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2f5u

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[[Image:2f5u.gif|left|200px]]
 
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{{Structure
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==Structural Characterization of the UL25 DNA Packaging Protein from Herpes Simplex Virus Type 1==
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|PDB= 2f5u |SIZE=350|CAPTION= <scene name='initialview01'>2f5u</scene>, resolution 2.10&Aring;
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<StructureSection load='2f5u' size='340' side='right'caption='[[2f5u]], [[Resolution|resolution]] 2.10&Aring;' scene=''>
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|SITE=
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== Structural highlights ==
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|LIGAND=
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<table><tr><td colspan='2'>[[2f5u]] is a 1 chain structure with sequence from [https://en.wikipedia.org/wiki/Human_alphaherpesvirus_1 Human alphaherpesvirus 1]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2F5U OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=2F5U FirstGlance]. <br>
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|ACTIVITY=
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</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution|Resolution]] 2.1&#8491;</td></tr>
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|GENE= UL25 ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=10298 Human herpesvirus 1])
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<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=2f5u FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=2f5u OCA], [https://pdbe.org/2f5u PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=2f5u RCSB], [https://www.ebi.ac.uk/pdbsum/2f5u PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=2f5u ProSAT]</span></td></tr>
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|DOMAIN=
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</table>
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|RELATEDENTRY=
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== Function ==
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|RESOURCES=<span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=2f5u FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=2f5u OCA], [http://www.ebi.ac.uk/pdbsum/2f5u PDBsum], [http://www.rcsb.org/pdb/explore.do?structureId=2f5u RCSB]</span>
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[https://www.uniprot.org/uniprot/CVC2_HHV11 CVC2_HHV11] Capsid vertex-specific component that plays a role during viral DNA encapsidation, assuring correct genome cleavage and presumably stabilizing capsids that contain full-length viral genomes. Participates in the interaction between the capsid and the tegument through interaction with the large tegument protein/LTP. May mediate the capsid docking to the nuclear pore allowing entry of the viral genome into the host nucleus through binding to host nucleoporins NUP214.[HAMAP-Rule:MF_04025]<ref>PMID:18448531</ref> <ref>PMID:18945788</ref>
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}}
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== Evolutionary Conservation ==
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[[Image:Consurf_key_small.gif|200px|right]]
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'''Structural Characterization of the UL25 DNA Packaging Protein from Herpes Simplex Virus Type 1'''
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Check<jmol>
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<jmolCheckbox>
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<scriptWhenChecked>; select protein; define ~consurf_to_do selected; consurf_initial_scene = true; script "/wiki/ConSurf/f5/2f5u_consurf.spt"</scriptWhenChecked>
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==Overview==
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<scriptWhenUnchecked>script /wiki/extensions/Proteopedia/spt/initialview01.spt</scriptWhenUnchecked>
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Herpesviruses replicate their double stranded DNA genomes as high-molecular-weight concatemers which are subsequently cleaved into unit-length genomes by a complex mechanism that is tightly coupled to DNA insertion into a preformed capsid structure, the procapsid. The herpes simplex virus type 1 UL25 protein is incorporated into the capsid during DNA packaging, and previous studies of a null mutant have demonstrated that its function is essential at the late stages of the head-filling process, either to allow packaging to proceed to completion or for retention of the viral genome within the capsid. We have expressed and purified an N-terminally truncated form of the 580-residue UL25 protein and have determined the crystallographic structure of the region corresponding to amino acids 134 to 580 at 2.1-Angstroms resolution. This structure, the first for any herpesvirus protein involved in processing and packaging of viral DNA, reveals a novel fold, a distinctive electrostatic distribution, and a unique "flexible" architecture in which numerous flexible loops emanate from a stable core. Evolutionary trace analysis of UL25 and its homologues in other herpesviruses was used to locate potentially important amino acids on the surface of the protein, leading to the identification of four putative docking regions for protein partners.
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<text>to colour the structure by Evolutionary Conservation</text>
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</jmolCheckbox>
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==About this Structure==
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</jmol>, as determined by [http://consurfdb.tau.ac.il/ ConSurfDB]. You may read the [[Conservation%2C_Evolutionary|explanation]] of the method and the full data available from [http://bental.tau.ac.il/new_ConSurfDB/main_output.php?pdb_ID=2f5u ConSurf].
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2F5U is a [[Single protein]] structure of sequence from [http://en.wikipedia.org/wiki/Human_herpesvirus_1 Human herpesvirus 1]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2F5U OCA].
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<div style="clear:both"></div>
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== References ==
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==Reference==
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<references/>
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Structural characterization of the UL25 DNA-packaging protein from herpes simplex virus type 1., Bowman BR, Welschhans RL, Jayaram H, Stow ND, Preston VG, Quiocho FA, J Virol. 2006 Mar;80(5):2309-17. PMID:[http://www.ncbi.nlm.nih.gov/pubmed/16474137 16474137]
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__TOC__
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[[Category: Human herpesvirus 1]]
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</StructureSection>
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[[Category: Single protein]]
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[[Category: Human alphaherpesvirus 1]]
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[[Category: Bowman, B R.]]
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[[Category: Large Structures]]
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[[Category: capsid protein]]
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[[Category: Bowman BR]]
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[[Category: dna packaging]]
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[[Category: head completion]]
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[[Category: hsv-1]]
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[[Category: ul25]]
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''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Mon Mar 31 02:58:17 2008''
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Current revision

Structural Characterization of the UL25 DNA Packaging Protein from Herpes Simplex Virus Type 1

PDB ID 2f5u

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