We apologize for Proteopedia being slow to respond. For the past two years, a new implementation of Proteopedia has been being built. Soon, it will replace this 18-year old system. All existing content will be moved to the new system at a date that will be announced here.

2ewt

From Proteopedia

(Difference between revisions)
Jump to: navigation, search
Current revision (13:50, 13 March 2024) (edit) (undo)
 
(13 intermediate revisions not shown.)
Line 1: Line 1:
-
[[Image:2ewt.gif|left|200px]]
 
-
{{Structure
+
==Crystal structure of the DNA-binding domain of BldD==
-
|PDB= 2ewt |SIZE=350|CAPTION= <scene name='initialview01'>2ewt</scene>, resolution 1.81&Aring;
+
<StructureSection load='2ewt' size='340' side='right'caption='[[2ewt]], [[Resolution|resolution]] 1.81&Aring;' scene=''>
-
|SITE=
+
== Structural highlights ==
-
|LIGAND= <scene name='pdbligand=SO4:SULFATE+ION'>SO4</scene>
+
<table><tr><td colspan='2'>[[2ewt]] is a 1 chain structure with sequence from [https://en.wikipedia.org/wiki/Streptomyces_coelicolor_A3(2) Streptomyces coelicolor A3(2)]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2EWT OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=2EWT FirstGlance]. <br>
-
|ACTIVITY=
+
</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution|Resolution]] 1.81&#8491;</td></tr>
-
|GENE=
+
<tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=SO4:SULFATE+ION'>SO4</scene></td></tr>
-
|DOMAIN=
+
<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=2ewt FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=2ewt OCA], [https://pdbe.org/2ewt PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=2ewt RCSB], [https://www.ebi.ac.uk/pdbsum/2ewt PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=2ewt ProSAT]</span></td></tr>
-
|RELATEDENTRY=
+
</table>
-
|RESOURCES=<span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=2ewt FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=2ewt OCA], [http://www.ebi.ac.uk/pdbsum/2ewt PDBsum], [http://www.rcsb.org/pdb/explore.do?structureId=2ewt RCSB]</span>
+
== Function ==
-
}}
+
[https://www.uniprot.org/uniprot/Q7AKQ8_STRCO Q7AKQ8_STRCO]
-
 
+
== Evolutionary Conservation ==
-
'''Crystal structure of the DNA-binding domain of BldD'''
+
[[Image:Consurf_key_small.gif|200px|right]]
-
 
+
Check<jmol>
-
 
+
<jmolCheckbox>
-
==Overview==
+
<scriptWhenChecked>; select protein; define ~consurf_to_do selected; consurf_initial_scene = true; script "/wiki/ConSurf/ew/2ewt_consurf.spt"</scriptWhenChecked>
-
BldD is a central regulator of the developmental process in Streptomyces coelicolor. The 1.8 angstroms resolution structure of the DNA-binding domain of BldD (BldDN) reveals that BldDN forms a compact globular domain composed of four helices (alpha1-alpha4) containing a helix-turn-helix motif (alpha2-alpha3) resembling that of the DNA-binding domain of lambda repressor. The BldDN/DNA complex model led us to design a series of mutants, which revealed the important role of alpha3 and the 'turn' region between alpha2 and alpha3 for DNA recognition. Based on the fact that BldD occupies two operator sites of bldN and whiG and shows significant disparity in the affinity toward the two operator sites when they are disconnected, we propose a model of cooperative binding, which means that the binding of one BldD dimer to the high affinity site facilitates that of the second BldD dimer to the low affinity site. In addition, structural and mutational investigation reveals that the Tyr62Cys mutation, found in the first-identified bldD mutant, can destabilize BldD structure by disrupting the hydrophobic core.
+
<scriptWhenUnchecked>script /wiki/extensions/Proteopedia/spt/initialview01.spt</scriptWhenUnchecked>
-
 
+
<text>to colour the structure by Evolutionary Conservation</text>
-
==About this Structure==
+
</jmolCheckbox>
-
2EWT is a [[Single protein]] structure of sequence from [http://en.wikipedia.org/wiki/Streptomyces_coelicolor Streptomyces coelicolor]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2EWT OCA].
+
</jmol>, as determined by [http://consurfdb.tau.ac.il/ ConSurfDB]. You may read the [[Conservation%2C_Evolutionary|explanation]] of the method and the full data available from [http://bental.tau.ac.il/new_ConSurfDB/main_output.php?pdb_ID=2ewt ConSurf].
-
 
+
<div style="clear:both"></div>
-
==Reference==
+
__TOC__
-
Crystal structure of the DNA-binding domain of BldD, a central regulator of aerial mycelium formation in Streptomyces coelicolor A3(2)., Kim IK, Lee CJ, Kim MK, Kim JM, Kim JH, Yim HS, Cha SS, Kang SO, Mol Microbiol. 2006 Jun;60(5):1179-93. PMID:[http://www.ncbi.nlm.nih.gov/pubmed/16689794 16689794]
+
</StructureSection>
-
[[Category: Single protein]]
+
[[Category: Large Structures]]
-
[[Category: Streptomyces coelicolor]]
+
[[Category: Cha SS]]
-
[[Category: Cha, S S.]]
+
[[Category: Kang SO]]
-
[[Category: Kang, S O.]]
+
[[Category: Kim IK]]
-
[[Category: Kim, I K.]]
+
[[Category: Kim JH]]
-
[[Category: Kim, J H.]]
+
[[Category: Kim JM]]
-
[[Category: Kim, J M.]]
+
[[Category: Kim MK]]
-
[[Category: Kim, M K.]]
+
[[Category: Lee CJ]]
-
[[Category: Lee, C J.]]
+
[[Category: Yim HS]]
-
[[Category: Yim, H S.]]
+
-
[[Category: the dna-binding domain of bldd]]
+
-
 
+
-
''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Mon Mar 31 02:54:51 2008''
+

Current revision

Crystal structure of the DNA-binding domain of BldD

PDB ID 2ewt

Drag the structure with the mouse to rotate

Proteopedia Page Contributors and Editors (what is this?)

OCA

Retrieved from "http://52.214.119.220/wiki/index.php/2ewt"
Personal tools