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1sfu
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==Crystal structure of the viral Zalpha domain bound to left-handed Z-DNA== | ==Crystal structure of the viral Zalpha domain bound to left-handed Z-DNA== | ||
| - | <StructureSection load='1sfu' size='340' side='right' caption='[[1sfu]], [[Resolution|resolution]] 2.00Å' scene=''> | + | <StructureSection load='1sfu' size='340' side='right'caption='[[1sfu]], [[Resolution|resolution]] 2.00Å' scene=''> |
== Structural highlights == | == Structural highlights == | ||
| - | <table><tr><td colspan='2'>[[1sfu]] is a 4 chain structure with sequence from [ | + | <table><tr><td colspan='2'>[[1sfu]] is a 4 chain structure with sequence from [https://en.wikipedia.org/wiki/Yaba-like_disease_virus Yaba-like disease virus]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1SFU OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=1SFU FirstGlance]. <br> |
| - | </td></tr><tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[ | + | </td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution|Resolution]] 2Å</td></tr> |
| + | <tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=1sfu FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1sfu OCA], [https://pdbe.org/1sfu PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=1sfu RCSB], [https://www.ebi.ac.uk/pdbsum/1sfu PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=1sfu ProSAT]</span></td></tr> | ||
</table> | </table> | ||
| + | == Function == | ||
| + | [https://www.uniprot.org/uniprot/E3_YLDV E3_YLDV] RNA-binding protein that plays a role in the inhibition of multiple cellular antiviral responses activated by double-stranded RNA (dsRNA), such as inhibition of PKR activation, necroptosis, and IFN-mediated antiviral activities (By similarity). Recognizes and binds Z-RNA structures via its Z-binding domain and dsRNA via its DRBM domain: RNA-binding activity is required to escape host ZBP1-dependent necroptosis (By similarity). Mechanistically, the Z-binding domain binds Z-RNAs that are produced during Yaba-like disease virus infection, thereby competing with Z-RNA detection by host ZBP1, suppressing ZBP1-dependent necroptosis (By similarity).[UniProtKB:P21605] | ||
== Evolutionary Conservation == | == Evolutionary Conservation == | ||
[[Image:Consurf_key_small.gif|200px|right]] | [[Image:Consurf_key_small.gif|200px|right]] | ||
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</jmol>, as determined by [http://consurfdb.tau.ac.il/ ConSurfDB]. You may read the [[Conservation%2C_Evolutionary|explanation]] of the method and the full data available from [http://bental.tau.ac.il/new_ConSurfDB/main_output.php?pdb_ID=1sfu ConSurf]. | </jmol>, as determined by [http://consurfdb.tau.ac.il/ ConSurfDB]. You may read the [[Conservation%2C_Evolutionary|explanation]] of the method and the full data available from [http://bental.tau.ac.il/new_ConSurfDB/main_output.php?pdb_ID=1sfu ConSurf]. | ||
<div style="clear:both"></div> | <div style="clear:both"></div> | ||
| - | <div style="background-color:#fffaf0;"> | ||
| - | == Publication Abstract from PubMed == | ||
| - | A conserved feature of poxviruses is a protein, well characterized as E3L in vaccinia virus, that confers IFN resistance on the virus. This protein comprises two domains, an N-terminal Z-DNA-binding protein domain (Zalpha) and a C-terminal double-stranded RNA-binding domain. Both are required for pathogenicity of vaccinia virus in mice infected by intracranial injection. Here, we describe the crystal structure of the Zalpha domain from the E3L-like protein of Yaba-like disease virus, a Yatapoxvirus, in a complex with Z-DNA, solved at a 2.0-A resolution. The DNA contacting surface of Yaba-like disease virus Zalpha(E3L) closely resembles that of other structurally defined members of the Zalpha family, although some variability exists in the beta-hairpin region. In contrast to the Z-DNA-contacting surface, the nonbinding surface of members of the Zalpha family are unrelated; this surface may effect protein-specific interactions. The presence of the conserved and tailored Z-DNA-binding surface, which interacts specifically with the zigzag backbone and syn base diagnostic of the Z-form, reinforces the importance to poxvirus infection of the ability of this protein to recognize the Z-conformation. | ||
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| - | A poxvirus protein forms a complex with left-handed Z-DNA: crystal structure of a Yatapoxvirus Zalpha bound to DNA.,Ha SC, Lokanath NK, Van Quyen D, Wu CA, Lowenhaupt K, Rich A, Kim YG, Kim KK Proc Natl Acad Sci U S A. 2004 Oct 5;101(40):14367-72. Epub 2004 Sep 24. PMID:15448208<ref>PMID:15448208</ref> | ||
| - | |||
| - | From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br> | ||
| - | </div> | ||
| - | <div class="pdbe-citations 1sfu" style="background-color:#fffaf0;"></div> | ||
==See Also== | ==See Also== | ||
*[[Z-DNA|Z-DNA]] | *[[Z-DNA|Z-DNA]] | ||
| - | == References == | ||
| - | <references/> | ||
__TOC__ | __TOC__ | ||
</StructureSection> | </StructureSection> | ||
| - | [[Category: | + | [[Category: Large Structures]] |
| - | [[Category: | + | [[Category: Yaba-like disease virus]] |
| - | [[Category: | + | [[Category: Ha SC]] |
| - | [[Category: Kim | + | [[Category: Kim KK]] |
| - | [[Category: | + | [[Category: Kim YG]] |
| - | [[Category: | + | [[Category: Lowenhaupt K]] |
| - | [[Category: Rich | + | [[Category: Rich A]] |
| - | [[Category: | + | [[Category: Van Quyen D]] |
| - | [[Category: | + | [[Category: Wu CA]] |
| - | + | ||
Current revision
Crystal structure of the viral Zalpha domain bound to left-handed Z-DNA
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Categories: Large Structures | Yaba-like disease virus | Ha SC | Kim KK | Kim YG | Lowenhaupt K | Rich A | Van Quyen D | Wu CA
