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| | ==Structure of two RcsB dimers bound to two parallel DNAs.== | | ==Structure of two RcsB dimers bound to two parallel DNAs.== |
| - | <StructureSection load='5vxn' size='340' side='right' caption='[[5vxn]], [[Resolution|resolution]] 3.38Å' scene=''> | + | <StructureSection load='5vxn' size='340' side='right'caption='[[5vxn]], [[Resolution|resolution]] 3.38Å' scene=''> |
| | == Structural highlights == | | == Structural highlights == |
| - | <table><tr><td colspan='2'>[[5vxn]] is a 8 chain structure. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=5VXN OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=5VXN FirstGlance]. <br> | + | <table><tr><td colspan='2'>[[5vxn]] is a 8 chain structure with sequence from [https://en.wikipedia.org/wiki/Escherichia_coli Escherichia coli] and [https://en.wikipedia.org/wiki/Escherichia_coli_K-12 Escherichia coli K-12]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=5VXN OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=5VXN FirstGlance]. <br> |
| - | </td></tr><tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=5vxn FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=5vxn OCA], [http://pdbe.org/5vxn PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=5vxn RCSB], [http://www.ebi.ac.uk/pdbsum/5vxn PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=5vxn ProSAT]</span></td></tr> | + | </td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution|Resolution]] 3.375Å</td></tr> |
| | + | <tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=5vxn FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=5vxn OCA], [https://pdbe.org/5vxn PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=5vxn RCSB], [https://www.ebi.ac.uk/pdbsum/5vxn PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=5vxn ProSAT]</span></td></tr> |
| | </table> | | </table> |
| | == Function == | | == Function == |
| - | [[http://www.uniprot.org/uniprot/RCSB_ECOLI RCSB_ECOLI]] Component of the Rcs signaling system, which controls transcription of numerous genes. RcsB is the response regulator that binds to regulatory DNA regions. Can function both in an RcsA-dependent or RcsA-independent manner. The system regulates expression of numerous genes, including genes involved in colanic acid capsule synthesis, biofilm formation, cell division and outer membrane proteins synthesis. Also involved, with GadE, in control of glutamate-dependent acid resistance, and, with BglJ, in derepression of the cryptic bgl operon. The RcsB-BglJ activity is probably independent of RcsB phosphorylation.[HAMAP-Rule:MF_00981]<ref>PMID:10702265</ref> <ref>PMID:11309126</ref> <ref>PMID:11566985</ref> <ref>PMID:13129944</ref> <ref>PMID:1597415</ref> <ref>PMID:20189963</ref> <ref>PMID:20952573</ref> | + | [https://www.uniprot.org/uniprot/RCSB_ECOLI RCSB_ECOLI] Component of the Rcs signaling system, which controls transcription of numerous genes. RcsB is the response regulator that binds to regulatory DNA regions. Can function both in an RcsA-dependent or RcsA-independent manner. The system regulates expression of numerous genes, including genes involved in colanic acid capsule synthesis, biofilm formation, cell division and outer membrane proteins synthesis. Also involved, with GadE, in control of glutamate-dependent acid resistance, and, with BglJ, in derepression of the cryptic bgl operon. The RcsB-BglJ activity is probably independent of RcsB phosphorylation.[HAMAP-Rule:MF_00981]<ref>PMID:10702265</ref> <ref>PMID:11309126</ref> <ref>PMID:11566985</ref> <ref>PMID:13129944</ref> <ref>PMID:1597415</ref> <ref>PMID:20189963</ref> <ref>PMID:20952573</ref> |
