6cp6
From Proteopedia
(Difference between revisions)
(New page: '''Unreleased structure''' The entry 6cp6 is ON HOLD Authors: Srivastava, A.P., Luo, M., Symersky, J., Liao, M.F., Mueller, D.M. Description: Monomer yeast ATP synthase (F1Fo) reconsti...) |
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| - | '''Unreleased structure''' | ||
| - | + | ==Monomer yeast ATP synthase (F1Fo) reconstituted in nanodisc.== | |
| + | <SX load='6cp6' size='340' side='right' viewer='molstar' caption='[[6cp6]], [[Resolution|resolution]] 3.60Å' scene=''> | ||
| + | == Structural highlights == | ||
| + | <table><tr><td colspan='2'>[[6cp6]] is a 21 chain structure with sequence from [https://en.wikipedia.org/wiki/Saccharomyces_cerevisiae_S288C Saccharomyces cerevisiae S288C]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=6CP6 OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=6CP6 FirstGlance]. <br> | ||
| + | </td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">Electron Microscopy, [[Resolution|Resolution]] 3.6Å</td></tr> | ||
| + | <tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=ADP:ADENOSINE-5-DIPHOSPHATE'>ADP</scene>, <scene name='pdbligand=ATP:ADENOSINE-5-TRIPHOSPHATE'>ATP</scene>, <scene name='pdbligand=FME:N-FORMYLMETHIONINE'>FME</scene></td></tr> | ||
| + | <tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=6cp6 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=6cp6 OCA], [https://pdbe.org/6cp6 PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=6cp6 RCSB], [https://www.ebi.ac.uk/pdbsum/6cp6 PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=6cp6 ProSAT]</span></td></tr> | ||
| + | </table> | ||
| + | == Function == | ||
| + | [https://www.uniprot.org/uniprot/ATPO_YEAST ATPO_YEAST] Mitochondrial membrane ATP synthase (F(1)F(0) ATP synthase or Complex V) produces ATP from ADP in the presence of a proton gradient across the membrane which is generated by electron transport complexes of the respiratory chain. F-type ATPases consist of two structural domains, F(1) - containing the extramembraneous catalytic core and F(0) - containing the membrane proton channel, linked together by a central stalk and a peripheral stalk. During catalysis, ATP synthesis in the catalytic domain of F(1) is coupled via a rotary mechanism of the central stalk subunits to proton translocation. Part of the complex F(0) domain and the peripheric stalk, which acts as a stator to hold the catalytic alpha(3)beta(3) subcomplex and subunit a/ATP6 static relative to the rotary elements. | ||
| + | <div style="background-color:#fffaf0;"> | ||
| + | == Publication Abstract from PubMed == | ||
| + | Mitochondrial ATP synthase comprises a membrane embedded Fo motor that rotates to drive ATP synthesis in the F1 subunit. We used single-particle cryo-EM to obtain structures of the full complex in a lipid bilayer in the absence or presence of the inhibitor oligomycin, at 3.6 A and 3.8 A resolution, respectively. To limit conformational heterogeneity, we locked the rotor in a single conformation by fusing the F6 subunit of the stator with the delta-subunit of the rotor. Assembly of the enzyme with the F6-delta fusion caused a twisting of the rotor and a 9 degrees rotation of the Fo c10-ring in the direction of ATP synthesis, relative to the structure of isolated Fo Our cryo-EM structures show how F1 and Fo are coupled, give insight into the proton translocation pathway and show how oligomycin blocks ATP synthesis. | ||
| - | + | High-resolution cryo-EM analysis of the yeast ATP synthase in a lipid membrane.,Srivastava AP, Luo M, Zhou W, Symersky J, Bai D, Chambers MG, Faraldo-Gomez JD, Liao M, Mueller DM Science. 2018 Apr 12. pii: science.aas9699. doi: 10.1126/science.aas9699. PMID:29650704<ref>PMID:29650704</ref> | |
| - | + | From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br> | |
| - | [[Category: | + | </div> |
| - | [[Category: | + | <div class="pdbe-citations 6cp6" style="background-color:#fffaf0;"></div> |
| - | [[Category: | + | |
| - | [[Category: Luo | + | ==See Also== |
| - | [[Category: | + | *[[ATPase 3D structures|ATPase 3D structures]] |
| - | [[Category: Srivastava | + | == References == |
| + | <references/> | ||
| + | __TOC__ | ||
| + | </SX> | ||
| + | [[Category: Large Structures]] | ||
| + | [[Category: Saccharomyces cerevisiae S288C]] | ||
| + | [[Category: Liao MF]] | ||
| + | [[Category: Luo M]] | ||
| + | [[Category: Mueller DM]] | ||
| + | [[Category: Srivastava AP]] | ||
| + | [[Category: Symersky J]] | ||
Current revision
Monomer yeast ATP synthase (F1Fo) reconstituted in nanodisc.
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