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| | ==NMR structure of the human dematin headpiece domain== | | ==NMR structure of the human dematin headpiece domain== |
| - | <StructureSection load='1qzp' size='340' side='right' caption='[[1qzp]], [[NMR_Ensembles_of_Models | 13 NMR models]]' scene=''> | + | <StructureSection load='1qzp' size='340' side='right'caption='[[1qzp]]' scene=''> |
| | == Structural highlights == | | == Structural highlights == |
| - | <table><tr><td colspan='2'>[[1qzp]] is a 1 chain structure with sequence from [http://en.wikipedia.org/wiki/Human Human]. Full experimental information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1QZP OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=1QZP FirstGlance]. <br> | + | <table><tr><td colspan='2'>[[1qzp]] is a 1 chain structure with sequence from [https://en.wikipedia.org/wiki/Homo_sapiens Homo sapiens]. Full experimental information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1QZP OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=1QZP FirstGlance]. <br> |
| - | </td></tr><tr id='related'><td class="sblockLbl"><b>[[Related_structure|Related:]]</b></td><td class="sblockDat">[[1qqv|1qqv]], [[1vii|1vii]]</td></tr> | + | </td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">Solution NMR</td></tr> |
| - | <tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=1qzp FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1qzp OCA], [http://pdbe.org/1qzp PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=1qzp RCSB], [http://www.ebi.ac.uk/pdbsum/1qzp PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=1qzp ProSAT]</span></td></tr> | + | <tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=1qzp FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1qzp OCA], [https://pdbe.org/1qzp PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=1qzp RCSB], [https://www.ebi.ac.uk/pdbsum/1qzp PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=1qzp ProSAT]</span></td></tr> |
| | </table> | | </table> |
| | == Function == | | == Function == |
| - | [[http://www.uniprot.org/uniprot/DEMA_HUMAN DEMA_HUMAN]] Actin-bundling protein. May function in mitogen-activated protein kinase pathway.<ref>PMID:11856323</ref> | + | [https://www.uniprot.org/uniprot/DEMA_HUMAN DEMA_HUMAN] Actin-bundling protein. May function in mitogen-activated protein kinase pathway.<ref>PMID:11856323</ref> |
| | == Evolutionary Conservation == | | == Evolutionary Conservation == |
| | [[Image:Consurf_key_small.gif|200px|right]] | | [[Image:Consurf_key_small.gif|200px|right]] |
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| | __TOC__ | | __TOC__ |
| | </StructureSection> | | </StructureSection> |
| - | [[Category: Human]] | + | [[Category: Homo sapiens]] |
| - | [[Category: Chishti, A H]] | + | [[Category: Large Structures]] |
| - | [[Category: Frank, B S]] | + | [[Category: Chishti AH]] |
| - | [[Category: McKnight, C J]] | + | [[Category: Frank BS]] |
| - | [[Category: Vardar, D]] | + | [[Category: McKnight CJ]] |
| - | [[Category: Actin binding domain]] | + | [[Category: Vardar D]] |
| - | [[Category: Dematin headpiece]]
| + | |
| - | [[Category: Protein binding]]
| + | |
| - | [[Category: Villin headpiece]]
| + | |
| Structural highlights
Function
DEMA_HUMAN Actin-bundling protein. May function in mitogen-activated protein kinase pathway.[1]
Evolutionary Conservation
Check, as determined by ConSurfDB. You may read the explanation of the method and the full data available from ConSurf.
Publication Abstract from PubMed
Dematin (band 4.9) is found in the junctional complex of the spectrin cytoskeleton that supports the erythrocyte cell membrane. Dematin is a member of the larger class of cytoskeleton-associated proteins that contain a modular "headpiece" domain at their extreme C termini. The dematin headpiece domain provides the second F-actin-binding site required for in vitro F-actin bundling. The dematin headpiece is found in two forms in the cell, one of 68 residues (DHP) and one containing a 22-amino acid insert near its N terminus (DHP+22). In addition, dematin contains the only headpiece domain that is phosphorylated, in vivo. The 22-amino acid insert in DHP+22 appeared unstructured in NMR spectra; therefore, we have determined the three-dimensional structure of DHP by multidimensional NMR methods. Although the overall three-dimensional structure of DHP is similar to that of the villin headpiece, there are two novel characteristics revealed by this structure. First, unlike villin headpiece that contains a single buried salt bridge, DHP contains a buried charged cluster comprising residues Glu(39), Arg(66), Lys(70), and the C-terminal carboxylate of Phe(76). Second, (15)N relaxation experiments indicate that the longer "variable loop" region near the N terminus of DHP (residues 20-29) is dynamic, undergoing significantly greater motions that the rest of the structure. Furthermore, NMR chemical shift changes indicate that the conformation of the dynamic variable loop is altered by phosphorylation of serine 74, which is far in the sequence from the variable loop region. Our results suggest that phosphorylation of the dematin headpiece acts as a conformational switch within this headpiece domain.
The NMR structure of dematin headpiece reveals a dynamic loop that is conformationally altered upon phosphorylation at a distal site.,Frank BS, Vardar D, Chishti AH, McKnight CJ J Biol Chem. 2004 Feb 27;279(9):7909-16. Epub 2003 Dec 2. PMID:14660664[2]
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.
References
- ↑ Lutchman M, Kim AC, Cheng L, Whitehead IP, Oh SS, Hanspal M, Boukharov AA, Hanada T, Chishti AH. Dematin interacts with the Ras-guanine nucleotide exchange factor Ras-GRF2 and modulates mitogen-activated protein kinase pathways. Eur J Biochem. 2002 Jan;269(2):638-49. PMID:11856323
- ↑ Frank BS, Vardar D, Chishti AH, McKnight CJ. The NMR structure of dematin headpiece reveals a dynamic loop that is conformationally altered upon phosphorylation at a distal site. J Biol Chem. 2004 Feb 27;279(9):7909-16. Epub 2003 Dec 2. PMID:14660664 doi:10.1074/jbc.M310524200
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