6ck5
From Proteopedia
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| - | '''Unreleased structure''' | ||
| - | + | ==PRPP riboswitch from T. mathranii bound to PRPP== | |
| + | <StructureSection load='6ck5' size='340' side='right'caption='[[6ck5]], [[Resolution|resolution]] 2.49Å' scene=''> | ||
| + | == Structural highlights == | ||
| + | <table><tr><td colspan='2'>[[6ck5]] is a 2 chain structure with sequence from [https://en.wikipedia.org/wiki/Thermoanaerobacter_mathranii Thermoanaerobacter mathranii]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=6CK5 OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=6CK5 FirstGlance]. <br> | ||
| + | </td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution|Resolution]] 2.49Å</td></tr> | ||
| + | <tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=BA:BARIUM+ION'>BA</scene>, <scene name='pdbligand=K:POTASSIUM+ION'>K</scene>, <scene name='pdbligand=MG:MAGNESIUM+ION'>MG</scene>, <scene name='pdbligand=PRP:ALPHA-PHOSPHORIBOSYLPYROPHOSPHORIC+ACID'>PRP</scene></td></tr> | ||
| + | <tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=6ck5 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=6ck5 OCA], [https://pdbe.org/6ck5 PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=6ck5 RCSB], [https://www.ebi.ac.uk/pdbsum/6ck5 PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=6ck5 ProSAT]</span></td></tr> | ||
| + | </table> | ||
| + | <div style="background-color:#fffaf0;"> | ||
| + | == Publication Abstract from PubMed == | ||
| + | Two classes of riboswitches related to the ykkC guanidine-I riboswitch bind phosphoribosyl pyrophosphate (PRPP) and guanosine tetraphosphate (ppGpp). Here we report the co-crystal structure of the PRPP aptamer and its ligand. We also report the structure of the G96A point mutant that prefers ppGpp over PRPP with a dramatic 40,000-fold switch in specificity. The ends of the aptamer form a helix that is not present in the guanidine aptamer and is involved in the expression platform. In the mutant, the base of ppGpp replaces G96 in three-dimensional space. This disrupts the S-turn, which is a primary structural feature of the ykkC RNA motif. These dramatic differences in ligand specificity are achieved with minimal mutations. ykkC aptamers are therefore a prime example of an RNA fold with a rugged fitness landscape. The ease with which the ykkC aptamer acquires new specificity represents a striking case of evolvability in RNA. | ||
| - | + | Structures of two aptamers with differing ligand specificity reveal ruggedness in the functional landscape of RNA.,Knappenberger AJ, Reiss CW, Strobel SA Elife. 2018 Jun 7;7. pii: 36381. doi: 10.7554/eLife.36381. PMID:29877798<ref>PMID:29877798</ref> | |
| - | + | From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br> | |
| - | [[Category: | + | </div> |
| + | <div class="pdbe-citations 6ck5" style="background-color:#fffaf0;"></div> | ||
| + | |||
| + | ==See Also== | ||
| + | *[[Riboswitch 3D structures|Riboswitch 3D structures]] | ||
| + | == References == | ||
| + | <references/> | ||
| + | __TOC__ | ||
| + | </StructureSection> | ||
| + | [[Category: Large Structures]] | ||
| + | [[Category: Thermoanaerobacter mathranii]] | ||
| + | [[Category: Knappenberger AJ]] | ||
| + | [[Category: Reiss CW]] | ||
| + | [[Category: Strobel SA]] | ||
Current revision
PRPP riboswitch from T. mathranii bound to PRPP
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