| | == References == | | == References == |
| | <references/> | | <references/> |
| | __TOC__ | | __TOC__ |
| | </StructureSection> | | </StructureSection> |
| - | [[Category: Anderson, W F]] | + | [[Category: Escherichia coli]] |
| - | [[Category: Structural genomic]] | + | [[Category: Escherichia coli K-12]] |
| - | [[Category: Filippova, E V]] | + | [[Category: Large Structures]] |
| - | [[Category: Minasov, G]] | + | [[Category: Anderson WF]] |
| - | [[Category: Pshenychnyi, S]] | + | [[Category: Filippova EV]] |
| - | [[Category: Csgid]] | + | [[Category: Minasov G]] |
| - | [[Category: Dna binding protein-dna complex]] | + | [[Category: Pshenychnyi S]] |
| - | [[Category: Dna-binding protein]]
| + | |
| - | [[Category: Fixj/narl]]
| + | |
| - | [[Category: Rcs phosphorelay]]
| + | |
| - | [[Category: Response regulator]]
| + | |
| Structural highlights
Function
RCSB_ECOLI Component of the Rcs signaling system, which controls transcription of numerous genes. RcsB is the response regulator that binds to regulatory DNA regions. Can function both in an RcsA-dependent or RcsA-independent manner. The system regulates expression of numerous genes, including genes involved in colanic acid capsule synthesis, biofilm formation, cell division and outer membrane proteins synthesis. Also involved, with GadE, in control of glutamate-dependent acid resistance, and, with BglJ, in derepression of the cryptic bgl operon. The RcsB-BglJ activity is probably independent of RcsB phosphorylation.[HAMAP-Rule:MF_00981][1] [2] [3] [4] [5] [6] [7]
References
- ↑ Wehland M, Bernhard F. The RcsAB box. Characterization of a new operator essential for the regulation of exopolysaccharide biosynthesis in enteric bacteria. J Biol Chem. 2000 Mar 10;275(10):7013-20. PMID:10702265
- ↑ Takeda S, Fujisawa Y, Matsubara M, Aiba H, Mizuno T. A novel feature of the multistep phosphorelay in Escherichia coli: a revised model of the RcsC --> YojN --> RcsB signalling pathway implicated in capsular synthesis and swarming behaviour. Mol Microbiol. 2001 Apr;40(2):440-50. PMID:11309126
- ↑ Davalos-Garcia M, Conter A, Toesca I, Gutierrez C, Cam K. Regulation of osmC gene expression by the two-component system rcsB-rcsC in Escherichia coli. J Bacteriol. 2001 Oct;183(20):5870-6. PMID:11566985 doi:http://dx.doi.org/10.1128/JB.***********-5876.2001
- ↑ Hagiwara D, Sugiura M, Oshima T, Mori H, Aiba H, Yamashino T, Mizuno T. Genome-wide analyses revealing a signaling network of the RcsC-YojN-RcsB phosphorelay system in Escherichia coli. J Bacteriol. 2003 Oct;185(19):5735-46. PMID:13129944
- ↑ Gervais FG, Phoenix P, Drapeau GR. The rcsB gene, a positive regulator of colanic acid biosynthesis in Escherichia coli, is also an activator of ftsZ expression. J Bacteriol. 1992 Jun;174(12):3964-71. PMID:1597415
- ↑ Castanie-Cornet MP, Cam K, Bastiat B, Cros A, Bordes P, Gutierrez C. Acid stress response in Escherichia coli: mechanism of regulation of gadA transcription by RcsB and GadE. Nucleic Acids Res. 2010 Jun;38(11):3546-54. doi: 10.1093/nar/gkq097. Epub 2010, Feb 26. PMID:20189963 doi:http://dx.doi.org/10.1093/nar/gkq097
- ↑ Venkatesh GR, Kembou Koungni FC, Paukner A, Stratmann T, Blissenbach B, Schnetz K. BglJ-RcsB heterodimers relieve repression of the Escherichia coli bgl operon by H-NS. J Bacteriol. 2010 Dec;192(24):6456-64. doi: 10.1128/JB.00807-10. Epub 2010 Oct, 15. PMID:20952573 doi:http://dx.doi.org/10.1128/JB.00807-10
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