2flh

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[[Image:2flh.gif|left|200px]]
 
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{{Structure
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==Crystal structure of cytokinin-specific binding protein from mung bean in complex with cytokinin==
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|PDB= 2flh |SIZE=350|CAPTION= <scene name='initialview01'>2flh</scene>, resolution 1.20&Aring;
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<StructureSection load='2flh' size='340' side='right'caption='[[2flh]], [[Resolution|resolution]] 1.20&Aring;' scene=''>
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|SITE=
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== Structural highlights ==
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|LIGAND= <scene name='pdbligand=NA:SODIUM+ION'>NA</scene>, <scene name='pdbligand=ZEA:(2Z)-2-METHYL-4-(9H-PURIN-6-YLAMINO)-2-BUTEN-1-OL'>ZEA</scene>
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<table><tr><td colspan='2'>[[2flh]] is a 4 chain structure with sequence from [https://en.wikipedia.org/wiki/Vigna_radiata Vigna radiata]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2FLH OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=2FLH FirstGlance]. <br>
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|ACTIVITY=
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</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution|Resolution]] 1.2&#8491;</td></tr>
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|GENE= vrcsbp ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=157791 Vigna radiata])
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<tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=NA:SODIUM+ION'>NA</scene>, <scene name='pdbligand=ZEA:(2E)-2-METHYL-4-(9H-PURIN-6-YLAMINO)BUT-2-EN-1-OL'>ZEA</scene></td></tr>
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|DOMAIN=
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<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=2flh FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=2flh OCA], [https://pdbe.org/2flh PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=2flh RCSB], [https://www.ebi.ac.uk/pdbsum/2flh PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=2flh ProSAT]</span></td></tr>
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|RELATEDENTRY=[[1icx|1ICX]], [[1ifv|1IFV]], [[1xdf|1XDF]], [[1bv1|1BV1]]
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</table>
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|RESOURCES=<span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=2flh FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=2flh OCA], [http://www.ebi.ac.uk/pdbsum/2flh PDBsum], [http://www.rcsb.org/pdb/explore.do?structureId=2flh RCSB]</span>
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== Function ==
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}}
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[https://www.uniprot.org/uniprot/PHBP_VIGRR PHBP_VIGRR] Binds the cytokinin trans-zeatin in vitro (PubMed:16998071, PubMed:18453695). Binds gibberellin A3 (GA3) in vitro (PubMed:25004979).<ref>PMID:16998071</ref> <ref>PMID:18453695</ref> <ref>PMID:25004979</ref>
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== Evolutionary Conservation ==
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'''Crystal structure of cytokinin-specific binding protein from mung bean in complex with cytokinin'''
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[[Image:Consurf_key_small.gif|200px|right]]
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Check<jmol>
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<jmolCheckbox>
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==Overview==
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<scriptWhenChecked>; select protein; define ~consurf_to_do selected; consurf_initial_scene = true; script "/wiki/ConSurf/fl/2flh_consurf.spt"</scriptWhenChecked>
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The cytosolic fraction of Vigna radiata contains a 17-kD protein that binds plant hormones from the cytokinin group, such as zeatin. Using recombinant protein and isothermal titration calorimetry as well as fluorescence measurements coupled with ligand displacement, we have reexamined the K(d) values and show them to range from approximately 10(-6) M (for 4PU30) to 10(-4) M (for zeatin) for 1:1 stoichiometry complexes. In addition, we have crystallized this cytokinin-specific binding protein (Vr CSBP) in complex with zeatin and refined the structure to 1.2 A resolution. Structurally, Vr CSBP is similar to plant pathogenesis-related class 10 (PR-10) proteins, despite low sequence identity (&lt;20%). This unusual fold conservation reinforces the notion that classic PR-10 proteins have evolved to bind small-molecule ligands. The fold consists of an antiparallel beta-sheet wrapped around a C-terminal alpha-helix, with two short alpha-helices closing a cavity formed within the protein core. In each of the four independent CSBP molecules, there is a zeatin ligand located deep in the cavity with conserved conformation and protein-ligand interactions. In three cases, an additional zeatin molecule is found in variable orientation but with excellent definition in electron density, which plugs the entrance to the binding pocket, sealing the inner molecule from contact with bulk solvent.
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<scriptWhenUnchecked>script /wiki/extensions/Proteopedia/spt/initialview01.spt</scriptWhenUnchecked>
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<text>to colour the structure by Evolutionary Conservation</text>
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==About this Structure==
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</jmolCheckbox>
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2FLH is a [[Single protein]] structure of sequence from [http://en.wikipedia.org/wiki/Vigna_radiata Vigna radiata]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2FLH OCA].
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</jmol>, as determined by [http://consurfdb.tau.ac.il/ ConSurfDB]. You may read the [[Conservation%2C_Evolutionary|explanation]] of the method and the full data available from [http://bental.tau.ac.il/new_ConSurfDB/main_output.php?pdb_ID=2flh ConSurf].
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<div style="clear:both"></div>
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==Reference==
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== References ==
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Crystal structure of Vigna radiata cytokinin-specific binding protein in complex with zeatin., Pasternak O, Bujacz GD, Fujimoto Y, Hashimoto Y, Jelen F, Otlewski J, Sikorski MM, Jaskolski M, Plant Cell. 2006 Oct;18(10):2622-34. Epub 2006 Sep 22. PMID:[http://www.ncbi.nlm.nih.gov/pubmed/16998071 16998071]
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<references/>
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[[Category: Single protein]]
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__TOC__
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</StructureSection>
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[[Category: Large Structures]]
[[Category: Vigna radiata]]
[[Category: Vigna radiata]]
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[[Category: Pasternak, O.]]
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[[Category: Bujacz GD]]
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[[Category: cytokinin]]
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[[Category: Jaskolski M]]
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[[Category: multiple-ligand binding]]
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[[Category: Pasternak O]]
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[[Category: pathogenesis-related protein]]
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[[Category: Sikorski MM]]
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[[Category: zeatin]]
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''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Mon Mar 31 03:04:27 2008''
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Current revision

Crystal structure of cytokinin-specific binding protein from mung bean in complex with cytokinin

PDB ID 2flh

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