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| | ==Bacteriophage Lambda Excisionase (Xis)-DNA Complex== | | ==Bacteriophage Lambda Excisionase (Xis)-DNA Complex== |
| - | <StructureSection load='1rh6' size='340' side='right' caption='[[1rh6]], [[Resolution|resolution]] 1.70Å' scene=''> | + | <StructureSection load='1rh6' size='340' side='right'caption='[[1rh6]], [[Resolution|resolution]] 1.70Å' scene=''> |
| | == Structural highlights == | | == Structural highlights == |
| - | <table><tr><td colspan='2'>[[1rh6]] is a 4 chain structure with sequence from [http://en.wikipedia.org/wiki/Bacteriophage_lambda Bacteriophage lambda]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1RH6 OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=1RH6 FirstGlance]. <br> | + | <table><tr><td colspan='2'>[[1rh6]] is a 4 chain structure with sequence from [https://en.wikipedia.org/wiki/Escherichia_virus_Lambda Escherichia virus Lambda]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1RH6 OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=1RH6 FirstGlance]. <br> |
| - | </td></tr><tr id='gene'><td class="sblockLbl"><b>[[Gene|Gene:]]</b></td><td class="sblockDat">xis ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=10710 Bacteriophage lambda])</td></tr> | + | </td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution|Resolution]] 1.7Å</td></tr> |
| - | <tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=1rh6 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1rh6 OCA], [http://pdbe.org/1rh6 PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=1rh6 RCSB], [http://www.ebi.ac.uk/pdbsum/1rh6 PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=1rh6 ProSAT]</span></td></tr> | + | <tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=1rh6 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1rh6 OCA], [https://pdbe.org/1rh6 PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=1rh6 RCSB], [https://www.ebi.ac.uk/pdbsum/1rh6 PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=1rh6 ProSAT]</span></td></tr> |
| | </table> | | </table> |
| | == Function == | | == Function == |
| - | [[http://www.uniprot.org/uniprot/VXIS_LAMBD VXIS_LAMBD]] Excisionase and integrase are necessary for the excision of prophage from the host genome by site-specific recombination at the att site. | + | [https://www.uniprot.org/uniprot/VXIS_LAMBD VXIS_LAMBD] Excisionase and integrase are necessary for the excision of prophage from the host genome by site-specific recombination at the att site. |
| - | <div style="background-color:#fffaf0;">
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| - | == Publication Abstract from PubMed ==
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| - | The excisionase (Xis) protein from bacteriophage lambda is the best characterized member of a large family of recombination directionality factors that control integrase-mediated DNA rearrangements. It triggers phage excision by cooperatively binding to sites X1 and X2 within the phage, bending DNA significantly and recruiting the phage-encoded integrase (Int) protein to site P2. We have determined the co-crystal structure of Xis with its X2 DNA-binding site at 1.7A resolution. Xis forms a unique winged-helix motif that interacts with the major and minor grooves of its binding site using an alpha-helix and an ordered beta-hairpin (wing), respectively. Recognition is achieved through an elaborate water-mediated hydrogen-bonding network at the major groove interface, while the preformed hairpin forms largely non-specific interactions with the minor groove. The structure of the complex provides insights into how Xis recruits Int cooperatively, and suggests a plausible mechanism by which it may distort longer DNA fragments significantly. It reveals a surface on the protein that is likely to mediate Xis-Xis interactions required for its cooperative binding to DNA.
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| - | Crystal structure of the excisionase-DNA complex from bacteriophage lambda.,Sam MD, Cascio D, Johnson RC, Clubb RT J Mol Biol. 2004 Apr 23;338(2):229-40. PMID:15066428<ref>PMID:15066428</ref>
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| - | From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br>
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| - | </div>
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| - | <div class="pdbe-citations 1rh6" style="background-color:#fffaf0;"></div>
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| - | == References ==
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| - | <references/>
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| | __TOC__ | | __TOC__ |
| | </StructureSection> | | </StructureSection> |
| - | [[Category: Bacteriophage lambda]] | + | [[Category: Escherichia virus Lambda]] |
| - | [[Category: Cascio, D]] | + | [[Category: Large Structures]] |
| - | [[Category: Clubb, R T]] | + | [[Category: Cascio D]] |
| - | [[Category: Johnson, R C]]
| + | [[Category: Clubb RT]] |
| - | [[Category: Sam, M D]]
| + | [[Category: Johnson RC]] |
| - | [[Category: Winged -helix protein]] | + | [[Category: Sam MD]] |
| - | [[Category: Dna architectural protein]] | + | |
| - | [[Category: Dna binding protein-dna complex]] | + | |
| - | [[Category: Phage excision]]
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| - | [[Category: Protein-dna complex]]
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| - | [[Category: Site-specific dna recombination]]
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