2fip

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[[Image:2fip.gif|left|200px]]
 
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{{Structure
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==Phage phi29 transcription regulator p4==
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|PDB= 2fip |SIZE=350|CAPTION= <scene name='initialview01'>2fip</scene>, resolution 2.000&Aring;
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<StructureSection load='2fip' size='340' side='right'caption='[[2fip]], [[Resolution|resolution]] 2.00&Aring;' scene=''>
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|SITE=
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== Structural highlights ==
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|LIGAND=
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<table><tr><td colspan='2'>[[2fip]] is a 6 chain structure with sequence from [https://en.wikipedia.org/wiki/Bacillus_virus_phi29 Bacillus virus phi29]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2FIP OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=2FIP FirstGlance]. <br>
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|ACTIVITY=
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</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution|Resolution]] 2&#8491;</td></tr>
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|GENE= 4 ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=10756 Bacillus phage phi29])
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<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=2fip FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=2fip OCA], [https://pdbe.org/2fip PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=2fip RCSB], [https://www.ebi.ac.uk/pdbsum/2fip PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=2fip ProSAT]</span></td></tr>
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|DOMAIN=
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</table>
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|RELATEDENTRY=[[2fio|2FIO]]
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== Function ==
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|RESOURCES=<span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=2fip FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=2fip OCA], [http://www.ebi.ac.uk/pdbsum/2fip PDBsum], [http://www.rcsb.org/pdb/explore.do?structureId=2fip RCSB]</span>
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[https://www.uniprot.org/uniprot/TF4_BPPH2 TF4_BPPH2] Mediates, together with protein p6, the early to late transcriptional switch by stabilizing the binding of host RNA polymerase (RNAP) to the late A3 promoter. Activates transcription from the late A3 promoter and represses the main early promoters A2b and A2c by modifying the topology of the sequences encompassing early promoters A2c and A2b and late promoter A3 in a hairpin. Proteins p6 and p4 bind cooperatively to an approximately 200 bp DNA region located between the late A3 and the early A2c promoters. Binding of p4 molecules induces the reorganization of the binding of protein p6, giving rise to the nucleoprotein complex responsible for the switch from early to late transcription.<ref>PMID:11689446</ref> <ref>PMID:12426390</ref> <ref>PMID:14757050</ref> <ref>PMID:2107318</ref> <ref>PMID:21614197</ref> <ref>PMID:8617213</ref> <ref>PMID:9784366</ref>
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}}
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== References ==
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<references/>
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'''Phage phi29 transcription regulator p4'''
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__TOC__
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</StructureSection>
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[[Category: Bacillus virus phi29]]
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==Overview==
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[[Category: Large Structures]]
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Protein p4 affects the transcriptional switch that divides bacteriophage phi29 infection in early and late phases. The synthesis of DNA replication proteins and p4 takes place in the early phase, while structural, morphogenesis, and lysis proteins are synthesized in the late phase. Transcriptional switch by p4 is achieved by activating the late promoter A3 and repressing the early promoters A2b and A2c. The crystal structure of p4 alone and in complex with a 41 bp DNA, including the A3 promoter binding site, helps us to understand how the phage cycle is controlled. Protein p4 has a unique alpha/beta fold that includes a DNA recognition motif consisting of two N-terminal beta turn substructures, or N-hooks, located at the tips of an elongated protein homodimer. The two N-hooks enter the major groove of the double helix, establishing base-specific contacts. A high DNA curvature allows p4 N-hooks to reach two major groove areas three helical turns apart, like a bow and its string.
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[[Category: Badia D]]
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[[Category: Camacho A]]
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==About this Structure==
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[[Category: Coll M]]
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2FIP is a [[Single protein]] structure of sequence from [http://en.wikipedia.org/wiki/Bacillus_phage_phi29 Bacillus phage phi29]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2FIP OCA].
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[[Category: Escandon C]]
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[[Category: Perez-Lago L]]
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==Reference==
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[[Category: Salas M]]
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The structure of phage phi29 transcription regulator p4-DNA complex reveals an N-hook motif for DNA., Badia D, Camacho A, Perez-Lago L, Escandon C, Salas M, Coll M, Mol Cell. 2006 Apr 7;22(1):73-81. PMID:[http://www.ncbi.nlm.nih.gov/pubmed/16600871 16600871]
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[[Category: Bacillus phage phi29]]
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[[Category: Single protein]]
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[[Category: Badia, D.]]
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[[Category: Camacho, A.]]
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[[Category: Coll, M.]]
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[[Category: Escandon, C.]]
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[[Category: Perez-Lago, L.]]
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[[Category: Salas, M.]]
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[[Category: n-hook dna-binding motif]]
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[[Category: protein-dna complex]]
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[[Category: transcription regulation]]
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''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Mon Mar 31 03:03:20 2008''
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Current revision

Phage phi29 transcription regulator p4

PDB ID 2fip

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