1r5x

From Proteopedia

(Difference between revisions)
Jump to: navigation, search
Current revision (08:20, 14 February 2024) (edit) (undo)
 
(One intermediate revision not shown.)
Line 1: Line 1:
==JAMM: A Metalloprotease-like Zinc Site in the Proteasome and Signalosome==
==JAMM: A Metalloprotease-like Zinc Site in the Proteasome and Signalosome==
-
<StructureSection load='1r5x' size='340' side='right' caption='[[1r5x]], [[Resolution|resolution]] 2.30&Aring;' scene=''>
+
<StructureSection load='1r5x' size='340' side='right'caption='[[1r5x]], [[Resolution|resolution]] 2.30&Aring;' scene=''>
== Structural highlights ==
== Structural highlights ==
-
<table><tr><td colspan='2'>[[1r5x]] is a 2 chain structure with sequence from [http://en.wikipedia.org/wiki/Arcfu Arcfu]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1R5X OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=1R5X FirstGlance]. <br>
+
<table><tr><td colspan='2'>[[1r5x]] is a 2 chain structure with sequence from [https://en.wikipedia.org/wiki/Archaeoglobus_fulgidus_DSM_4304 Archaeoglobus fulgidus DSM 4304]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1R5X OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=1R5X FirstGlance]. <br>
-
</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=ZN:ZINC+ION'>ZN</scene></td></tr>
+
</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution|Resolution]] 2.3&#8491;</td></tr>
-
<tr id='gene'><td class="sblockLbl"><b>[[Gene|Gene:]]</b></td><td class="sblockDat">AF2198 ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=224325 ARCFU])</td></tr>
+
<tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=ZN:ZINC+ION'>ZN</scene></td></tr>
-
<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=1r5x FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1r5x OCA], [http://pdbe.org/1r5x PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=1r5x RCSB], [http://www.ebi.ac.uk/pdbsum/1r5x PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=1r5x ProSAT]</span></td></tr>
+
<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=1r5x FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1r5x OCA], [https://pdbe.org/1r5x PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=1r5x RCSB], [https://www.ebi.ac.uk/pdbsum/1r5x PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=1r5x ProSAT]</span></td></tr>
</table>
</table>
 +
== Function ==
 +
[https://www.uniprot.org/uniprot/O28085_ARCFU O28085_ARCFU]
== Evolutionary Conservation ==
== Evolutionary Conservation ==
[[Image:Consurf_key_small.gif|200px|right]]
[[Image:Consurf_key_small.gif|200px|right]]
Line 18: Line 20:
</jmol>, as determined by [http://consurfdb.tau.ac.il/ ConSurfDB]. You may read the [[Conservation%2C_Evolutionary|explanation]] of the method and the full data available from [http://bental.tau.ac.il/new_ConSurfDB/main_output.php?pdb_ID=1r5x ConSurf].
</jmol>, as determined by [http://consurfdb.tau.ac.il/ ConSurfDB]. You may read the [[Conservation%2C_Evolutionary|explanation]] of the method and the full data available from [http://bental.tau.ac.il/new_ConSurfDB/main_output.php?pdb_ID=1r5x ConSurf].
<div style="clear:both"></div>
<div style="clear:both"></div>
-
<div style="background-color:#fffaf0;">
 
-
== Publication Abstract from PubMed ==
 
-
The JAMM (JAB1/MPN/Mov34 metalloenzyme) motif in Rpn11 and Csn5 underlies isopeptidase activities intrinsic to the proteasome and signalosome, respectively. We show here that the archaebacterial protein AfJAMM possesses the key features of a zinc metalloprotease, yet with a distinct fold. The histidine and aspartic acid of the conserved EX(n)HS/THX(7)SXXD motif coordinate a zinc, whereas the glutamic acid hydrogen-bonds an aqua ligand. By analogy to the active site of thermolysin, we predict that the glutamic acid serves as an acid-base catalyst and the second serine stabilizes a tetrahedral intermediate. Mutagenesis of Csn5 confirms these residues are required for Nedd8 isopeptidase activity. The active site-like architecture specified by the JAMM motif motivates structure-based approaches to the study of JAMM domain proteins and the development of therapeutic proteasome and signalosome inhibitors.
 
- 
-
JAMM: a metalloprotease-like zinc site in the proteasome and signalosome.,Ambroggio XI, Rees DC, Deshaies RJ PLoS Biol. 2004 Jan;2(1):E2. Epub 2003 Nov 24. PMID:14737182<ref>PMID:14737182</ref>
 
- 
-
From MEDLINE&reg;/PubMed&reg;, a database of the U.S. National Library of Medicine.<br>
 
-
</div>
 
-
<div class="pdbe-citations 1r5x" style="background-color:#fffaf0;"></div>
 
-
== References ==
 
-
<references/>
 
__TOC__
__TOC__
</StructureSection>
</StructureSection>
-
[[Category: Arcfu]]
+
[[Category: Archaeoglobus fulgidus DSM 4304]]
-
[[Category: Ambroggio, X I]]
+
[[Category: Large Structures]]
-
[[Category: Deshaies, R J]]
+
[[Category: Ambroggio XI]]
-
[[Category: Rees, D C]]
+
[[Category: Deshaies RJ]]
-
[[Category: Csn5]]
+
[[Category: Rees DC]]
-
[[Category: Jab1]]
+
-
[[Category: Jamm]]
+
-
[[Category: Mov34]]
+
-
[[Category: Mpn]]
+
-
[[Category: Proteasome]]
+
-
[[Category: Rpn11]]
+
-
[[Category: Signalosome]]
+
-
[[Category: Structural genomic]]
+
-
[[Category: Unknown function]]
+

Current revision

JAMM: A Metalloprotease-like Zinc Site in the Proteasome and Signalosome

PDB ID 1r5x

Drag the structure with the mouse to rotate

Proteopedia Page Contributors and Editors (what is this?)

OCA

Personal tools