - | [[Category: Factor h binding protein]]
| + | |
| - | [[Category: Fragment antigen binding]]
| + | |
| - | [[Category: Immune system]]
| + | |
| - | [[Category: Immunoglobulin gamma]]
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| Structural highlights
Publication Abstract from PubMed
Data obtained recently in the United Kingdom following a nationwide infant immunization program against serogroup B Neisseria meningitidis (MenB) reported >80% 4CMenB vaccine-mediated protection. Factor H-binding protein (fHbp) is a meningococcal virulence factor and a component of two new MenB vaccines. Here, we investigated the structural bases underlying the fHbp-dependent protective antibody response in humans, which might inform future antigen design efforts. We present the co-crystal structure of a human antibody Fab targeting fHbp. The vaccine-elicited Fab 1A12 is cross-reactive and targets an epitope highly conserved across the repertoire of three naturally occurring fHbp variants. The free Fab structure highlights conformational rearrangements occurring upon antigen binding. Importantly, 1A12 is bactericidal against MenB strains expressing fHbp from all three variants. Our results reveal important immunological features potentially contributing to the broad protection conferred by fHbp vaccination. Our studies fuel the rationale of presenting conserved protein epitopes when developing broadly protective vaccines.
Crystal structure reveals vaccine elicited bactericidal human antibody targeting a conserved epitope on meningococcal fHbp.,Lopez-Sagaseta J, Beernink PT, Bianchi F, Santini L, Frigimelica E, Lucas AH, Pizza M, Bottomley MJ Nat Commun. 2018 Feb 6;9(1):528. doi: 10.1038/s41467-018-02827-7. PMID:29410413[1]
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.
References
- ↑ Lopez-Sagaseta J, Beernink PT, Bianchi F, Santini L, Frigimelica E, Lucas AH, Pizza M, Bottomley MJ. Crystal structure reveals vaccine elicited bactericidal human antibody targeting a conserved epitope on meningococcal fHbp. Nat Commun. 2018 Feb 6;9(1):528. doi: 10.1038/s41467-018-02827-7. PMID:29410413 doi:http://dx.doi.org/10.1038/s41467-018-02827-7
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