5zcn

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(New page: '''Unreleased structure''' The entry 5zcn is ON HOLD until Paper Publication Authors: Hemmi, H., Kodani, S., Miyake, Y., Kaweewan, I., Nakagawa, H. Description: Solution NMR structure ...)
Current revision (08:55, 9 October 2024) (edit) (undo)
 
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'''Unreleased structure'''
 
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The entry 5zcn is ON HOLD until Paper Publication
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==Solution NMR structure of a new lasso peptide brevunsin==
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<StructureSection load='5zcn' size='340' side='right'caption='[[5zcn]]' scene=''>
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== Structural highlights ==
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<table><tr><td colspan='2'>[[5zcn]] is a 1 chain structure with sequence from [https://en.wikipedia.org/wiki/Brevundimonas_diminuta Brevundimonas diminuta]. Full experimental information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=5ZCN OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=5ZCN FirstGlance]. <br>
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</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">Solution NMR, 15 models</td></tr>
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<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=5zcn FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=5zcn OCA], [https://pdbe.org/5zcn PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=5zcn RCSB], [https://www.ebi.ac.uk/pdbsum/5zcn PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=5zcn ProSAT]</span></td></tr>
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</table>
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<div style="background-color:#fffaf0;">
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== Publication Abstract from PubMed ==
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A shuttle vector pHSG396Sp was constructed to perform gene expression using Sphingomonas subterranea as a host. A new lasso peptide biosynthetic gene cluster, derived from Brevundimonas diminuta, was amplified by PCR and integrated to afford a expression vector pHSG396Sp-12697L. The new lasso peptide brevunsin was successfully produced by S. subterranea, harboring the expression vector, with a high production yield (10.2 mg from 1 L culture). The chemical structure of brevunsin was established by NMR and MS/MS experiments. Based on the information obtained from the NOE experiment, the three-dimensional structure of brevunsin was determined, which indicated that brevunsin possessed a typical lasso structure. This expression vector system provides a new heterologous production method for unexplored lasso peptides that are encoded by bacterial genomes.
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Authors: Hemmi, H., Kodani, S., Miyake, Y., Kaweewan, I., Nakagawa, H.
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Heterologous production of a new lasso peptide brevunsin in Sphingomonas subterranea.,Kodani S, Hemmi H, Miyake Y, Kaweewan I, Nakagawa H J Ind Microbiol Biotechnol. 2018 Nov;45(11):983-992. doi:, 10.1007/s10295-018-2077-6. Epub 2018 Sep 6. PMID:30191430<ref>PMID:30191430</ref>
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Description: Solution NMR structure of a new lasso peptide brevunsin
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From MEDLINE&reg;/PubMed&reg;, a database of the U.S. National Library of Medicine.<br>
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[[Category: Unreleased Structures]]
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</div>
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[[Category: Kodani, S]]
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<div class="pdbe-citations 5zcn" style="background-color:#fffaf0;"></div>
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[[Category: Hemmi, H]]
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== References ==
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[[Category: Kaweewan, I]]
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<references/>
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[[Category: Miyake, Y]]
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__TOC__
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[[Category: Nakagawa, H]]
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</StructureSection>
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[[Category: Brevundimonas diminuta]]
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[[Category: Large Structures]]
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[[Category: Hemmi H]]
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[[Category: Kaweewan I]]
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[[Category: Kodani S]]
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[[Category: Miyake Y]]
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[[Category: Nakagawa H]]

Current revision

Solution NMR structure of a new lasso peptide brevunsin

PDB ID 5zcn

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