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2fl1

From Proteopedia

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[[Image:2fl1.jpg|left|200px]]
 
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{{Structure
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==Crystal structure of red fluorescent protein from Zoanthus, zRFP574, at 2.4A resolution==
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|PDB= 2fl1 |SIZE=350|CAPTION= <scene name='initialview01'>2fl1</scene>, resolution 2.4&Aring;
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<StructureSection load='2fl1' size='340' side='right'caption='[[2fl1]], [[Resolution|resolution]] 2.40&Aring;' scene=''>
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|SITE=
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== Structural highlights ==
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|LIGAND= <scene name='pdbligand=SO4:SULFATE+ION'>SO4</scene>, <scene name='pdbligand=XYG:[(4Z)-2-[(1Z)-ETHANIMIDOYL]-4-(4-HYDROXYBENZYLIDENE)-5-OXO-4,5-DIHYDRO-1H-IMIDAZOL-1-YL]ACETIC+ACID'>XYG</scene>
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<table><tr><td colspan='2'>[[2fl1]] is a 4 chain structure with sequence from [https://en.wikipedia.org/wiki/Zoanthus_sp. Zoanthus sp.]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2FL1 OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=2FL1 FirstGlance]. <br>
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|ACTIVITY=
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</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution|Resolution]] 2.4&#8491;</td></tr>
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|GENE=
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<tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=SO4:SULFATE+ION'>SO4</scene>, <scene name='pdbligand=XYG:[(4Z)-2-[(1Z)-ETHANIMIDOYL]-4-(4-HYDROXYBENZYLIDENE)-5-OXO-4,5-DIHYDRO-1H-IMIDAZOL-1-YL]ACETIC+ACID'>XYG</scene></td></tr>
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|DOMAIN=
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<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=2fl1 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=2fl1 OCA], [https://pdbe.org/2fl1 PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=2fl1 RCSB], [https://www.ebi.ac.uk/pdbsum/2fl1 PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=2fl1 ProSAT]</span></td></tr>
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|RELATEDENTRY=[[1xa9|1XA9]]
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</table>
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|RESOURCES=<span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=2fl1 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=2fl1 OCA], [http://www.ebi.ac.uk/pdbsum/2fl1 PDBsum], [http://www.rcsb.org/pdb/explore.do?structureId=2fl1 RCSB]</span>
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== Function ==
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}}
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[https://www.uniprot.org/uniprot/Q8T4U4_9CNID Q8T4U4_9CNID]
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== Evolutionary Conservation ==
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'''Crystal structure of red fluorescent protein from Zoanthus, zRFP574, at 2.4A resolution'''
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[[Image:Consurf_key_small.gif|200px|right]]
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Check<jmol>
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<jmolCheckbox>
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==Overview==
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<scriptWhenChecked>; select protein; define ~consurf_to_do selected; consurf_initial_scene = true; script "/wiki/ConSurf/fl/2fl1_consurf.spt"</scriptWhenChecked>
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<scriptWhenUnchecked>script /wiki/extensions/Proteopedia/spt/initialview01.spt</scriptWhenUnchecked>
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<text>to colour the structure by Evolutionary Conservation</text>
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</jmolCheckbox>
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</jmol>, as determined by [http://consurfdb.tau.ac.il/ ConSurfDB]. You may read the [[Conservation%2C_Evolutionary|explanation]] of the method and the full data available from [http://bental.tau.ac.il/new_ConSurfDB/main_output.php?pdb_ID=2fl1 ConSurf].
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<div style="clear:both"></div>
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<div style="background-color:#fffaf0;">
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== Publication Abstract from PubMed ==
The three-dimensional structure of the red fluorescent protein (RFP) zRFP574 from the button polyp Zoanthus sp. (two dimers per asymmetric unit, 231 x 4 amino acids) has been determined at 2.4 A resolution in space group C222(1). The crystal structure, refined to a crystallographic R factor of 0.203 (R(free) = 0.249), adopts the beta-barrel fold composed of 11 strands similar to that of the yellow fluorescent protein zYFP538. The zRFP574 chromophore, originating from the protein sequence Asp66-Tyr67-Gly68, has a two-ring structure typical of GFP-like proteins. The bond geometry of residue 66 shows the strong tendency of the corresponding C(alpha) atom to sp(2) hybridization as a consequence of N-acylimine bond formation. The zRFP574 chromophore contains the 65-66 cis-peptide bond characteristic of red fluorescent proteins. The chromophore phenolic ring adopts a cis conformation coplanar with the imidazolinone ring. The crystallographic study has revealed an unexpected chemical feature of the internal chromophore. A decarboxylated side chain of the chromophore-forming residue Asp66 has been observed in the structure. This additional post-translational modification is likely to play a key role in the bathochromic shift of the zRFP574 spectrum.
