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| | ==Crystal structure of the chromodomain of RBBP1== | | ==Crystal structure of the chromodomain of RBBP1== |
| - | <StructureSection load='6bph' size='340' side='right' caption='[[6bph]], [[Resolution|resolution]] 1.85Å' scene=''> | + | <StructureSection load='6bph' size='340' side='right'caption='[[6bph]], [[Resolution|resolution]] 1.85Å' scene=''> |
| | == Structural highlights == | | == Structural highlights == |
| - | <table><tr><td colspan='2'>[[6bph]] is a 1 chain structure with sequence from [http://en.wikipedia.org/wiki/Human Human]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=6BPH OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=6BPH FirstGlance]. <br> | + | <table><tr><td colspan='2'>[[6bph]] is a 1 chain structure with sequence from [https://en.wikipedia.org/wiki/Homo_sapiens Homo sapiens]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=6BPH OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=6BPH FirstGlance]. <br> |
| - | </td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=UNX:UNKNOWN+ATOM+OR+ION'>UNX</scene></td></tr> | + | </td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution|Resolution]] 1.85Å</td></tr> |
| - | <tr id='gene'><td class="sblockLbl"><b>[[Gene|Gene:]]</b></td><td class="sblockDat">ARID4A, RBBP1, RBP1 ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=9606 HUMAN])</td></tr>
| + | <tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=6bph FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=6bph OCA], [https://pdbe.org/6bph PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=6bph RCSB], [https://www.ebi.ac.uk/pdbsum/6bph PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=6bph ProSAT]</span></td></tr> |
| - | <tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=6bph FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=6bph OCA], [http://pdbe.org/6bph PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=6bph RCSB], [http://www.ebi.ac.uk/pdbsum/6bph PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=6bph ProSAT]</span></td></tr> | + | |
| | </table> | | </table> |
| | == Function == | | == Function == |
| - | [[http://www.uniprot.org/uniprot/ARI4A_HUMAN ARI4A_HUMAN]] Interacts with the viral protein-binding domain of the retinoblastoma protein. | + | [https://www.uniprot.org/uniprot/ARI4A_HUMAN ARI4A_HUMAN] Interacts with the viral protein-binding domain of the retinoblastoma protein. |
| | <div style="background-color:#fffaf0;"> | | <div style="background-color:#fffaf0;"> |
| | == Publication Abstract from PubMed == | | == Publication Abstract from PubMed == |
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| | __TOC__ | | __TOC__ |
| | </StructureSection> | | </StructureSection> |
| - | [[Category: Human]] | + | [[Category: Homo sapiens]] |
| - | [[Category: Arrowsmith, C H]] | + | [[Category: Large Structures]] |
| - | [[Category: Bountra, C]] | + | [[Category: Arrowsmith CH]] |
| - | [[Category: Edwards, A M]] | + | [[Category: Bountra C]] |
| - | [[Category: Liu, Y]] | + | [[Category: Edwards AM]] |
| - | [[Category: Min, J]] | + | [[Category: Liu Y]] |
| - | [[Category: Structural genomic]]
| + | [[Category: Min J]] |
| - | [[Category: Tempel, W]] | + | [[Category: Tempel W]] |
| - | [[Category: Crystal structure of the chromodomain of rbbp1]]
| + | |
| - | [[Category: Sgc]]
| + | |
| - | [[Category: Unknown function]]
| + | |
| Structural highlights
Function
ARI4A_HUMAN Interacts with the viral protein-binding domain of the retinoblastoma protein.
Publication Abstract from PubMed
RBBP1 is a retinoblastoma protein (pRb) binding protein acting as a repressor of gene transcription. RBBP1 is a multidomain protein including a chromo barrel domain, and its chromo barrel domain has been reported to recognize histone H4K20me3 weakly, and this binding is enhanced by the simultaneous binding of DNA. However, the molecular basis of this DNA-mediated histone binding by the chromo barrel domain of RBBP1 is unclear. Here we attempted to co-crystallize the chromo barrel domain of RBBP1 with either a histone H4K20me3 peptide alone or with both a histone H4K20me3 peptide and DNA, but only solved the peptide/DNA unbound crystal structure. Our structural analysis indicates that RBBP1 could interact with histone H4K20me3 similar to other histone binding chromo barrel domains, and the surface charge representation analysis of the chromo barrel domain of RBBP1 suggests that the chromo barrel domain of RBBP1 does not have a typical DNA binding surface, indicating that it might not bind to DNA. Consistently, our ITC assays also showed that DNA does not significantly enhance the histone binding ability of the chromo barrel domain of RBBP1.
Crystal structure of chromo barrel domain of RBBP1.,Lei M, Feng Y, Zhou M, Yang Y, Loppnau P, Li Y, Yang Y, Liu Y Biochem Biophys Res Commun. 2018 Feb 19;496(4):1344-1348. doi:, 10.1016/j.bbrc.2018.02.016. Epub 2018 Feb 3. PMID:29408527[1]
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.
References
- ↑ Lei M, Feng Y, Zhou M, Yang Y, Loppnau P, Li Y, Yang Y, Liu Y. Crystal structure of chromo barrel domain of RBBP1. Biochem Biophys Res Commun. 2018 Feb 19;496(4):1344-1348. doi:, 10.1016/j.bbrc.2018.02.016. Epub 2018 Feb 3. PMID:29408527 doi:http://dx.doi.org/10.1016/j.bbrc.2018.02.016
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