6fzn

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(New page: '''Unreleased structure''' The entry 6fzn is ON HOLD until Paper Publication Authors: Janowski, R., Fuenzalida-Wernera, J.P., Mishra, K., Vetschera, P., Weidenfeld, I., Richter, K., Nie...)
Current revision (05:14, 21 November 2024) (edit) (undo)
 
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'''Unreleased structure'''
 
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The entry 6fzn is ON HOLD until Paper Publication
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==SMURFP-Y56R mutant in complex with biliverdin==
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<StructureSection load='6fzn' size='340' side='right'caption='[[6fzn]], [[Resolution|resolution]] 2.50&Aring;' scene=''>
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== Structural highlights ==
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<table><tr><td colspan='2'>[[6fzn]] is a 2 chain structure with sequence from [https://en.wikipedia.org/wiki/Synthetic_construct Synthetic construct]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=6FZN OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=6FZN FirstGlance]. <br>
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</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution|Resolution]] 2.5&#8491;</td></tr>
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<tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=9UY:3-[5-[[(3~{R},4~{R})-3-ethenyl-4-methyl-5-oxidanylidene-3,4-dihydropyrrol-2-yl]methyl]-2-[[5-[(4-ethenyl-3-methyl-5-oxidanylidene-pyrrol-2-yl)methyl]-3-(3-hydroxy-3-oxopropyl)-4-methyl-1~{H}-pyrrol-2-yl]methyl]-4-methyl-1~{H}-pyrrol-3-yl]propanoic+acid'>9UY</scene></td></tr>
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<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=6fzn FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=6fzn OCA], [https://pdbe.org/6fzn PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=6fzn RCSB], [https://www.ebi.ac.uk/pdbsum/6fzn PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=6fzn ProSAT]</span></td></tr>
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</table>
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<div style="background-color:#fffaf0;">
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== Publication Abstract from PubMed ==
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Small, ultra-red fluorescence protein (smURFP) introduces the non-native biliverdin (BV) chromophore to phycobiliproteins (PBPs), allowing them to be used as transgenic labels for in vivo mammalian imaging. Presently, no structural information exists for PBPs bound to the non-native BV chromophore, which limits the further development of smURFP and related proteins as imaging labels or indicators. Here we describe the first crystal structure of a PBP bound to BV. The structures of smURFP-Y56R with BV and smURFP-Y56F without BV reveal unique oligomerization interfaces different from those in wild-type PBPs bound to native chromophores. Our structures suggest that the oligomerization interface affects the BV binding site, creating a link between oligomerization and chromophorylation that we confirmed through site-directed mutagenesis and that may help guide efforts to improve the notorious chromophorylation of smURFP and other PBPs engineered to bind BV.
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Authors: Janowski, R., Fuenzalida-Wernera, J.P., Mishra, K., Vetschera, P., Weidenfeld, I., Richter, K., Niessing, D., Ntziachristos, V., Stiel, A.C.
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Crystal structure of a biliverdin-bound phycobiliprotein: Interdependence of oligomerization and chromophorylation.,Fuenzalida-Werner JP, Janowski R, Mishra K, Weidenfeld I, Niessing D, Ntziachristos V, Stiel AC J Struct Biol. 2018 Oct 1. pii: S1047-8477(18)30273-9. doi:, 10.1016/j.jsb.2018.09.013. PMID:30287387<ref>PMID:30287387</ref>
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Description: SMURFP-Y56R mutant in complex with biliverdin
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From MEDLINE&reg;/PubMed&reg;, a database of the U.S. National Library of Medicine.<br>
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[[Category: Unreleased Structures]]
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</div>
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[[Category: Niessing, D]]
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<div class="pdbe-citations 6fzn" style="background-color:#fffaf0;"></div>
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[[Category: Ntziachristos, V]]
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== References ==
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[[Category: Weidenfeld, I]]
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<references/>
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[[Category: Janowski, R]]
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__TOC__
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[[Category: Richter, K]]
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</StructureSection>
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[[Category: Fuenzalida-Wernera, J.P]]
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[[Category: Large Structures]]
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[[Category: Stiel, A.C]]
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[[Category: Synthetic construct]]
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[[Category: Mishra, K]]
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[[Category: Fuenzalida-Wernera JP]]
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[[Category: Vetschera, P]]
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[[Category: Janowski R]]
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[[Category: Mishra K]]
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[[Category: Niessing D]]
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[[Category: Ntziachristos V]]
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[[Category: Richter K]]
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[[Category: Stiel AC]]
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[[Category: Vetschera P]]
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[[Category: Weidenfeld I]]

Current revision

SMURFP-Y56R mutant in complex with biliverdin

PDB ID 6fzn

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