5c05

From Proteopedia

(Difference between revisions)

Current revision

Crystal Structure of Gamma-terpinene Synthase from Thymus vulgaris

Structural highlights

5c05 is a 2 chain structure with sequence from Thymus vulgaris. Full crystallographic information is available from OCA. For a guided tour on the structure components use FirstGlance.
Method:	X-ray diffraction, Resolution 1.65Å
Ligands:
Resources:	FirstGlance, OCA, PDBe, RCSB, PDBsum, ProSAT

Function

GTPS1_THYVU Involved in the biosynthesis of phenolic monoterpenes natural products thymol and carvacrol which have a broad range of biological activities acting as antimicrobial compounds, insecticides, antioxidants and pharmaceutical agents (PubMed:26750479). Monoterpene synthase which catalyzes the conversion of geranyl diphosphate (GPP) to gamma-terpinene and the minor products alpha-thujene, alpha-terpinene, myrcene, sabinene, (+)-R-limonene, alpha-pinene and alpha-phellandrene (PubMed:26750479).^[1]

Publication Abstract from PubMed

The biosynthesis of gamma-terpinene, a precursor of the phenolic isomers thymol and carvacrol found in the essential oil from Thymus sp., is attributed to the activitiy of gamma-terpinene synthase (TPS). Purified gamma-terpinene synthase from T. vulgaris (TvTPS), the Thymus species that is the most widely spread and of the greatest economical importance, is able to catalyze the enzymatic conversion of geranyl diphosphate (GPP) to gamma-terpinene. The crystal structure of recombinantly expressed and purified TvTPS is reported at 1.65 A resolution, confirming the dimeric structure of the enzyme. The putative active site of TvTPS is deduced from its pronounced structural similarity to enzymes from other species of the Lamiaceae family involved in terpenoid biosynthesis: to (+)-bornyl diphosphate synthase and 1,8-cineole synthase from Salvia sp. and to (4S)-limonene synthase from Mentha spicata.

Expression, crystallization and structure elucidation of gamma-terpinene synthase from Thymus vulgaris.,Rudolph K, Parthier C, Egerer-Sieber C, Geiger D, Muller YA, Kreis W, Muller-Uri F Acta Crystallogr F Struct Biol Commun. 2016 Jan 1;72(Pt 1):16-23. doi:, 10.1107/S2053230X15023043. Epub 2016 Jan 1. PMID:26750479^[2]

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.

References

↑ Rudolph K, Parthier C, Egerer-Sieber C, Geiger D, Muller YA, Kreis W, Muller-Uri F. Expression, crystallization and structure elucidation of gamma-terpinene synthase from Thymus vulgaris. Acta Crystallogr F Struct Biol Commun. 2016 Jan 1;72(Pt 1):16-23. doi:, 10.1107/S2053230X15023043. Epub 2016 Jan 1. PMID:26750479 doi:http://dx.doi.org/10.1107/S2053230X15023043
↑ Rudolph K, Parthier C, Egerer-Sieber C, Geiger D, Muller YA, Kreis W, Muller-Uri F. Expression, crystallization and structure elucidation of gamma-terpinene synthase from Thymus vulgaris. Acta Crystallogr F Struct Biol Commun. 2016 Jan 1;72(Pt 1):16-23. doi:, 10.1107/S2053230X15023043. Epub 2016 Jan 1. PMID:26750479 doi:http://dx.doi.org/10.1107/S2053230X15023043

1 Structural highlights
2 Function
3 Publication Abstract from PubMed
4 References

Proteopedia Page Contributors and Editors (what is this?)

OCA

Retrieved from "http://52.214.119.220/wiki/index.php/5c05"

@@ Line 1: / Line 1: @@
 ==Crystal Structure of Gamma-terpinene Synthase from Thymus vulgaris==
-<StructureSection load='5c05' size='340' side='right' caption='[[5c05]], [[Resolution|resolution]] 1.65&Aring;' scene=''>
+<StructureSection load='5c05' size='340' side='right'caption='[[5c05]], [[Resolution|resolution]] 1.65&Aring;' scene=''>
 == Structural highlights ==
-<table><tr><td colspan='2'>[[5c05]] is a 2 chain structure with sequence from [http://en.wikipedia.org/wiki/Thyvu Thyvu]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=5C05 OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=5C05 FirstGlance]. <br>
+<table><tr><td colspan='2'>[[5c05]] is a 2 chain structure with sequence from [https://en.wikipedia.org/wiki/Thymus_vulgaris Thymus vulgaris]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=5C05 OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=5C05 FirstGlance]. <br>
-</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=EDO:1,2-ETHANEDIOL'>EDO</scene></td></tr>
+</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution|Resolution]] 1.65&#8491;</td></tr>
-<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=5c05 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=5c05 OCA], [http://pdbe.org/5c05 PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=5c05 RCSB], [http://www.ebi.ac.uk/pdbsum/5c05 PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=5c05 ProSAT]</span></td></tr>
+<tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=EDO:1,2-ETHANEDIOL'>EDO</scene></td></tr>
+<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=5c05 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=5c05 OCA], [https://pdbe.org/5c05 PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=5c05 RCSB], [https://www.ebi.ac.uk/pdbsum/5c05 PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=5c05 ProSAT]</span></td></tr>
 </table>
+== Function ==
+[https://www.uniprot.org/uniprot/GTPS1_THYVU GTPS1_THYVU] Involved in the biosynthesis of phenolic monoterpenes natural products thymol and carvacrol which have a broad range of biological activities acting as antimicrobial compounds, insecticides, antioxidants and pharmaceutical agents (PubMed:26750479). Monoterpene synthase which catalyzes the conversion of geranyl diphosphate (GPP) to gamma-terpinene and the minor products alpha-thujene, alpha-terpinene, myrcene, sabinene, (+)-R-limonene, alpha-pinene and alpha-phellandrene (PubMed:26750479).<ref>PMID:26750479</ref>
 <div style="background-color:#fffaf0;">
 == Publication Abstract from PubMed ==
@@ Line 20: / Line 23: @@
 __TOC__
 </StructureSection>
-[[Category: Thyvu]]
+[[Category: Large Structures]]
-[[Category: Mueller-Uri, F]]
+[[Category: Thymus vulgaris]]
-[[Category: Muller, Y A]]
+[[Category: Mueller-Uri F]]
-[[Category: Parthier, C P]]
+[[Category: Muller YA]]
-[[Category: Rudolph, K]]
+[[Category: Parthier CP]]
-[[Category: Biosynthetic protein]]
+[[Category: Rudolph K]]
-[[Category: Gamma-terpinene]]
-[[Category: Plant]]
-[[Category: Plant protein]]
-[[Category: Terpene]]
-[[Category: Terpenoid synthesis]]

5c05

From Proteopedia

Current revision

Crystal Structure of Gamma-terpinene Synthase from Thymus vulgaris

Structural highlights

Function

Publication Abstract from PubMed

References

Contents

Proteopedia Page Contributors and Editors (what is this?)

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