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| | ==High Resolution Structure of Mutant CA09-PB2cap== | | ==High Resolution Structure of Mutant CA09-PB2cap== |
| - | <StructureSection load='5wop' size='340' side='right' caption='[[5wop]], [[Resolution|resolution]] 1.52Å' scene=''> | + | <StructureSection load='5wop' size='340' side='right'caption='[[5wop]], [[Resolution|resolution]] 1.52Å' scene=''> |
| | == Structural highlights == | | == Structural highlights == |
| - | <table><tr><td colspan='2'>[[5wop]] is a 2 chain structure with sequence from [http://en.wikipedia.org/wiki/9infa 9infa]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=5WOP OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=5WOP FirstGlance]. <br> | + | <table><tr><td colspan='2'>[[5wop]] is a 2 chain structure with sequence from [https://en.wikipedia.org/wiki/Influenza_A_virus Influenza A virus]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=5WOP OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=5WOP FirstGlance]. <br> |
| - | </td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=GOL:GLYCEROL'>GOL</scene></td></tr> | + | </td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution|Resolution]] 1.52Å</td></tr> |
| - | <tr id='related'><td class="sblockLbl"><b>[[Related_structure|Related:]]</b></td><td class="sblockDat">[[5eg7|5eg7]], [[4enf|4enf]]</td></tr>
| + | <tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=GOL:GLYCEROL'>GOL</scene></td></tr> |
| - | <tr id='gene'><td class="sblockLbl"><b>[[Gene|Gene:]]</b></td><td class="sblockDat">PB2 ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=11320 9INFA])</td></tr> | + | <tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=5wop FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=5wop OCA], [https://pdbe.org/5wop PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=5wop RCSB], [https://www.ebi.ac.uk/pdbsum/5wop PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=5wop ProSAT]</span></td></tr> |
| - | <tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=5wop FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=5wop OCA], [http://pdbe.org/5wop PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=5wop RCSB], [http://www.ebi.ac.uk/pdbsum/5wop PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=5wop ProSAT]</span></td></tr> | + | |
| | </table> | | </table> |
| | + | == Function == |
| | + | [https://www.uniprot.org/uniprot/F4MEU3_9INFA F4MEU3_9INFA] |
| | <div style="background-color:#fffaf0;"> | | <div style="background-color:#fffaf0;"> |
| | == Publication Abstract from PubMed == | | == Publication Abstract from PubMed == |
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| | __TOC__ | | __TOC__ |
| | </StructureSection> | | </StructureSection> |
| - | [[Category: Constantinides, A E]] | + | [[Category: Influenza A virus]] |
| - | [[Category: Gumpper, R H]] | + | [[Category: Large Structures]] |
| - | [[Category: Luo, M]] | + | [[Category: Constantinides AE]] |
| - | [[Category: Severin, C]] | + | [[Category: Gumpper RH]] |
| - | [[Category: Cap-binding domain]] | + | [[Category: Luo M]] |
| - | [[Category: Cap-snatching]] | + | [[Category: Severin C]] |
| - | [[Category: Influenza]]
| + | |
| - | [[Category: Polymerase basic protein 2]]
| + | |
| - | [[Category: Rna polymerase]]
| + | |
| - | [[Category: Structural genomic]]
| + | |
| - | [[Category: Transcription]]
| + | |
| Structural highlights
Function
F4MEU3_9INFA
Publication Abstract from PubMed
In the face of increasing drug resistance and the rapidly increasing necessity for practicality in clinical settings, drugs targeting different viral proteins are needed in order to control influenza A and B. A small molecule that tenaciously adheres to the PB2cap binding domain, part of the heterotrimeric RNA polymerase machinery of influenza A virus and influenza B virus, is a promising drug candidate. Understanding the anatomic behavior of PB2cap upon ligand binding will aid in the development of a more robust inhibitor. In this report, the anatomic behavior of the influenza A virus PB2cap domain is established by solving the crystal structure of native influenza A virus PB2cap at 1.52 A resolution. By comparing it with the ligand-bound structure, the dissociation and rotation of the ligand-binding domain in PB2cap from the C-terminal domain is identified. This domain movement is present in many PB2cap structures, suggesting its functional relevance for polymerase activity.
High-resolution structure of the Influenza A virus PB2cap binding domain illuminates the changes induced by ligand binding.,Constantinides A, Gumpper R, Severin C, Luo M Acta Crystallogr F Struct Biol Commun. 2018 Mar 1;74(Pt 3):122-127. doi:, 10.1107/S2053230X18000894. Epub 2018 Feb 26. PMID:29497014[1]
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.
References
- ↑ Constantinides A, Gumpper R, Severin C, Luo M. High-resolution structure of the Influenza A virus PB2cap binding domain illuminates the changes induced by ligand binding. Acta Crystallogr F Struct Biol Commun. 2018 Mar 1;74(Pt 3):122-127. doi:, 10.1107/S2053230X18000894. Epub 2018 Feb 26. PMID:29497014 doi:http://dx.doi.org/10.1107/S2053230X18000894
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