2frh

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[[Image:2frh.jpg|left|200px]]
 
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{{Structure
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==Crystal Structure of Sara, A Transcription Regulator From Staphylococcus Aureus==
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|PDB= 2frh |SIZE=350|CAPTION= <scene name='initialview01'>2frh</scene>, resolution 2.50&Aring;
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<StructureSection load='2frh' size='340' side='right'caption='[[2frh]], [[Resolution|resolution]] 2.50&Aring;' scene=''>
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|SITE=
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== Structural highlights ==
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|LIGAND= <scene name='pdbligand=CA:CALCIUM+ION'>CA</scene>
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<table><tr><td colspan='2'>[[2frh]] is a 2 chain structure with sequence from [https://en.wikipedia.org/wiki/Staphylococcus_aureus Staphylococcus aureus]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2FRH OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=2FRH FirstGlance]. <br>
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|ACTIVITY=
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</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution|Resolution]] 2.5&#8491;</td></tr>
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|GENE= sarA ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=1280 Staphylococcus aureus])
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<tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=CA:CALCIUM+ION'>CA</scene></td></tr>
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|DOMAIN=
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<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=2frh FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=2frh OCA], [https://pdbe.org/2frh PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=2frh RCSB], [https://www.ebi.ac.uk/pdbsum/2frh PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=2frh ProSAT]</span></td></tr>
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|RELATEDENTRY=[[2fnp|2FNP]]
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</table>
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|RESOURCES=<span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=2frh FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=2frh OCA], [http://www.ebi.ac.uk/pdbsum/2frh PDBsum], [http://www.rcsb.org/pdb/explore.do?structureId=2frh RCSB]</span>
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== Function ==
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}}
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[https://www.uniprot.org/uniprot/SARA_STAAW SARA_STAAW] Global regulator with both positive and negative effects that controls the expression of several virulence factors and the biofilm formation process in a cell density-dependent manner (By similarity). Required for transcription of primary transcripts RNAII and RNAIII generated by agr (virulence accessory gene regulator) locus. Negatively regulates the expression of spa (protein A) and aur (metalloprotease aureolysin).
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== Evolutionary Conservation ==
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'''Crystal Structure of Sara, A Transcription Regulator From Staphylococcus Aureus'''
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[[Image:Consurf_key_small.gif|200px|right]]
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Check<jmol>
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<jmolCheckbox>
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==Overview==
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<scriptWhenChecked>; select protein; define ~consurf_to_do selected; consurf_initial_scene = true; script "/wiki/ConSurf/fr/2frh_consurf.spt"</scriptWhenChecked>
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The sarA locus in Staphylococcus aureus controls the expression of many virulence genes. The sarA regulatory molecule, SarA, is a 14.7-kDa protein (124 residues) that binds to the promoter region of target genes. Here we report the 2.6 A-resolution x-ray crystal structure of the dimeric winged helix SarA protein, which differs from the published SarA structure dramatically. In the crystal packing, multiple dimers of SarA form a scaffold, possibly via divalent cations. Mutations of individual residues within the DNA-binding helix-turn-helix and the winged region as well as within the metal-binding pocket implicate basic residues R84 and R90 within the winged region to be critical in DNA binding, whereas acidic residues D88 and E89 (wing), D8 and E11 (metal-binding pocket), and cysteine 9 are essential for SarA function. These data suggest that the winged region of the winged helix protein participates in DNA binding and activation, whereas the putative divalent cation binding pocket is only involved in gene function.
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<scriptWhenUnchecked>script /wiki/extensions/Proteopedia/spt/initialview01.spt</scriptWhenUnchecked>
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<text>to colour the structure by Evolutionary Conservation</text>
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==About this Structure==
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</jmolCheckbox>
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2FRH is a [[Single protein]] structure of sequence from [http://en.wikipedia.org/wiki/Staphylococcus_aureus Staphylococcus aureus]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2FRH OCA].
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</jmol>, as determined by [http://consurfdb.tau.ac.il/ ConSurfDB]. You may read the [[Conservation%2C_Evolutionary|explanation]] of the method and the full data available from [http://bental.tau.ac.il/new_ConSurfDB/main_output.php?pdb_ID=2frh ConSurf].
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<div style="clear:both"></div>
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==Reference==
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__TOC__
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Structural and function analyses of the global regulatory protein SarA from Staphylococcus aureus., Liu Y, Manna AC, Pan CH, Kriksunov IA, Thiel DJ, Cheung AL, Zhang G, Proc Natl Acad Sci U S A. 2006 Feb 14;103(7):2392-7. Epub 2006 Feb 2. PMID:[http://www.ncbi.nlm.nih.gov/pubmed/16455801 16455801]
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</StructureSection>
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[[Category: Single protein]]
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[[Category: Large Structures]]
[[Category: Staphylococcus aureus]]
[[Category: Staphylococcus aureus]]
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[[Category: Cheung, A L.]]
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[[Category: Cheung AL]]
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[[Category: Ingavale, S.]]
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[[Category: Ingavale S]]
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[[Category: Liu, Y.]]
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[[Category: Liu Y]]
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[[Category: Manna, A C.]]
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[[Category: Manna AC]]
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[[Category: Zhang, G.]]
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[[Category: Zhang G]]
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[[Category: divalent metal binding]]
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[[Category: winged-helix protein]]
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''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Mon Mar 31 03:06:47 2008''
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Current revision

Crystal Structure of Sara, A Transcription Regulator From Staphylococcus Aureus

PDB ID 2frh

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