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| - | [[Image:2fgl.gif|left|200px]] | |
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| - | {{Structure
| + | ==An alkali thermostable F/10 xylanase from alkalophilic Bacillus sp. NG-27== |
| - | |PDB= 2fgl |SIZE=350|CAPTION= <scene name='initialview01'>2fgl</scene>, resolution 2.20Å
| + | <StructureSection load='2fgl' size='340' side='right'caption='[[2fgl]], [[Resolution|resolution]] 2.20Å' scene=''> |
| - | |SITE=
| + | == Structural highlights == |
| - | |LIGAND= <scene name='pdbligand=LXC:BETA-L-XYLOPYRANOSE'>LXC</scene>, <scene name='pdbligand=MG:MAGNESIUM+ION'>MG</scene>, <scene name='pdbligand=XYS:XYLOPYRANOSE'>XYS</scene> | + | <table><tr><td colspan='2'>[[2fgl]] is a 2 chain structure with sequence from [https://en.wikipedia.org/wiki/Bacillus_sp._NG-27 Bacillus sp. NG-27]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2FGL OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=2FGL FirstGlance]. <br> |
| - | |ACTIVITY= <span class='plainlinks'>[http://en.wikipedia.org/wiki/Endo-1,4-beta-xylanase Endo-1,4-beta-xylanase], with EC number [http://www.brenda-enzymes.info/php/result_flat.php4?ecno=3.2.1.8 3.2.1.8] </span>
| + | </td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution|Resolution]] 2.2Å</td></tr> |
| - | |GENE=
| + | <tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=LXC:BETA-L-XYLOPYRANOSE'>LXC</scene>, <scene name='pdbligand=MG:MAGNESIUM+ION'>MG</scene>, <scene name='pdbligand=XYS:XYLOPYRANOSE'>XYS</scene></td></tr> |
| - | |DOMAIN=
| + | <tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=2fgl FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=2fgl OCA], [https://pdbe.org/2fgl PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=2fgl RCSB], [https://www.ebi.ac.uk/pdbsum/2fgl PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=2fgl ProSAT]</span></td></tr> |
| - | |RELATEDENTRY=[[2f8q|2F8Q]]
| + | </table> |
| - | |RESOURCES=<span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=2fgl FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=2fgl OCA], [http://www.ebi.ac.uk/pdbsum/2fgl PDBsum], [http://www.rcsb.org/pdb/explore.do?structureId=2fgl RCSB]</span>
| + | == Function == |
| - | }}
| + | [https://www.uniprot.org/uniprot/O30700_9BACI O30700_9BACI] |
| - | | + | == Evolutionary Conservation == |
| - | '''An alkali thermostable F/10 xylanase from alkalophilic Bacillus sp. NG-27'''
| + | [[Image:Consurf_key_small.gif|200px|right]] |
| - | | + | Check<jmol> |
| - | | + | <jmolCheckbox> |
| - | ==Overview== | + | <scriptWhenChecked>; select protein; define ~consurf_to_do selected; consurf_initial_scene = true; script "/wiki/ConSurf/fg/2fgl_consurf.spt"</scriptWhenChecked> |
| - | Crystal structures are known for several glycosyl hydrolase family 10 (GH10) xylanases. However, none of them is from an alkalophilic organism that can grow in alkaline conditions. We have determined the crystal structures at 2.2 Angstroms of a GH10 extracellular endoxylanase (BSX) from an alkalophilic Bacillus sp. NG-27, for the native and the complex enzyme with xylosaccharides. The industrially important enzyme is optimally active and stable at 343 K and at a pH of 8.4. Comparison of the structure of BSX with those of other thermostable GH10 xylanases optimally active at acidic or close to neutral pH showed that the solvent-exposed acidic amino acids, Asp and Glu, are markedly enhanced in BSX, while solvent-exposed Asn was noticeably depleted. The BSX crystal structure when compared with putative three-dimensional homology models of other extracellular alkalophilic GH10 xylanases from alkalophilic organisms suggests that a protein surface rich in acidic residues may be an important feature common to these alkali thermostable enzymes. A comparison of the surface features of BSX and of halophilic proteins allowed us to predict the activity of BSX at high salt concentrations, which we verified through experiments. This offered us important lessons in the polyextremophilicity of proteins, where understanding the structural features of a protein stable in one set of extreme conditions provided clues about the activity of the protein in other extreme conditions. The work brings to the fore the role of the nature and composition of solvent-exposed residues in the adaptation of enzymes to polyextreme conditions, as in BSX.
| + | <scriptWhenUnchecked>script /wiki/extensions/Proteopedia/spt/initialview01.spt</scriptWhenUnchecked> |
| - | | + | <text>to colour the structure by Evolutionary Conservation</text> |
| - | ==About this Structure== | + | </jmolCheckbox> |
| - | 2FGL is a [[Single protein]] structure of sequence from [http://en.wikipedia.org/wiki/Bacteria Bacteria]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2FGL OCA].
| + | </jmol>, as determined by [http://consurfdb.tau.ac.il/ ConSurfDB]. You may read the [[Conservation%2C_Evolutionary|explanation]] of the method and the full data available from [http://bental.tau.ac.il/new_ConSurfDB/main_output.php?pdb_ID=2fgl ConSurf]. |
| - | | + | <div style="clear:both"></div> |
| - | ==Reference==
| + | __TOC__ |
| - | Crystal structures of native and xylosaccharide-bound alkali thermostable xylanase from an alkalophilic Bacillus sp. NG-27: structural insights into alkalophilicity and implications for adaptation to polyextreme conditions., Manikandan K, Bhardwaj A, Gupta N, Lokanath NK, Ghosh A, Reddy VS, Ramakumar S, Protein Sci. 2006 Aug;15(8):1951-60. Epub 2006 Jul 5. PMID:[http://www.ncbi.nlm.nih.gov/pubmed/16823036 16823036]
| + | </StructureSection> |
| - | [[Category: Bacteria]]
| + | [[Category: Bacillus sp. NG-27]] |
| - | [[Category: Endo-1,4-beta-xylanase]] | + | [[Category: Large Structures]] |
| - | [[Category: Single protein]] | + | [[Category: Bhardwaj A]] |
| - | [[Category: Bhardwaj, A.]] | + | [[Category: Ghosh A]] |
| - | [[Category: Ghosh, A.]] | + | [[Category: Lokanath NK]] |
| - | [[Category: Lokanath, N K.]] | + | [[Category: Manikandan K]] |
| - | [[Category: Manikandan, K.]] | + | [[Category: Ramakumar S]] |
| - | [[Category: Ramakumar, S.]] | + | [[Category: Reddy VS]] |
| - | [[Category: Reddy, V S.]] | + | |
| - | [[Category: f/10 family alkali-thermostable xylanase]]
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| - | ''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Mon Mar 31 03:02:29 2008''
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