The three-dimensional structure of the red fluorescent protein (RFP) zRFP574 from the button polyp Zoanthus sp. (two dimers per asymmetric unit, 231 x 4 amino acids) has been determined at 2.4 A resolution in space group C222(1). The crystal structure, refined to a crystallographic R factor of 0.203 (R(free) = 0.249), adopts the beta-barrel fold composed of 11 strands similar to that of the yellow fluorescent protein zYFP538. The zRFP574 chromophore, originating from the protein sequence Asp66-Tyr67-Gly68, has a two-ring structure typical of GFP-like proteins. The bond geometry of residue 66 shows the strong tendency of the corresponding C(alpha) atom to sp(2) hybridization as a consequence of N-acylimine bond formation. The zRFP574 chromophore contains the 65-66 cis-peptide bond characteristic of red fluorescent proteins. The chromophore phenolic ring adopts a cis conformation coplanar with the imidazolinone ring. The crystallographic study has revealed an unexpected chemical feature of the internal chromophore. A decarboxylated side chain of the chromophore-forming residue Asp66 has been observed in the structure. This additional post-translational modification is likely to play a key role in the bathochromic shift of the zRFP574 spectrum.
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==About this Structure==
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Structure of a red fluorescent protein from Zoanthus, zRFP574, reveals a novel chromophore.,Pletneva N, Pletnev S, Tikhonova T, Popov V, Martynov V, Pletnev V Acta Crystallogr D Biol Crystallogr. 2006 May;62(Pt 5):527-32. Epub 2006, Apr 19. PMID:16627946<ref>PMID:16627946</ref>
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2FL1 is a [[Single protein]] structure of sequence from [http://en.wikipedia.org/wiki/Zoanthus_sp. Zoanthus sp.]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2FL1 OCA].
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==Reference==
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From MEDLINE&reg;/PubMed&reg;, a database of the U.S. National Library of Medicine.<br>
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Structure of a red fluorescent protein from Zoanthus, zRFP574, reveals a novel chromophore., Pletneva N, Pletnev S, Tikhonova T, Popov V, Martynov V, Pletnev V, Acta Crystallogr D Biol Crystallogr. 2006 May;62(Pt 5):527-32. Epub 2006, Apr 19. PMID:[http://www.ncbi.nlm.nih.gov/pubmed/16627946 16627946]
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</div>
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[[Category: Single protein]]
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<div class="pdbe-citations 2fl1" style="background-color:#fffaf0;"></div>
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[[Category: Zoanthus sp.]]
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[[Category: Martynov, V.]]
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[[Category: Pletnev, S.]]
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[[Category: Pletnev, V.]]
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[[Category: Pletneva, N.]]
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[[Category: Popov, B.]]
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[[Category: Tikhonova, T.]]
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[[Category: beta barrel]]
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[[Category: beta-can fold]]
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[[Category: button polyp]]
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[[Category: chromophore]]
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[[Category: crystal structure]]
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[[Category: emission maximum 574nm]]
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[[Category: fluorescent marker]]
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[[Category: intersubunit interface]]
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[[Category: red fluorescent protein]]
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[[Category: tightly packed tetramer]]
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[[Category: zoanthus sp.]]
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[[Category: zrfp574]]
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''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Mon Mar 31 03:04:12 2008''
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==See Also==
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*[[Green Fluorescent Protein 3D structures|Green Fluorescent Protein 3D structures]]
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== References ==
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<references/>
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__TOC__
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</StructureSection>
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[[Category: Large Structures]]
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[[Category: Zoanthus sp]]
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[[Category: Martynov V]]
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[[Category: Pletnev S]]
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[[Category: Pletnev V]]
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[[Category: Pletneva N]]
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[[Category: Popov B]]
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[[Category: Tikhonova T]]

Current revision

Crystal structure of red fluorescent protein from Zoanthus, zRFP574, at 2.4A resolution

PDB ID 2fl1

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