This old version of Proteopedia is provided for student assignments while the new version is undergoing repairs. Content and edits done in this old version of Proteopedia after March 1, 2026 will eventually be lost when it is retired in about June of 2026.

Apply for new accounts at the new Proteopedia. Your logins will work in both the old and new versions.


4qi6

From Proteopedia

(Difference between revisions)
Jump to: navigation, search
Current revision (06:52, 3 April 2024) (edit) (undo)
 
(2 intermediate revisions not shown.)
Line 1: Line 1:
==Cellobiose dehydrogenase from Myriococcum thermophilum, MtCDH==
==Cellobiose dehydrogenase from Myriococcum thermophilum, MtCDH==
-
<StructureSection load='4qi6' size='340' side='right' caption='[[4qi6]], [[Resolution|resolution]] 3.20&Aring;' scene=''>
+
<StructureSection load='4qi6' size='340' side='right'caption='[[4qi6]], [[Resolution|resolution]] 3.20&Aring;' scene=''>
== Structural highlights ==
== Structural highlights ==
-
<table><tr><td colspan='2'>[[4qi6]] is a 1 chain structure with sequence from [http://en.wikipedia.org/wiki/Atcc_22112 Atcc 22112]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=4QI6 OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=4QI6 FirstGlance]. <br>
+
<table><tr><td colspan='2'>[[4qi6]] is a 1 chain structure with sequence from [https://en.wikipedia.org/wiki/Crassicarpon_hotsonii Crassicarpon hotsonii]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=4QI6 OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=4QI6 FirstGlance]. <br>
-
</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=FAD:FLAVIN-ADENINE+DINUCLEOTIDE'>FAD</scene>, <scene name='pdbligand=HEM:PROTOPORPHYRIN+IX+CONTAINING+FE'>HEM</scene>, <scene name='pdbligand=MAN:ALPHA-D-MANNOSE'>MAN</scene>, <scene name='pdbligand=NAG:N-ACETYL-D-GLUCOSAMINE'>NAG</scene></td></tr>
+
</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution|Resolution]] 3.2&#8491;</td></tr>
-
<tr id='NonStdRes'><td class="sblockLbl"><b>[[Non-Standard_Residue|NonStd Res:]]</b></td><td class="sblockDat"><scene name='pdbligand=PCA:PYROGLUTAMIC+ACID'>PCA</scene></td></tr>
+
<tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=FAD:FLAVIN-ADENINE+DINUCLEOTIDE'>FAD</scene>, <scene name='pdbligand=HEM:PROTOPORPHYRIN+IX+CONTAINING+FE'>HEM</scene>, <scene name='pdbligand=MAN:ALPHA-D-MANNOSE'>MAN</scene>, <scene name='pdbligand=NAG:N-ACETYL-D-GLUCOSAMINE'>NAG</scene>, <scene name='pdbligand=PCA:PYROGLUTAMIC+ACID'>PCA</scene></td></tr>
-
<tr id='related'><td class="sblockLbl"><b>[[Related_structure|Related:]]</b></td><td class="sblockDat">[[4qi3|4qi3]], [[4qi4|4qi4]], [[4qi5|4qi5]], [[4qi7|4qi7]], [[4qi8|4qi8]]</td></tr>
+
<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=4qi6 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=4qi6 OCA], [https://pdbe.org/4qi6 PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=4qi6 RCSB], [https://www.ebi.ac.uk/pdbsum/4qi6 PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=4qi6 ProSAT]</span></td></tr>
-
<tr id='gene'><td class="sblockLbl"><b>[[Gene|Gene:]]</b></td><td class="sblockDat">CDH ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=455373 ATCC 22112])</td></tr>
+
-
<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=4qi6 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=4qi6 OCA], [http://pdbe.org/4qi6 PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=4qi6 RCSB], [http://www.ebi.ac.uk/pdbsum/4qi6 PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=4qi6 ProSAT]</span></td></tr>
+
</table>
</table>
-
<div style="background-color:#fffaf0;">
+
== Function ==
-
== Publication Abstract from PubMed ==
+
[https://www.uniprot.org/uniprot/A9XK88_9PEZI A9XK88_9PEZI]
-
A new paradigm for cellulose depolymerization by fungi focuses on an oxidative mechanism involving cellobiose dehydrogenases (CDH) and copper-dependent lytic polysaccharide monooxygenases (LPMO); however, mechanistic studies have been hampered by the lack of structural information regarding CDH. CDH contains a haem-binding cytochrome (CYT) connected via a flexible linker to a flavin-dependent dehydrogenase (DH). Electrons are generated from cellobiose oxidation catalysed by DH and shuttled via CYT to LPMO. Here we present structural analyses that provide a comprehensive picture of CDH conformers, which govern the electron transfer between redox centres. Using structure-based site-directed mutagenesis, rapid kinetics analysis and molecular docking, we demonstrate that flavin-to-haem interdomain electron transfer (IET) is enabled by a haem propionate group and that rapid IET requires a closed CDH state in which the propionate is tightly enfolded by DH. Following haem reduction, CYT reduces LPMO to initiate oxygen activation at the copper centre and subsequent cellulose depolymerization.
+
-
 
+
-
Structural basis for cellobiose dehydrogenase action during oxidative cellulose degradation.,Tan TC, Kracher D, Gandini R, Sygmund C, Kittl R, Haltrich D, Hallberg BM, Ludwig R, Divne C Nat Commun. 2015 Jul 7;6:7542. doi: 10.1038/ncomms8542. PMID:26151670<ref>PMID:26151670</ref>
+
-
 
+
-
From MEDLINE&reg;/PubMed&reg;, a database of the U.S. National Library of Medicine.<br>
+
-
</div>
+
-
<div class="pdbe-citations 4qi6" style="background-color:#fffaf0;"></div>
+
-
== References ==
+
-
<references/>
+
__TOC__
__TOC__
</StructureSection>
</StructureSection>
-
[[Category: Atcc 22112]]
+
[[Category: Crassicarpon hotsonii]]
-
[[Category: Divne, C]]
+
[[Category: Large Structures]]
-
[[Category: Gandini, R]]
+
[[Category: Divne C]]
-
[[Category: Hallberg, B M]]
+
[[Category: Gandini R]]
-
[[Category: Haltrich, D]]
+
[[Category: Hallberg BM]]
-
[[Category: Kittl, R]]
+
[[Category: Haltrich D]]
-
[[Category: Ludwig, R]]
+
[[Category: Kittl R]]
-
[[Category: Sygmund, C]]
+
[[Category: Ludwig R]]
-
[[Category: Tan, T C]]
+
[[Category: Sygmund C]]
-
[[Category: Cellobiose]]
+
[[Category: Tan TC]]
-
[[Category: Cellobiose oxidizing]]
+
-
[[Category: Electron transfer]]
+
-
[[Category: Lignocellulose degradation]]
+
-
[[Category: Lpmo]]
+
-
[[Category: Oxidoreductase]]
+

Current revision

Cellobiose dehydrogenase from Myriococcum thermophilum, MtCDH

PDB ID 4qi6

Drag the structure with the mouse to rotate

Proteopedia Page Contributors and Editors (what is this?)

OCA

Retrieved from "http://52.214.119.220/wiki/index.php/4qi6"
Personal tools