|
|
| (2 intermediate revisions not shown.) |
| Line 1: |
Line 1: |
| | | | |
| | ==Crystal structure of recombinant Erythrina corallodandron Lectin== | | ==Crystal structure of recombinant Erythrina corallodandron Lectin== |
| - | <StructureSection load='1sfy' size='340' side='right' caption='[[1sfy]], [[Resolution|resolution]] 2.55Å' scene=''> | + | <StructureSection load='1sfy' size='340' side='right'caption='[[1sfy]], [[Resolution|resolution]] 2.55Å' scene=''> |
| | == Structural highlights == | | == Structural highlights == |
| - | <table><tr><td colspan='2'>[[1sfy]] is a 6 chain structure with sequence from [http://en.wikipedia.org/wiki/Coral_tree Coral tree]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1SFY OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=1SFY FirstGlance]. <br> | + | <table><tr><td colspan='2'>[[1sfy]] is a 6 chain structure with sequence from [https://en.wikipedia.org/wiki/Erythrina_corallodendron Erythrina corallodendron]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1SFY OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=1SFY FirstGlance]. <br> |
| - | </td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=CA:CALCIUM+ION'>CA</scene>, <scene name='pdbligand=LAT:BETA-LACTOSE'>LAT</scene>, <scene name='pdbligand=MN:MANGANESE+(II)+ION'>MN</scene></td></tr> | + | </td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution|Resolution]] 2.55Å</td></tr> |
| - | <tr id='related'><td class="sblockLbl"><b>[[Related_structure|Related:]]</b></td><td class="sblockDat">[[1ax0|1ax0]], [[1fyu|1fyu]]</td></tr>
| + | <tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=BGC:BETA-D-GLUCOSE'>BGC</scene>, <scene name='pdbligand=CA:CALCIUM+ION'>CA</scene>, <scene name='pdbligand=GAL:BETA-D-GALACTOSE'>GAL</scene>, <scene name='pdbligand=MN:MANGANESE+(II)+ION'>MN</scene>, <scene name='pdbligand=PRD_900004:beta-lactose'>PRD_900004</scene></td></tr> |
| - | <tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=1sfy FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1sfy OCA], [http://pdbe.org/1sfy PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=1sfy RCSB], [http://www.ebi.ac.uk/pdbsum/1sfy PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=1sfy ProSAT]</span></td></tr> | + | <tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=1sfy FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1sfy OCA], [https://pdbe.org/1sfy PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=1sfy RCSB], [https://www.ebi.ac.uk/pdbsum/1sfy PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=1sfy ProSAT]</span></td></tr> |
| | </table> | | </table> |
| | == Function == | | == Function == |
| - | [[http://www.uniprot.org/uniprot/LEC_ERYCO LEC_ERYCO]] Galactose and N-acetyllactosamine specific lectin. Binds to the H-2 blood type determinant fucosyl-N-acetyllactosamine. | + | [https://www.uniprot.org/uniprot/LEC_ERYCO LEC_ERYCO] Galactose and N-acetyllactosamine specific lectin. Binds to the H-2 blood type determinant fucosyl-N-acetyllactosamine. |
| | == Evolutionary Conservation == | | == Evolutionary Conservation == |
| | [[Image:Consurf_key_small.gif|200px|right]] | | [[Image:Consurf_key_small.gif|200px|right]] |
| Line 33: |
Line 33: |
| | __TOC__ | | __TOC__ |
| | </StructureSection> | | </StructureSection> |
| - | [[Category: Coral tree]] | + | [[Category: Erythrina corallodendron]] |
| - | [[Category: Kulkarni, K A]] | + | [[Category: Large Structures]] |
| - | [[Category: Mitra, N]] | + | [[Category: Kulkarni KA]] |
| - | [[Category: Srivastava, A]] | + | [[Category: Mitra N]] |
| - | [[Category: Suguna, K]] | + | [[Category: Srivastava A]] |
| - | [[Category: Surolia, A]] | + | [[Category: Suguna K]] |
| - | [[Category: Vijayan, M]] | + | [[Category: Surolia A]] |
| - | [[Category: Erythrina lectin]]
| + | [[Category: Vijayan M]] |
| - | [[Category: Glycosylation]]
| + | |
| - | [[Category: Legume lectin]]
| + | |
| - | [[Category: Sugar binding protein]]
| + | |
| Structural highlights
1sfy is a 6 chain structure with sequence from Erythrina corallodendron. Full crystallographic information is available from OCA. For a guided tour on the structure components use FirstGlance.
| | Method: | X-ray diffraction, Resolution 2.55Å |
| Ligands: | , , , , |
| Resources: | FirstGlance, OCA, PDBe, RCSB, PDBsum, ProSAT |
Function
LEC_ERYCO Galactose and N-acetyllactosamine specific lectin. Binds to the H-2 blood type determinant fucosyl-N-acetyllactosamine.
Evolutionary Conservation
Check, as determined by ConSurfDB. You may read the explanation of the method and the full data available from ConSurf.
Publication Abstract from PubMed
The three-dimensional structure of the recombinant form of Erythrina corallodendron lectin, complexed with lactose, has been elucidated by X-ray crystallography at 2.55 A resolution. Comparison of this non-glycosylated structure with that of the native glycosylated lectin reveals that the tertiary and quaternary structures are identical in the two forms, with local changes observed at one of the glycosylation sites (Asn17). These changes take place in such a way that hydrogen bonds with the neighboring protein molecules in rECorL compensate those made by the glycan with the protein in ECorL. Contrary to an earlier report, this study demonstrates that the glycan attached to the lectin does not influence the oligomeric state of the lectin. Identical interactions between the lectin and the non-covalently bound lactose in the two forms indicate, in line with earlier reports, that glycosylation does not affect the carbohydrate specificity of the lectin. The present study, the first of its kind involving a glycosylated protein with a well-defined glycan and the corresponding deglycosylated form, provides insights into the structural aspects of protein glycosylation.
Effect of glycosylation on the structure of Erythrina corallodendron lectin.,Kulkarni KA, Srivastava A, Mitra N, Sharon N, Surolia A, Vijayan M, Suguna K Proteins. 2004 Sep 1;56(4):821-7. PMID:15281133[1]
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.
References
- ↑ Kulkarni KA, Srivastava A, Mitra N, Sharon N, Surolia A, Vijayan M, Suguna K. Effect of glycosylation on the structure of Erythrina corallodendron lectin. Proteins. 2004 Sep 1;56(4):821-7. PMID:15281133 doi:10.1002/prot.20168
|
