1kgq

<StructureSection load='1kgq' size='340' side='right' caption='[[1kgq]], [[Resolution|resolution]] 2.00&Aring;' scene=''>

<table><tr><td colspan='2'>[[1kgq]] is a 1 chain structure with sequence from [http://en.wikipedia.org/wiki/"mycobacterium_tuberculosis_typus_bovinus"_lehmann_and_neumann_1907 "mycobacterium tuberculosis typus bovinus" lehmann and neumann 1907]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1KGQ OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=1KGQ FirstGlance]. <br>

</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=NPI:(2S)-2-AMINOHEPTANEDIOIC+ACID'>NPI</scene>, <scene name='pdbligand=SCO:SUCCINAMIDE-COA'>SCO</scene></td></tr>

<tr id='related'><td class="sblockLbl"><b>[[Related_structure|Related:]]</b></td><td class="sblockDat">[[1tdt|1tdt]], [[2tdt|2tdt]], [[3tdt|3tdt]], [[1kgt|1kgt]]</td></tr>

<tr id='gene'><td class="sblockLbl"><b>[[Gene|Gene:]]</b></td><td class="sblockDat">dapD ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=1765 "Mycobacterium tuberculosis typus bovinus" Lehmann and Neumann 1907])</td></tr>

<tr id='activity'><td class="sblockLbl"><b>Activity:</b></td><td class="sblockDat"><span class='plainlinks'>[http://en.wikipedia.org/wiki/2,3,4,5-tetrahydropyridine-2,6-dicarboxylate_N-succinyltransferase 2,3,4,5-tetrahydropyridine-2,6-dicarboxylate N-succinyltransferase], with EC number [http://www.brenda-enzymes.info/php/result_flat.php4?ecno=2.3.1.117 2.3.1.117] </span></td></tr>

<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=1kgq FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1kgq OCA], [http://pdbe.org/1kgq PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=1kgq RCSB], [http://www.ebi.ac.uk/pdbsum/1kgq PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=1kgq ProSAT]</span></td></tr>

[[http://www.uniprot.org/uniprot/DAPD_MYCBO DAPD_MYCBO]] Catalyzes the conversion of the cyclic tetrahydrodipicolinate (THDP) into the acyclic N-succinyl-L-2-amino-6-oxopimelate using succinyl-CoA (By similarity).

<div style="background-color:#fffaf0;">

== Publication Abstract from PubMed ==

Tetrahydrodipicolinate N-succinyltransferase (DapD) catalyzes the succinyl-CoA-dependent acylation of L-2-amino-6-oxopimelate to 2-N-succinyl-6-oxopimelate as part of the succinylase branch of the meso-diaminopimelate/lysine biosynthetic pathway of bacteria, blue-green algae, and plants. This pathway provides meso-diaminopimelate as a building block for cell wall peptidoglycan in most bacteria, and is regarded as a target pathway for antibacterial agents. We have solved the X-ray crystal structures of DapD in ternary complexes with pimelate/succinyl-CoA and L-2-aminopimelate with the nonreactive cofactor analog, succinamide-CoA. These structures define the binding conformation of the cofactor succinyl group and its interactions with the enzyme and place its thioester carbonyl carbon in close proximity to the nucleophilic 2-amino group of the acceptor, in support of a direct attack ternary complex mechanism. The acyl group specificity differences between homologous tetrahydrodipicolinate N-acetyl- and N-succinyltransferases can be rationalized with reference to at least three amino acids that interact with or give accessible active site volume to the cofactor succinyl group. These residues account at least in part for the substrate specificity that commits metabolic intermediates to either the succinylase or acetylase branches of the meso-diaminopimelate/lysine biosynthetic pathway.

Acyl group specificity at the active site of tetrahydridipicolinate N-succinyltransferase.,Beaman TW, Vogel KW, Drueckhammer DG, Blanchard JS, Roderick SL Protein Sci. 2002 Apr;11(4):974-9. PMID:11910040<ref>PMID:11910040</ref>

From MEDLINE&reg;/PubMed&reg;, a database of the U.S. National Library of Medicine.<br>

</div>

<div class="pdbe-citations 1kgq" style="background-color:#fffaf0;"></div>

== References ==

<references/>

[[Category: Mycobacterium tuberculosis typus bovinus lehmann and neumann 1907]]

[[Category: 2,3,4,5-tetrahydropyridine-2,6-dicarboxylate N-succinyltransferase]]

[[Category: Beaman, T W]]

[[Category: Blanchard, J S]]

[[Category: Drueckhammer, D G]]

[[Category: Roderick, S L]]

[[Category: Vogel, K W]]

[[Category: Transferase]]