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| | ==CORE-STREPTAVIDIN MUTANT D128A AT PH 4.5== | | ==CORE-STREPTAVIDIN MUTANT D128A AT PH 4.5== |
| - | <StructureSection load='1sws' size='340' side='right' caption='[[1sws]], [[Resolution|resolution]] 2.00Å' scene=''> | + | <StructureSection load='1sws' size='340' side='right'caption='[[1sws]], [[Resolution|resolution]] 2.00Å' scene=''> |
| | == Structural highlights == | | == Structural highlights == |
| - | <table><tr><td colspan='2'>[[1sws]] is a 4 chain structure with sequence from [http://en.wikipedia.org/wiki/As_4.1583 As 4.1583]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1SWS OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=1SWS FirstGlance]. <br> | + | <table><tr><td colspan='2'>[[1sws]] is a 4 chain structure with sequence from [https://en.wikipedia.org/wiki/Streptomyces_avidinii Streptomyces avidinii]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1SWS OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=1SWS FirstGlance]. <br> |
| - | </td></tr><tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=1sws FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1sws OCA], [http://pdbe.org/1sws PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=1sws RCSB], [http://www.ebi.ac.uk/pdbsum/1sws PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=1sws ProSAT]</span></td></tr> | + | </td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution|Resolution]] 2Å</td></tr> |
| | + | <tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=1sws FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1sws OCA], [https://pdbe.org/1sws PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=1sws RCSB], [https://www.ebi.ac.uk/pdbsum/1sws PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=1sws ProSAT]</span></td></tr> |
| | </table> | | </table> |
| | == Function == | | == Function == |
| - | [[http://www.uniprot.org/uniprot/SAV_STRAV SAV_STRAV]] The biological function of streptavidin is not known. Forms a strong non-covalent specific complex with biotin (one molecule of biotin per subunit of streptavidin). | + | [https://www.uniprot.org/uniprot/SAV_STRAV SAV_STRAV] The biological function of streptavidin is not known. Forms a strong non-covalent specific complex with biotin (one molecule of biotin per subunit of streptavidin). |
| | == Evolutionary Conservation == | | == Evolutionary Conservation == |
| | [[Image:Consurf_key_small.gif|200px|right]] | | [[Image:Consurf_key_small.gif|200px|right]] |
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| | ==See Also== | | ==See Also== |
| - | *[[Avidin|Avidin]] | + | *[[Avidin 3D structures|Avidin 3D structures]] |
| | == References == | | == References == |
| | <references/> | | <references/> |
| | __TOC__ | | __TOC__ |
| | </StructureSection> | | </StructureSection> |
| - | [[Category: As 4 1583]] | + | [[Category: Large Structures]] |
| - | [[Category: Chu, V]] | + | [[Category: Streptomyces avidinii]] |
| - | [[Category: Freitag, S]] | + | [[Category: Chu V]] |
| - | [[Category: Klumb, L A]] | + | [[Category: Freitag S]] |
| - | [[Category: Stayton, P S]]
| + | [[Category: Klumb LA]] |
| - | [[Category: Stenkamp, R E]] | + | [[Category: Le Trong I]] |
| - | [[Category: To, R]] | + | [[Category: Stayton PS]] |
| - | [[Category: Trong, I Le]] | + | [[Category: Stenkamp RE]] |
| - | [[Category: Binding protein]] | + | [[Category: To R]] |
| - | [[Category: Biotin binding protein]] | + | |
| Structural highlights
Function
SAV_STRAV The biological function of streptavidin is not known. Forms a strong non-covalent specific complex with biotin (one molecule of biotin per subunit of streptavidin).
Evolutionary Conservation
Check, as determined by ConSurfDB. You may read the explanation of the method and the full data available from ConSurf.
Publication Abstract from PubMed
It is currently unclear whether small molecules dissociate from a protein binding site along a defined pathway or through a collection of dissociation pathways. We report herein a joint crystallographic, computational, and biophysical study that suggests the Asp-128 --> Ala (D128A) streptavidin mutant closely mimics an intermediate on a well-defined dissociation pathway. Asp-128 is hydrogen bonded to a ureido nitrogen of biotin and also networks with the important aromatic binding contacts Trp-92 and Trp-108. The Asn-23 hydrogen bond to the ureido oxygen of biotin is lengthened to 3.8 A in the D128A structure, and a water molecule has moved into the pocket to replace the missing carboxylate interaction. These alterations are accompanied by the coupled movement of biotin, the flexible binding loop containing Ser-45, and the loop containing the Ser-27 hydrogen bonding contact. This structure closely parallels a key intermediate observed in a potential of mean force-simulated dissociation pathway of native streptavidin, where the Asn-23 hydrogen bond breaks first, accompanied by the replacement of the Asp-128 hydrogen bond by an entering water molecule. Furthermore, both biotin and the flexible loop move in a concerted conformational change that closely approximates the D128A structural changes. The activation and thermodynamic parameters for the D128A mutant were measured and are consistent with an intermediate that has traversed the early portion of the dissociation reaction coordinate through endothermic bond breaking and concomitant gain in configurational entropy. These composite results suggest that the D128A mutant provides a structural "snapshot" of an early intermediate on a relatively well-defined dissociation pathway for biotin.
A structural snapshot of an intermediate on the streptavidin-biotin dissociation pathway.,Freitag S, Chu V, Penzotti JE, Klumb LA, To R, Hyre D, Le Trong I, Lybrand TP, Stenkamp RE, Stayton PS Proc Natl Acad Sci U S A. 1999 Jul 20;96(15):8384-9. PMID:10411884[1]
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.
See Also
References
- ↑ Freitag S, Chu V, Penzotti JE, Klumb LA, To R, Hyre D, Le Trong I, Lybrand TP, Stenkamp RE, Stayton PS. A structural snapshot of an intermediate on the streptavidin-biotin dissociation pathway. Proc Natl Acad Sci U S A. 1999 Jul 20;96(15):8384-9. PMID:10411884
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