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| | ==SOLUTION STRUCTURE OF RP 71955, A NEW 21 AMINO ACID TRICYCLIC PEPTIDE ACTIVE AGAINST HIV-1 VIRUS== | | ==SOLUTION STRUCTURE OF RP 71955, A NEW 21 AMINO ACID TRICYCLIC PEPTIDE ACTIVE AGAINST HIV-1 VIRUS== |
| - | <StructureSection load='1rpc' size='340' side='right' caption='[[1rpc]], [[NMR_Ensembles_of_Models | 20 NMR models]]' scene=''> | + | <StructureSection load='1rpc' size='340' side='right'caption='[[1rpc]]' scene=''> |
| | == Structural highlights == | | == Structural highlights == |
| - | <table><tr><td colspan='2'>[[1rpc]] is a 1 chain structure with sequence from [http://en.wikipedia.org/wiki/Actinomycetes,_strain_sp9440 Actinomycetes, strain sp9440]. Full experimental information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1RPC OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=1RPC FirstGlance]. <br> | + | <table><tr><td colspan='2'>[[1rpc]] is a 1 chain structure with sequence from [https://en.wikipedia.org/wiki/Actinomycete_Sp9440 Actinomycete Sp9440]. Full experimental information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1RPC OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=1RPC FirstGlance]. <br> |
| - | </td></tr><tr id='related'><td class="sblockLbl"><b>[[Related_structure|Related:]]</b></td><td class="sblockDat">[[1rpb|1rpb]]</td></tr> | + | </td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">Solution NMR, 20 models</td></tr> |
| - | <tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=1rpc FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1rpc OCA], [http://pdbe.org/1rpc PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=1rpc RCSB], [http://www.ebi.ac.uk/pdbsum/1rpc PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=1rpc ProSAT]</span></td></tr> | + | <tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=1rpc FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1rpc OCA], [https://pdbe.org/1rpc PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=1rpc RCSB], [https://www.ebi.ac.uk/pdbsum/1rpc PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=1rpc ProSAT]</span></td></tr> |
| | </table> | | </table> |
| | + | == Function == |
| | + | [https://www.uniprot.org/uniprot/3CP1_STRS9 3CP1_STRS9] |
| | <div style="background-color:#fffaf0;"> | | <div style="background-color:#fffaf0;"> |
| | == Publication Abstract from PubMed == | | == Publication Abstract from PubMed == |
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| | __TOC__ | | __TOC__ |
| | </StructureSection> | | </StructureSection> |
| - | [[Category: Actinomycetes, strain sp9440]] | + | [[Category: Actinomycete Sp9440]] |
| - | [[Category: Faucher, D]] | + | [[Category: Large Structures]] |
| - | [[Category: Frechet, D]] | + | [[Category: Faucher D]] |
| - | [[Category: Guitton, J D]] | + | [[Category: Frechet D]] |
| - | [[Category: Helynck, G]] | + | [[Category: Guitton JD]] |
| - | [[Category: Herman, F]] | + | [[Category: Helynck G]] |
| - | [[Category: James-Surcouf, E]] | + | [[Category: Herman F]] |
| - | [[Category: Ridoux, J P]] | + | [[Category: James-Surcouf E]] |
| - | [[Category: Sorbier, B Monegier Du]] | + | [[Category: Monegier Du Sorbier B]] |
| - | [[Category: Vuilhorgne, M]] | + | [[Category: Ridoux JP]] |
| - | [[Category: Hiv replication inhibitor]]
| + | [[Category: Vuilhorgne M]] |
| - | [[Category: Replication inhibitor]]
| + | |
| Structural highlights
Function
3CP1_STRS9
Publication Abstract from PubMed
The structure of RP 71955, a new tricyclic 21 amino acid peptide active against human immunodeficiency virus 1, was determined. Its amino acid composition was inferred from the results of fast atom bombardment mass spectrometry, nuclear magnetic resonance, Raman spectroscopy, and amino acid analysis. Its sequence could not be determined classically, using Edman degradation, given the lack of a free terminal NH2. It was deduced from the interpretation of interresidue nuclear Overhauser effects and confirmed by the sequencing of peptides obtained by limited chemical hydrolysis. It was found to be CLGIGSCNDFAGCGYAVVCFW. An internal amide bond between the NH2 of C1 and the gamma-COOH of D9 was observed, as well as two disulfide bridges, one between C1 and C13 and one between C7 and C19. The three-dimensional structure of RP 71955 was determined from nuclear magnetic resonance derived constraints using distance geometry, restrained molecular dynamics, nuclear Overhauser effect back calculation, and an iterative refinement using a full relaxation matrix approach. Analogies between the structure of RP 71955 and some functional domains of gp41, the transmembrane protein of human immunodeficiency virus 1, suggest hypotheses concerning the mode of action of RP 71955.
Solution structure of RP 71955, a new 21 amino acid tricyclic peptide active against HIV-1 virus.,Frechet D, Guitton JD, Herman F, Faucher D, Helynck G, Monegier du Sorbier B, Ridoux JP, James-Surcouf E, Vuilhorgne M Biochemistry. 1994 Jan 11;33(1):42-50. PMID:8286361[1]
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.
References
- ↑ Frechet D, Guitton JD, Herman F, Faucher D, Helynck G, Monegier du Sorbier B, Ridoux JP, James-Surcouf E, Vuilhorgne M. Solution structure of RP 71955, a new 21 amino acid tricyclic peptide active against HIV-1 virus. Biochemistry. 1994 Jan 11;33(1):42-50. PMID:8286361
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